scholarly journals Microtubule-Actomyosin Mechanical Cooperation during Contact Guidance Sensing

Cell Reports ◽  
2018 ◽  
Vol 25 (2) ◽  
pp. 328-338.e5 ◽  
Author(s):  
Erdem D. Tabdanov ◽  
Vikram Puram ◽  
Alexander Zhovmer ◽  
Paolo P. Provenzano
Keyword(s):  
Contact Guidance

scholarly journals Aligned silk-based 3-D architectures for contact guidance in tissue engineering

2012 ◽  
Vol 8 (4) ◽  
pp. 1530-1542 ◽  
Author(s):  
A.L. Oliveira ◽  
L. Sun ◽  
H.J. Kim ◽  
X. Hu ◽  
W. Rice ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Contact Guidance

scholarly journals Screening Platform for Cell Contact Guidance Based on Inorganic Biomaterial Micro/nanotopographical Gradients

2017 ◽  
Vol 9 (37) ◽  
pp. 31433-31445 ◽  
Author(s):  
Qihui Zhou ◽  
Olga Castañeda Ocampo ◽  
Carlos F. Guimarães ◽  
Philipp T. Kühn ◽  
Theo G. van Kooten ◽  
...  
Keyword(s):  
Contact Guidance ◽  
Cell Contact ◽  
Screening Platform

Surface characterization of poly(methyl methacrylate) microgrooved for contact guidance of mammalian cells

Biomaterials ◽  
2001 ◽  
Vol 22 (12) ◽  
pp. 1635-1642 ◽  
Author(s):  
J.A Alaerts ◽  
V.M De Cupere ◽  
S Moser ◽  
P van den Bosh de Aguilar ◽  
P.G Rouxhet
Keyword(s):  
Methyl Methacrylate ◽  
Mammalian Cells ◽  
Surface Characterization ◽  
Contact Guidance ◽  
Poly Methyl Methacrylate

The sequence of alignment of microtubules, focal contacts and actin filaments in fibroblasts spreading on smooth and grooved titanium substrata

1993 ◽  
Vol 106 (1) ◽  
pp. 343-354 ◽  
Author(s):  
C. Oakley ◽  
D.M. Brunette
Keyword(s):  
Actin Filaments ◽  
Human Gingival Fibroblasts ◽  
Contact Guidance ◽  
Gingival Fibroblasts ◽  
Cell Orientation ◽  
Cell Height ◽  
Focal Contacts ◽  
Microfilament Bundles ◽  
Titanium Surfaces ◽  
Oriented Parallel

Contact guidance refers to the reactions of cells with the topography of their substratum. Current hypotheses on the mechanism of contact guidance focus on the dynamic behaviour of the cytoskeletal components, but most observations have been made on cells that have already become oriented with topographic features of the substratum. The purpose of this study was to examine the sequence in which microtubules, focal contacts and microfilament bundles become aligned to the substratum topography as fibroblasts spread on grooved substrata. Human gingival fibroblasts were trypsinized and seeded onto grooved titanium surfaces produced by micromachining, as well as onto control smooth surfaces. After observation and photography of the spreading cells at times up to 6 hours, the cells were fixed and exposed to one or more of the following antibodies or fluorescent stains: phallacidin to stain actin filaments, monoclonal anti-tubulin, monoclonal anti-vinculin, anti-mouse IgG labelled with Texas-Red or FITC, and/or an aldehyde-reactive stain to identify the cell outline. The cells were photographed and cell area, shape and orientation were calculated. Cells were also examined with confocal microscopy to obtain optical sections so that cell height as well as the precise locations of the cytoskeletal components with respect to the vertical dimension of the grooved substrata could be determined. Microtubules were the first element to become oriented parallel to the direction of the grooves and were first aligned at the bottom of the grooves. This alignment of microtubules was evident as early as 20 minutes after plating and preceded the orientation of the cell as a whole. Aligned actin microfilament bundles were not observed until 40–60 minutes and were observed first at the wall-ridge edges. At early times, focal contacts were distributed radially, but only after 3 hours did the majority of cells demonstrate aligned focal contacts. If the first cytoskeletal component to become aligned is the prime determinant of cell orientation, then these data suggest that microtubules in human gingival fibroblasts may determine cell orientation on grooved titanium surfaces. By analogy with microtubule behaviour in other systems, we suggest that microtubule orientation on grooved substrata may occur as a result of the substratum establishing shear-free planes.

scholarly journals Entropic Forces Drive Cellular Contact Guidance

2019 ◽  
Vol 116 (10) ◽  
pp. 1994-2008 ◽  
Author(s):  
Antonetta B.C. Buskermolen ◽  
Hamsini Suresh ◽  
Siamak S. Shishvan ◽  
Andrea Vigliotti ◽  
Antonio DeSimone ◽  
...  
Keyword(s):  
Contact Guidance ◽  
Cellular Contact

scholarly journals The Effect of the Isomeric Chlorine Substitutions on the Honeycomb-Patterned Films of Poly(x-chlorostyrene)s/Polystyrene Blends and Copolymers via Static Breath Figure Technique

Materials ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 167 ◽  
Author(s):  
Leire Ruiz-Rubio ◽  
Leyre Pérez-Álvarez ◽  
Julia Sanchez-Bodón ◽  
Valeria Arrighi ◽  
José Luis Vilas-Vilela
Keyword(s):  
Voronoi Tessellation ◽  
Contact Guidance ◽  
Polymeric Thin Films ◽  
Styrene Copolymers ◽  
Breath Figure ◽  
Wide Range ◽  
Breath Figure Method ◽  
Patterned Films ◽  
Figure Technique ◽  
Potential Use

Polymeric thin films patterned with honeycomb structures were prepared from poly(x-chlorostyrene) and statistical poly(x-chlorostyrene-co-styrene) copolymers by static breath figure method. Each polymeric sample was synthesized by free radical polymerization and its solution in tetrahydrofuran cast on glass wafers under 90% relative humidity (RH). The effect of the chorine substitution in the topography and conformational entropy was evaluated. The entropy of each sample was calculated by using Voronoi tessellation. The obtained results revealed that these materials could be a suitable toolbox to develop a honeycomb patterns with a wide range of pore sizes for a potential use in contact guidance induced culture.

scholarly journals A Novel Cell Force Sensor for Quantification of Traction during Cell Spreading and Contact Guidance

2007 ◽  
Vol 93 (1) ◽  
pp. 335-345 ◽  
Author(s):  
N. Tymchenko ◽  
J. Wallentin ◽  
S. Petronis ◽  
L.M. Bjursten ◽  
B. Kasemo ◽  
...  
Keyword(s):  
Cell Spreading ◽  
Force Sensor ◽  
Contact Guidance ◽  
Cell Force
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