scholarly journals The effect of acute and long-term physical activity on extracellular matrix and serglycin in human skeletal muscle

2015 ◽  
Vol 3 (8) ◽  
pp. e12473 ◽  
Author(s):  
Marit Hjorth ◽  
Frode Norheim ◽  
Astri J. Meen ◽  
Shirin Pourteymour ◽  
Sindre Lee ◽  
...  
Keyword(s):  
Physical Activity ◽  
Skeletal Muscle ◽  
Extracellular Matrix ◽  
Human Skeletal Muscle

scholarly journals Effect of ionizing radiation on human skeletal muscle precursor cells

2013 ◽  
Vol 47 (4) ◽  
pp. 376-381 ◽  
Author(s):  
Mihaela Jurdana ◽  
Maja Cemazar ◽  
Katarina Pegan ◽  
Tomaz Mars
Keyword(s):  
Skeletal Muscle ◽  
Stress Response ◽  
Ionizing Radiation ◽  
Human Skeletal Muscle ◽  
Long Term Effects ◽  
Post Irradiation ◽  
Acute Response ◽  
Proliferation Capacity ◽  
Myoblast Proliferation

Abstract Background. Long term effects of different doses of ionizing radiation on human skeletal muscle myoblast proliferation, cytokine signalling and stress response capacity were studied in primary cell cultures. Materials and methods. Human skeletal muscle myoblasts obtained from muscle biopsies were cultured and irradiated with a Darpac 2000 X-ray unit at doses of 4, 6 and 8 Gy. Acute effects of radiation were studied by interleukin - 6 (IL-6) release and stress response detected by the heat shock protein (HSP) level, while long term effects were followed by proliferation capacity and cell death. Results. Compared with non-irradiated control and cells treated with inhibitor of cell proliferation Ara C, myoblast proliferation decreased 72 h post-irradiation, this effect was more pronounced with increasing doses. Post-irradiation myoblast survival determined by measurement of released LDH enzyme activity revealed increased activity after exposure to irradiation. The acute response of myoblasts to lower doses of irradiation (4 and 6 Gy) was decreased secretion of constitutive IL-6. Higher doses of irradiation triggered a stress response in myoblasts, determined by increased levels of stress markers (HSPs 27 and 70). Conclusions. Our results show that myoblasts are sensitive to irradiation in terms of their proliferation capacity and capacity to secret IL-6. Since myoblast proliferation and differentiation are a key stage in muscle regeneration, this effect of irradiation needs to be taken in account, particularly in certain clinical conditions.

scholarly journals Long-Term Calorie Restriction Enhances Cellular Quality-Control Processes in Human Skeletal Muscle

Cell Reports ◽  
2016 ◽  
Vol 14 (3) ◽  
pp. 422-428 ◽  
Author(s):  
Ling Yang ◽  
Danilo Licastro ◽  
Edda Cava ◽  
Nicola Veronese ◽  
Francesco Spelta ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Quality Control ◽  
Calorie Restriction ◽  
Human Skeletal Muscle ◽  
Control Processes

scholarly journals Sequenced response of extracellular matrix deadhesion and fibrotic regulators after muscle damage is involved in protection against future injury in human skeletal muscle

2011 ◽  
Vol 25 (6) ◽  
pp. 1943-1959 ◽  
Author(s):  
Abigail L. Mackey ◽  
Simon Brandstetter ◽  
Peter Schjerling ◽  
Jens Bojsen‐Moller ◽  
Klaus Qvortrup ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Extracellular Matrix ◽  
Muscle Damage ◽  
Human Skeletal Muscle

scholarly journals Perilipin 4 in human skeletal muscle: localization and effect of physical activity

2015 ◽  
Vol 3 (8) ◽  
pp. e12481 ◽  
Author(s):  
Shirin Pourteymour ◽  
Sindre Lee ◽  
Torgrim M. Langleite ◽  
Kristin Eckardt ◽  
Marit Hjorth ◽  
...  
Keyword(s):  
Physical Activity ◽  
Skeletal Muscle ◽  
Human Skeletal Muscle

Roles of sedentary aging and lifelong physical activity in exchange of glutathione across exercising human skeletal muscle

2014 ◽  
Vol 73 ◽  
pp. 166-173 ◽  
Author(s):  
Michael Nyberg ◽  
Stefan P. Mortensen ◽  
Helena Cabo ◽  
Mari-Carmen Gomez-Cabrera ◽  
Jose Viña ◽  
...  
Keyword(s):  
Physical Activity ◽  
Skeletal Muscle ◽  
Human Skeletal Muscle

scholarly journals Depletion of resident muscle stem cells negatively impacts running volume, physical function, and muscle fiber hypertrophy in response to lifelong physical activity

2020 ◽  
Vol 318 (6) ◽  
pp. C1178-C1188 ◽  
Author(s):  
Davis A. Englund ◽  
Kevin A. Murach ◽  
Cory M. Dungan ◽  
Vandré C. Figueiredo ◽  
Ivan J. Vechetti ◽  
...  
Keyword(s):  
Physical Activity ◽  
Skeletal Muscle ◽  
Physical Function ◽  
Satellite Cell ◽  
Muscle Fiber ◽  
Satellite Cells ◽  
Cell Depletion ◽  
Muscle Adaptation ◽  
Adult Skeletal Muscle

To date, studies that have aimed to investigate the role of satellite cells during adult skeletal muscle adaptation and hypertrophy have utilized a nontranslational stimulus and/or have been performed over a relatively short time frame. Although it has been shown that satellite cell depletion throughout adulthood does not drive skeletal muscle loss in sedentary mice, it remains unknown how satellite cells participate in skeletal muscle adaptation to long-term physical activity. The current study was designed to determine whether reduced satellite cell content throughout adulthood would influence the transcriptome-wide response to physical activity and diminish the adaptive response of skeletal muscle. We administered vehicle or tamoxifen to adult Pax7-diphtheria toxin A (DTA) mice to deplete satellite cells and assigned them to sedentary or wheel-running conditions for 13 mo. Satellite cell depletion throughout adulthood reduced balance and coordination, overall running volume, and the size of muscle proprioceptors (spindle fibers). Furthermore, satellite cell participation was necessary for optimal muscle fiber hypertrophy but not adaptations in fiber type distribution in response to lifelong physical activity. Transcriptome-wide analysis of the plantaris and soleus revealed that satellite cell function is muscle type specific; satellite cell-dependent myonuclear accretion was apparent in oxidative muscles, whereas initiation of G protein-coupled receptor (GPCR) signaling in the glycolytic plantaris may require satellite cells to induce optimal adaptations to long-term physical activity. These findings suggest that satellite cells play a role in preserving physical function during aging and influence muscle adaptation during sustained periods of physical activity.

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