scholarly journals Aligned silk-based 3-D architectures for contact guidance in tissue engineering

2012 ◽  
Vol 8 (4) ◽  
pp. 1530-1542 ◽  
Author(s):  
A.L. Oliveira ◽  
L. Sun ◽  
H.J. Kim ◽  
X. Hu ◽  
W. Rice ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Contact Guidance

Influence of Strain and Contact Guidance on Collagen Organization in Engineered Cardiovascular Tissues: Implications for In Situ Tissue Engineering

2013 ◽  
Author(s):  
Nicky de Jonge ◽  
Giulia Argento ◽  
Frank P. T. Baaijens ◽  
Carlijn V. C. Bouten
Keyword(s):  
Tissue Engineering ◽  
Collagen Content ◽  
Contact Guidance ◽  
Load Bearing ◽  
Collagen Remodeling ◽  
Cardiovascular Tissues ◽  
Scaffold Structure ◽  
Degradation Properties ◽  
In Situ Tissue Engineering

Cardiovascular tissues have a prominent load-bearing function. Collagen fibers in the extracellular matrix provide strength to these tissues. In particular the content and organization of these fibers contribute to overall strength [1]. In case of changes in mechanical demand, collagen content and organization can be adapted; a process referred to as collagen remodeling. For the creation of engineered cardiovascular tissues knowledge about collagen remodeling is of utmost importance to produce tissues with load bearing function. In case of in situ tissue engineering (TE) collagen content and organization in the developing tissue can be influenced by local tissue strains as well as scaffold structure and degradation properties [2, 3].

scholarly journals Contact guidance for cardiac tissue engineering using 3D bioprinted gelatin patterned hydrogel

2018 ◽  
Vol 10 (2) ◽  
pp. 025003 ◽  
Author(s):  
Ajay Tijore ◽  
Scott Alexander Irvine ◽  
Udi Sarig ◽  
Priyadarshini Mhaisalkar ◽  
Vrushali Baisane ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Contact Guidance

Neural tissue engineering: the influence of scaffold surface topography and extracellular matrix microenvironment

2021 ◽  
Author(s):  
Chun-Yi Yang ◽  
Wei-Yuan Huang ◽  
Liang-Hsin Chen ◽  
Nai-Wen Liang ◽  
Huan-Chih Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Surface Topography ◽  
Neural Tissue ◽  
Contact Guidance ◽  
Neural Tissue Engineering

Strategies using surface topography, contact guidance and biomechanical cues in the design of scaffolds as an ECM support for neural tissue engineering.

Contact Guidance by Microstructured Gelatin Hydrogels for Prospective Tissue Engineering Applications

2019 ◽  
Vol 11 (7) ◽  
pp. 7450-7458 ◽  
Author(s):  
Meike Tadsen ◽  
Ralf P. Friedrich ◽  
Stefanie Riedel ◽  
Christoph Alexiou ◽  
Stefan G. Mayr
Keyword(s):  
Tissue Engineering ◽  
Contact Guidance ◽  
Engineering Applications

scholarly journals The Research Advance of Cell Bridges in vitro

2020 ◽  
Vol 8 ◽  
Author(s):  
Qing Zhang
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Mechanical Stability ◽  
Three Dimensional ◽  
Contact Guidance ◽  
The Novel ◽  
Physiological Processes ◽  
Medical Materials

The microenvironment in which cells reside in vivo dictates their biological and mechanical functioning is associated with morphogenetic and regenerative processes and may find implications in regenerative medicine and tissue engineering. The development of nano- and micro-fabricated technologies, three-dimensional (3D) printing technique, and biomimetic medical materials have enabled researchers to prepare novel advanced substrates mimicking the in vivo microenvironment. Most of the novel morphologies and behaviors of cells, including contact guidance and cell bridges which are observed in vivo but are not perceived in the traditional two-dimensional (2D) culture system, emerged on those novel substrates. Using cell bridges, cell can span over the surface of substrates to maintain mechanical stability and integrity of tissue, as observed in physiological processes, such as wound healing, regeneration and development. Compared to contact guidance, which has received increased attention and is investigated extensively, studies on cell bridges remain scarce. Therefore, in this mini-review, we have comprehensively summarized and classified different kinds of cell bridges formed on various substrates and highlighted possible biophysical mechanisms underlying cell bridge formation for their possible implication in the fields of tissue engineering and regenerative medicine.

scholarly journals PHBV/PAM Scaffolds with Local Oriented Structure through UV Polymerization for Tissue Engineering

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Yu Ke ◽  
Gang Wu ◽  
Yingjun Wang
Keyword(s):  
Tissue Engineering ◽  
Elastic Modulus ◽  
Graft Polymerization ◽  
Contact Guidance ◽  
Tissue Engineering Scaffolds ◽  
Lower Porosity ◽  
Exciting Potential ◽  
Direct Cell ◽  
And Migration ◽  
Cell Spread

Locally oriented tissue engineering scaffolds can provoke cellular orientation and direct cell spread and migration, offering an exciting potential way for the regeneration of the complex tissue. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) scaffolds with locally oriented hydrophilic polyacrylamide (PAM) inside the macropores of the scaffolds were achieved through UV graft polymerization. The interpenetrating PAM chains enabled good interconnectivity of PHBV/PAM scaffolds that presented a lower porosity and minor diameter of pores than PHBV scaffolds. The pores with diameter below 100 μm increased to 82.15% of PHBV/PAM scaffolds compared with 31.5% of PHBV scaffolds. PHBV/PAM scaffold showed a much higher compressive elastic modulus than PHBV scaffold due to PAM stuffing. At 5 days of culturing, sheep chondrocytes spread along the similar direction in the macropores of PHBV/PAM scaffolds. The locally oriented PAM chains might guide the attachment and spreading of chondrocytes and direct the formation of microfilamentsviacontact guidance.

Decellularized bone extracellular matrix in skeletal tissue engineering

2020 ◽  
Vol 48 (3) ◽  
pp. 755-764
Author(s):  
Benjamin B. Rothrauff ◽  
Rocky S. Tuan
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Tissue Regeneration ◽  
Animal Studies ◽  
Tumor Resection ◽  
Regenerative Capacity ◽  
Skeletal Tissue ◽  
Large Bone

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

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