Changes in human skeletal muscle induced by long-term eccentric exercise

1984 ◽  
Vol 236 (2) ◽  
Author(s):  
Jan Frid�n
Keyword(s):  
Skeletal Muscle ◽  
Eccentric Exercise ◽  
Human Skeletal Muscle

scholarly journals Effect of ionizing radiation on human skeletal muscle precursor cells

2013 ◽  
Vol 47 (4) ◽  
pp. 376-381 ◽  
Author(s):  
Mihaela Jurdana ◽  
Maja Cemazar ◽  
Katarina Pegan ◽  
Tomaz Mars
Keyword(s):  
Skeletal Muscle ◽  
Stress Response ◽  
Ionizing Radiation ◽  
Human Skeletal Muscle ◽  
Long Term Effects ◽  
Post Irradiation ◽  
Acute Response ◽  
Proliferation Capacity ◽  
Myoblast Proliferation

Abstract Background. Long term effects of different doses of ionizing radiation on human skeletal muscle myoblast proliferation, cytokine signalling and stress response capacity were studied in primary cell cultures. Materials and methods. Human skeletal muscle myoblasts obtained from muscle biopsies were cultured and irradiated with a Darpac 2000 X-ray unit at doses of 4, 6 and 8 Gy. Acute effects of radiation were studied by interleukin - 6 (IL-6) release and stress response detected by the heat shock protein (HSP) level, while long term effects were followed by proliferation capacity and cell death. Results. Compared with non-irradiated control and cells treated with inhibitor of cell proliferation Ara C, myoblast proliferation decreased 72 h post-irradiation, this effect was more pronounced with increasing doses. Post-irradiation myoblast survival determined by measurement of released LDH enzyme activity revealed increased activity after exposure to irradiation. The acute response of myoblasts to lower doses of irradiation (4 and 6 Gy) was decreased secretion of constitutive IL-6. Higher doses of irradiation triggered a stress response in myoblasts, determined by increased levels of stress markers (HSPs 27 and 70). Conclusions. Our results show that myoblasts are sensitive to irradiation in terms of their proliferation capacity and capacity to secret IL-6. Since myoblast proliferation and differentiation are a key stage in muscle regeneration, this effect of irradiation needs to be taken in account, particularly in certain clinical conditions.

scholarly journals Long-Term Calorie Restriction Enhances Cellular Quality-Control Processes in Human Skeletal Muscle

Cell Reports ◽  
2016 ◽  
Vol 14 (3) ◽  
pp. 422-428 ◽  
Author(s):  
Ling Yang ◽  
Danilo Licastro ◽  
Edda Cava ◽  
Nicola Veronese ◽  
Francesco Spelta ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Quality Control ◽  
Calorie Restriction ◽  
Human Skeletal Muscle ◽  
Control Processes

Eccentric exercise markedly increases c-Jun NH2-terminal kinase activity in human skeletal muscle

1999 ◽  
Vol 87 (5) ◽  
pp. 1668-1673 ◽  
Author(s):  
Marni D. Boppart ◽  
Doron Aronson ◽  
Lindsay Gibson ◽  
Ronenn Roubenoff ◽  
Leslie W. Abad ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Protein Kinase ◽  
Eccentric Exercise ◽  
Intracellular Signaling ◽  
Human Skeletal Muscle ◽  
Vastus Lateralis ◽  
Fold Increase ◽  
Mitogen Activated Protein Kinase ◽  
Eccentric Contractions ◽  
Vastus Lateralis Muscle

Eccentric contractions require the lengthening of skeletal muscle during force production and result in acute and prolonged muscle injury. Because a variety of stressors, including physical exercise and injury, can result in the activation of the c-Jun NH2-terminal kinase (JNK) intracellular signaling cascade in skeletal muscle, we investigated the effects of eccentric exercise on the activation of this stress-activated protein kinase in human skeletal muscle. Twelve healthy subjects (7 men, 5 women) completed maximal concentric or eccentric knee extensions on a KinCom isokinetic dynamometer (10 sets, 10 repetitions). Percutaneous needle biopsies were obtained from the vastus lateralis muscle 24 h before exercise (basal), immediately postexercise, and 6 h postexercise. Whereas both forms of exercise increased JNK activity immediately postexercise, eccentric contractions resulted in a much higher activation (15.4 ± 4.5 vs. 3.5 ± 1.4-fold increase above basal, eccentric vs. concentric). By 6 h after exercise, JNK activity decreased back to baseline values. In contrast to the greater activation of JNK with eccentric exercise, the mitogen-activated protein kinase kinase 4, the immediate upstream regulator of JNK, was similarly activated by concentric and eccentric exercise. Because the activation of JNK promotes the phosphorylation of a variety of transcription factors, including c-Jun, the results from this study suggest that JNK may be involved in the molecular and cellular adaptations that occur in response to injury-producing exercise in human skeletal muscle.

The heat shock protein response following eccentric exercise in human skeletal muscle is unaffected by local NSAID infusion

2013 ◽  
Vol 113 (7) ◽  
pp. 1883-1893 ◽  
Author(s):  
U. R. Mikkelsen ◽  
G. Paulsen ◽  
P. Schjerling ◽  
I. C. Helmark ◽  
H. Langberg ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Eccentric Exercise ◽  
Human Skeletal Muscle ◽  
Protein Response

ECCENTRIC EXERCISE POTENTLY ACTIVATES c-JUN NH2 TERMINAL KINASE(JNK) SIGNALING IN HUMAN SKELETAL MUSCLE

1998 ◽  
Vol 30 (Supplement) ◽  
pp. 2
Author(s):  
M. D. Boppart ◽  
L. Gibson ◽  
D. Aronson ◽  
J. Bean ◽  
L. J. Goodyear ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Eccentric Exercise ◽  
Human Skeletal Muscle ◽  
Jnk Signaling

scholarly journals Immunological changes in human skeletal muscle and blood after eccentric exercise and multiple biopsies

2000 ◽  
Vol 529 (1) ◽  
pp. 243-262 ◽  
Author(s):  
Christer Malm ◽  
Pernilla Nyberg ◽  
Marianne Engström ◽  
Bertil Sjödin ◽  
Rodica Lenkei ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Eccentric Exercise ◽  
Human Skeletal Muscle ◽  
Multiple Biopsies ◽  
Immunological Changes

Activation of NF-kB in Pericytes of Human Skeletal Muscle Following Eccentric Exercise-Induced Damage

2010 ◽  
Vol 42 ◽  
pp. 96 ◽  
Author(s):  
Robert D. Hyldahl ◽  
Ling Xin ◽  
Monica J. Hubal ◽  
Stephanie Moekel-Cole ◽  
Stuart Chipkin ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Eccentric Exercise ◽  
Human Skeletal Muscle ◽  
Exercise Induced
Sign in / Sign up

Export Citation Format

Share Document