scholarly journals Long-Term Calorie Restriction Enhances Cellular Quality-Control Processes in Human Skeletal Muscle

Cell Reports ◽  
2016 ◽  
Vol 14 (3) ◽  
pp. 422-428 ◽  
Author(s):  
Ling Yang ◽  
Danilo Licastro ◽  
Edda Cava ◽  
Nicola Veronese ◽  
Francesco Spelta ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Quality Control ◽  
Calorie Restriction ◽  
Human Skeletal Muscle ◽  
Control Processes

scholarly journals Effect of ionizing radiation on human skeletal muscle precursor cells

2013 ◽  
Vol 47 (4) ◽  
pp. 376-381 ◽  
Author(s):  
Mihaela Jurdana ◽  
Maja Cemazar ◽  
Katarina Pegan ◽  
Tomaz Mars
Keyword(s):  
Skeletal Muscle ◽  
Stress Response ◽  
Ionizing Radiation ◽  
Human Skeletal Muscle ◽  
Long Term Effects ◽  
Post Irradiation ◽  
Acute Response ◽  
Proliferation Capacity ◽  
Myoblast Proliferation

Abstract Background. Long term effects of different doses of ionizing radiation on human skeletal muscle myoblast proliferation, cytokine signalling and stress response capacity were studied in primary cell cultures. Materials and methods. Human skeletal muscle myoblasts obtained from muscle biopsies were cultured and irradiated with a Darpac 2000 X-ray unit at doses of 4, 6 and 8 Gy. Acute effects of radiation were studied by interleukin - 6 (IL-6) release and stress response detected by the heat shock protein (HSP) level, while long term effects were followed by proliferation capacity and cell death. Results. Compared with non-irradiated control and cells treated with inhibitor of cell proliferation Ara C, myoblast proliferation decreased 72 h post-irradiation, this effect was more pronounced with increasing doses. Post-irradiation myoblast survival determined by measurement of released LDH enzyme activity revealed increased activity after exposure to irradiation. The acute response of myoblasts to lower doses of irradiation (4 and 6 Gy) was decreased secretion of constitutive IL-6. Higher doses of irradiation triggered a stress response in myoblasts, determined by increased levels of stress markers (HSPs 27 and 70). Conclusions. Our results show that myoblasts are sensitive to irradiation in terms of their proliferation capacity and capacity to secret IL-6. Since myoblast proliferation and differentiation are a key stage in muscle regeneration, this effect of irradiation needs to be taken in account, particularly in certain clinical conditions.

Erythropoietin receptor in human skeletal muscle and the effects of acute and long-term injections with recombinant human erythropoietin on the skeletal muscle

2008 ◽  
Vol 104 (4) ◽  
pp. 1154-1160 ◽  
Author(s):  
Carsten Lundby ◽  
Ylva Hellsten ◽  
Mie B. F. Jensen ◽  
Anders S. Munch ◽  
Henriette Pilegaard
Keyword(s):  
Skeletal Muscle ◽  
Transferrin Receptor ◽  
Human Skeletal Muscle ◽  
Physiological Role ◽  
Cell Types ◽  
Erythropoietin Receptor ◽  
Mrna Levels ◽  
Human Erythropoietin ◽  
Plasma Hormones

The presence and potential physiological role of the erythropoietin receptor (Epo-R) were examined in human skeletal muscle. In this study we demonstrate that Epo-R is present in the endothelium, smooth muscle cells, and in fractions of the sarcolemma of skeletal muscle fibers. To study the potential effects of Epo in human skeletal muscle, two separate studies were conducted: one to study the acute effects of a single Epo injection on skeletal muscle gene expression and plasma hormones and another to study the effects of long-term (14 wk) Epo treatment on skeletal muscle structure. Subjects ( n = 11) received a single Epo injection of 15,000 IU (double blinded, cross over, placebo). A single Epo injection reduced myoglobin and increased transferrin receptor and MRF-4 mRNA content within 10 h after injection. Plasma hormones remained unaltered. Capillarization and fiber hypertrophy was studied in subjects ( n = 8) who received long-term Epo administration, and muscle biopsies were obtained before and after. Epo treatment did not alter mean fiber area (0.84 ± 0.2 vs. 0.72 ± 0.3 mm2), capillaries per fiber (4.3 ± 0.5 vs. 4.4 ± 1.3), or number of proliferating endothelial cells. In conclusion, the Epo-R is present in the vasculature and myocytes in human skeletal muscle, suggesting a role in both cell types. In accordance, a single injection of Epo regulates myoglobin, MRF-4, and transferrin receptor mRNA levels. However, in contrast to our hypothesis, prolonged Epo administration had no apparent effect on capillarization or muscle fiber hypertrophy.

scholarly journals Effects of Long Term Supplementation of Anabolic Androgen Steroids on Human Skeletal Muscle

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e105330 ◽  
Author(s):  
Ji-Guo Yu ◽  
Patrik Bonnerud ◽  
Anders Eriksson ◽  
Per S. Stål ◽  
Yelverton Tegner ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Anabolic Androgen

scholarly journals Expression of striated activator of rho-signaling in human skeletal muscle following acute exercise and long-term training

2018 ◽  
Vol 6 (5) ◽  
pp. e13624 ◽  
Author(s):  
Stefan M. Reitzner ◽  
Jessica Norrbom ◽  
Carl Johan Sundberg ◽  
Eva-Karin Gidlund
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Acute Exercise

scholarly journals The effect of acute and long-term physical activity on extracellular matrix and serglycin in human skeletal muscle

2015 ◽  
Vol 3 (8) ◽  
pp. e12473 ◽  
Author(s):  
Marit Hjorth ◽  
Frode Norheim ◽  
Astri J. Meen ◽  
Shirin Pourteymour ◽  
Sindre Lee ◽  
...  
Keyword(s):  
Physical Activity ◽  
Skeletal Muscle ◽  
Extracellular Matrix ◽  
Human Skeletal Muscle

scholarly journals Human skeletal muscle HSP70 response to training in highly trained rowers

1999 ◽  
Vol 86 (1) ◽  
pp. 101-104 ◽  
Author(s):  
Yuefei Liu ◽  
Sabine Mayr ◽  
Alexandra Opitz-Gress ◽  
Claudia Zeller ◽  
Werner Lormes ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Heat Shock ◽  
Heat Shock Protein 70 ◽  
Human Skeletal Muscle ◽  
Vastus Lateralis ◽  
Human Muscle ◽  
Muscle Sample ◽  
Exercise Induced ◽  
The Right

Previous studies have demonstrated exercise-induced heat shock protein 70 (HSP70) in animals. The purpose of this study was to investigate human skeletal muscle HSP70 response to rowing training. Ten male rowers trained for 4 wk with different forms, durations, and intensities of exercise. Biopsy was performed in the right musculus vastus lateralis before training and at the end of each week. HSP70 in 5 μg of total protein from the muscle sample was determined by using Western blot and immunodetection with chemiluminescence technique, by means of laser densitometer referring to a series of known standard HSP70. Compared with pretraining (100%), HSP70 increased during training (181, 405, 456, and 363% from the first to fourth training week, respectively) with the maximum HSP70 production at the end of second training week. Thus HSP70 is induced in highly trained human muscle by long-term training.

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