Morphological and functional changes of dissociated single pancreatic acinar cells: testing the suitability of the single cell as a model for exocytosis and calcium signaling

Cell Calcium ◽  
2004 ◽  
Vol 35 (4) ◽  
pp. 367-379 ◽  
Author(s):  
Myoung Kyu Park ◽  
Misun Lee ◽  
Ole H Petersen
Keyword(s):  
Calcium Signaling ◽  
Single Cell ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells ◽  
Functional Changes

Oscillatory mode of calcium signaling in rat pancreatic acinar cells

1990 ◽  
Vol 258 (1) ◽  
pp. C147-C155 ◽  
Author(s):  
Y. Tsunoda ◽  
E. L. Stuenkel ◽  
J. A. Williams
Keyword(s):  
Calcium Signaling ◽  
Calcium Concentration ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells ◽  
Free Calcium ◽  
Subsequent Increase ◽  
High K ◽  
Free Calcium Concentration ◽  
High Concentrations ◽  
Sustained Increase

Cytoplasmic free calcium concentration ((Ca2+]i) was evaluated by dual-wavelength microspectrofluorometry of fura-2-loaded individual rat pancreatic acinar cells. Resting [Ca2+]i in unstimulated acini was 94.1 +/- 4.1 nM. Stimulation with high concentrations of cholecystokinin (CCK, 100 pM to 1 nM) led to an immediate rise in [Ca2+]i to 400-1,000 nM followed by a fall within 2-5 min to a plateau only slightly above the prestimulation level. Lower and more physiological concentrations of CCK (1-30 pM), after a latent period of 60-90 s, induced a smaller sustained increase in [Ca2+]i (30-40 nM) with superimposed repetitive transient [Ca2+]i spikes. These oscillations averaged 120-150 nM in amplitude, occurred at a frequency which averaged 1.5 times/min, and were maintained as long as the stimulus was applied. Similar [Ca2+]i oscillations were observed when acini were stimulated with submaximal concentrations of carbamylcholine (0.1-1 microM) and neuromedin C (0.1-1 nM). Intracellular Ca2+ stores were not depleted during [Ca2+] oscillations, since a subsequent increase to 1 nM CCK led to an immediate rise in [Ca2+]i indistinguishable from the response of cells initially stimulated at this concentration. Although extracellular Ca2+ was required for maintenance of frequency of the spikes, the major source of Ca2+ utilized for oscillations was intracellular, since elimination of medium Ca2+ or Ca2+ entry blockade with lanthanum failed to inhibit oscillations. Vasoactive intestinal polypeptide (10 nM) and high K+ (50 mM) did not affect [Ca2+]i oscillations. Antimycin (10 microM), which depletes cytoplasmic ATP, increased basal [Ca2+]i and inhibited the oscillations.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Mechanisms of damage of acinar pancreatic cells in acute alcohol pancreatitis

2019 ◽  
Vol 16 (1) ◽  
pp. 108-116
Author(s):  
L. A. Mozheiko
Keyword(s):  
Molecular Level ◽  
Early Stage ◽  
Acinar Cells ◽  
Current Data ◽  
Cellular Damage ◽  
Pancreatic Acinar Cells ◽  
Atp Production ◽  
Functional Changes ◽  
Trypsinogen Activation ◽  
Pancreatic Cells

The review analyzes the current data on the main mechanisms of toxic effects of alcohol and its metabolites on pancreatic acinar cells in acute pancreatitis. It is shown that the mechanisms of cellular damage are multicomponent and closely linked by the regulatory factors of the molecular level. At the early stage of the disease, they lead to the following structural and functional changes in acinar cells that promote the premature intracellular trypsinogen activation and autoaggression: sustained rise of cytosolic Ca2+ and excess of mitochondrial matrix Ca2+; destabilization due to lysosomes and zymogen granules; debective autophagy; mitochondrial depolarization; decreased ATP production and necrosis.

scholarly journals RNA-seq library preparation from single pancreatic acinar cells

2016 ◽  
Author(s):  
Damian Wollny ◽  
Sheng Zhao ◽  
Ana Martin-Villalba
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Acinar Cells ◽  
Cell Types ◽  
Cellular Heterogeneity ◽  
Pancreatic Acinar Cells ◽  
Library Preparation ◽  
Cell Type ◽  
Promising Tool ◽  
Single Cell Rna Sequencing

Single cell RNA sequencing technology has emerged as a promising tool to uncover previously neglected cellular heterogeneity. Multiple methods and protocols have been developed to apply single cell sequencing to different cell types from various organs. However, library preparation for RNA sequencing remains challenging for cell types with high RNAse content due to rapid degradation of endogenous RNA molecules upon cell lysis. To this end, we developed a protocol based on the SMART-seq2 technology for single cell RNA sequencing of pancreatic acinar cells, the cell type with one of the highest ribonuclease concentration measured to date. This protocol reliably produces high quality libraries from single acinar cells reaching a total of 5x106 reads / cell and ∼ 80% transcript mapping rate with no detectable 3´end bias. Thus, our protocol makes single cell transcriptomics accessible to cell type with very high RNAse content.

1000 TWO TYPES OF HUMAN PANCREATIC ACINAR CELLS DEFINED BY SINGLE-CELL RNA SEQUENCING

2020 ◽  
Vol 158 (6) ◽  
pp. S-199-S-200
Author(s):  
Brandon M. Blobner ◽  
Jami Saloman ◽  
Celeste Shelton ◽  
Robert Lafyatis ◽  
Martin Wijkstrom ◽  
...  
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells ◽  
Single Cell Rna Sequencing

Dietary virgin olive oil enhances secretagogue-evoked calcium signaling in rat pancreatic acinar cells

Nutrition ◽  
2004 ◽  
Vol 20 (6) ◽  
pp. 536-541 ◽  
Author(s):  
María A Martínez ◽  
Ana I Lajas ◽  
María D Yago ◽  
Pedro C Redondo ◽  
María P Granados ◽  
...  
Keyword(s):  
Calcium Signaling ◽  
Olive Oil ◽  
Virgin Olive Oil ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells

scholarly journals TRPM4 links calcium signaling to membrane potential in pancreatic acinar cells

2021 ◽  
pp. 101015
Author(s):  
Gyula Diszházi ◽  
Zsuzsanna É. Magyar ◽  
Erika Lisztes ◽  
Edit Tóth-Molnár ◽  
Péter P. Nánási ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Calcium Signaling ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells

Agonist-Stimulated Pathways of Calcium Signaling in Pancreatic Acinar Cells

1999 ◽  
Vol 380 (7-8) ◽  
Author(s):  
I. Schulz ◽  
E. Krause ◽  
A. González ◽  
A. Göbel ◽  
L. Sternfeld ◽  
...  
Keyword(s):  
Calcium Signaling ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells

scholarly journals Calcium signaling of pancreatic acinar cells in the pathogenesis of pancreatitis

2014 ◽  
Vol 20 (43) ◽  
pp. 16146 ◽  
Author(s):  
Jun Li ◽  
Rui Zhou ◽  
Jian Zhang ◽  
Zong-Fang Li
Keyword(s):  
Calcium Signaling ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells
Sign in / Sign up

Export Citation Format

Share Document