scholarly journals Screening Platform for Cell Contact Guidance Based on Inorganic Biomaterial Micro/nanotopographical Gradients

2017 ◽  
Vol 9 (37) ◽  
pp. 31433-31445 ◽  
Author(s):  
Qihui Zhou ◽  
Olga Castañeda Ocampo ◽  
Carlos F. Guimarães ◽  
Philipp T. Kühn ◽  
Theo G. van Kooten ◽  
...  
Keyword(s):  
Contact Guidance ◽  
Cell Contact ◽  
Screening Platform

scholarly journals Directional nanotopographic gradients: a high-throughput screening platform for cell contact guidance

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Qihui Zhou ◽  
Philipp T. Kühn ◽  
Thirsa Huisman ◽  
Elsje Nieboer ◽  
Charlotte van Zwol ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Contact Guidance ◽  
Cell Contact ◽  
Screening Platform

Easy fabrication of aligned PLLA nanofibers-based 2D scaffolds suitable for cell contact guidance studies

2016 ◽  
Vol 62 ◽  
pp. 301-306 ◽  
Author(s):  
John Mohanraj ◽  
Luca Puzzi ◽  
Ennio Capria ◽  
Stefania Corvaglia ◽  
Loredana Casalis ◽  
...  
Keyword(s):  
Contact Guidance ◽  
Cell Contact

An Anisotropic Biphasic Theory of Tissue-Equivalent Mechanics: The Interplay Among Cell Traction, Fibrillar Network Deformation, Fibril Alignment, and Cell Contact Guidance

1997 ◽  
Vol 119 (2) ◽  
pp. 137-145 ◽  
Author(s):  
V. H. Barocas ◽  
R. T. Tranquillo
Keyword(s):  
Contact Guidance ◽  
Deformation Tensor ◽  
Cell Contact ◽  
Cell Alignment ◽  
Collagen Gels ◽  
Cell Orientation ◽  
Collagen Network ◽  
Orientation Tensor ◽  
Tissue Equivalents ◽  
Cell Traction

We present a general mathematical theory for the mechanical interplay in tissue-equivalents (cell-populated collagen gels): Cell traction leads to compaction of the fibrillar collagen network, which for certain conditions such as a mechanical constraint or inhomogeneous cell distribution, can result in inhomogeneous compaction and consequently fibril alignment, leading to cell contact guidance, which affects the subsequent compaction. The theory accounts for the intrinsically biphasic nature of collagen gel, which is comprised of collagen network and interstitial solution. The theory also accounts for fibril alignment due to inhomogeneous network deformation, that is, anisotropic strain, and for cell alignment in response to fibril alignment. Cell alignment results in anisotropic migration and traction, as modeled by a cell orientation tensor that is a function of a fiber orientation tensor, which is defined by the network deformation tensor. Models for a variety of tissue-equivalents are shown to predict qualitatively the alignment that arises due to inhomogeneous compaction driven by cell traction.

Directing Osteoblast Alignment and Elongation on the Micro-Grooved Silica-Based Hybrid Membrane

2013 ◽  
Vol 647 ◽  
pp. 165-169 ◽  
Author(s):  
Song Chen ◽  
Nobutaka Hanagata
Keyword(s):  
Bone Tissue ◽  
Potential Application ◽  
Hybrid Membrane ◽  
Contact Guidance ◽  
Cell Contact ◽  
Hybrid Membranes ◽  
Pdms Mold

Silica-chitosan hybrid was synthesized from a mixture of chitosan and glycidyloxypropyltrimethoxysilane and then coated on a microgrooved polydimethylsiloxane (PDMS) mold to produce the microgrooved silica-chitosan hybrid membrane. After incubated with osteoblast-like MC3T3-E1 cells, the microgrooved hybrid membranes strongly directed the alignment and elongation of cells and exhibited a strong cell contact guidance ability, indicating a potential application as engineering bone tissue regenerative materials.

Response of single, pairs, and clusters of epithelial cells to substratum topography

1995 ◽  
Vol 73 (7-8) ◽  
pp. 473-489 ◽  
Author(s):  
C. Oakley ◽  
D. M. Brunette
Keyword(s):  
Epithelial Cells ◽  
Actin Filaments ◽  
Cell Shape ◽  
Single Cells ◽  
Cell Spreading ◽  
Contact Guidance ◽  
Adjacent Cell ◽  
Cell Contact ◽  
Topographic Effects ◽  
Control Surfaces

Cells cultured on grooved substrata change their shape, orientation, and direction of locomotion in response to substratum topography, a phenomenon called contact or topographic guidance. Porcine epithelial cells (E-cells) spread on micromachined grooved or smooth control surfaces were examined by epifluorescence and confocal microscopy to determine area, cell shape, and orientation in conjunction with distributions and orientations of actin filaments and microtubules. Single cells, cells within a pair or cluster, and pairs or clusters considered as a unit were compared. As expected, cell contact increased cell spreading, but surprisingly, increased cell contact influenced cell shape on smooth and grooved surfaces and increased alignment of cells spread on grooves. Both actin filaments and microtubules aligned initially and most consistently along the walls and ridge–groove edges. Single E-cells displayed the least variability of aligned cytoskeletal patterns. E-cells within clusters displayed the most variability as local topographic effects on the cytoskeleton could be overridden by adjacent cell contact. Overall, contact guidance of E-cells was neither synonymous with nor contingent upon an elliptical morphology oriented to the topography. E-cells also differed from fibroblasts in their response to cell contact and in their lack of a relationship between cell polarity and locomotion.Key words: microtubules, actin, topographic guidance, micromachined substrata.

Schwann Cell Contact Guidance versus Boundary ­Interaction in Functional Wound Healing along Nano and Microstructured Membranes

2015 ◽  
Vol 4 (12) ◽  
pp. 1849-1860 ◽  
Author(s):  
Ilaria Tonazzini ◽  
Emanuela Jacchetti ◽  
Sandro Meucci ◽  
Fabio Beltram ◽  
Marco Cecchini
Keyword(s):  
Wound Healing ◽  
Schwann Cell ◽  
Contact Guidance ◽  
Cell Contact ◽  
Boundary Interaction

scholarly journals Cell contact guidance via sensing anisotropy of network mechanical resistance

2021 ◽  
Vol 118 (29) ◽  
pp. e2024942118
Author(s):  
Greeshma Thrivikraman ◽  
Alicja Jagiełło ◽  
Victor K. Lai ◽  
Sandra L. Johnson ◽  
Mark Keating ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Mammalian Cells ◽  
Dermal Fibroblasts ◽  
Contact Guidance ◽  
Cell Contact ◽  
Mechanical Resistance ◽  
Cell Orientation ◽  
Fibrin Gel ◽  
Anisotropy Index ◽  
The Cross

Despite the ubiquitous importance of cell contact guidance, the signal-inducing contact guidance of mammalian cells in an aligned fibril network has defied elucidation. This is due to multiple interdependent signals that an aligned fibril network presents to cells, including, at least, anisotropy of adhesion, porosity, and mechanical resistance. By forming aligned fibrin gels with the same alignment strength, but cross-linked to different extents, the anisotropic mechanical resistance hypothesis of contact guidance was tested for human dermal fibroblasts. The cross-linking was shown to increase the mechanical resistance anisotropy, without detectable change in network microstructure and without change in cell adhesion to the cross-linked fibrin gel. This methodology thus isolated anisotropic mechanical resistance as a variable for fixed anisotropy of adhesion and porosity. The mechanical resistance anisotropy |Y*|−1 − |X*|−1 increased over fourfold in terms of the Fourier magnitudes of microbead displacement |X*| and |Y*| at the drive frequency with respect to alignment direction Y obtained by optical forces in active microrheology. Cells were found to exhibit stronger contact guidance in the cross-linked gels possessing greater mechanical resistance anisotropy: the cell anisotropy index based on the tensor of cell orientation, which has a range 0 to 1, increased by 18% with the fourfold increase in mechanical resistance anisotropy. We also show that modulation of adhesion via function-blocking antibodies can modulate the guidance response, suggesting a concomitant role of cell adhesion. These results indicate that fibroblasts can exhibit contact guidance in aligned fibril networks by sensing anisotropy of network mechanical resistance.

scholarly journals The Role of Non-Muscle Myosin 2A and 2B in the Regulation of Mesenchymal Cell Contact Guidance

2019 ◽  
Vol 116 (3) ◽  
pp. 259a
Author(s):  
Alexander S. Zhovmer ◽  
Erdem Tabdanov ◽  
Houxun Miao ◽  
Han Wen ◽  
Jinqiu Chen ◽  
...  
Keyword(s):  
Mesenchymal Cell ◽  
Contact Guidance ◽  
Cell Contact ◽  
Muscle Myosin
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