Agonist-Stimulated Pathways of Calcium Signaling in Pancreatic Acinar Cells

1999 ◽  
Vol 380 (7-8) ◽  
Author(s):  
I. Schulz ◽  
E. Krause ◽  
A. González ◽  
A. Göbel ◽  
L. Sternfeld ◽  
...  
Keyword(s):  
Calcium Signaling ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells

Oscillatory mode of calcium signaling in rat pancreatic acinar cells

1990 ◽  
Vol 258 (1) ◽  
pp. C147-C155 ◽  
Author(s):  
Y. Tsunoda ◽  
E. L. Stuenkel ◽  
J. A. Williams
Keyword(s):  
Calcium Signaling ◽  
Calcium Concentration ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells ◽  
Free Calcium ◽  
Subsequent Increase ◽  
High K ◽  
Free Calcium Concentration ◽  
High Concentrations ◽  
Sustained Increase

Cytoplasmic free calcium concentration ((Ca2+]i) was evaluated by dual-wavelength microspectrofluorometry of fura-2-loaded individual rat pancreatic acinar cells. Resting [Ca2+]i in unstimulated acini was 94.1 +/- 4.1 nM. Stimulation with high concentrations of cholecystokinin (CCK, 100 pM to 1 nM) led to an immediate rise in [Ca2+]i to 400-1,000 nM followed by a fall within 2-5 min to a plateau only slightly above the prestimulation level. Lower and more physiological concentrations of CCK (1-30 pM), after a latent period of 60-90 s, induced a smaller sustained increase in [Ca2+]i (30-40 nM) with superimposed repetitive transient [Ca2+]i spikes. These oscillations averaged 120-150 nM in amplitude, occurred at a frequency which averaged 1.5 times/min, and were maintained as long as the stimulus was applied. Similar [Ca2+]i oscillations were observed when acini were stimulated with submaximal concentrations of carbamylcholine (0.1-1 microM) and neuromedin C (0.1-1 nM). Intracellular Ca2+ stores were not depleted during [Ca2+] oscillations, since a subsequent increase to 1 nM CCK led to an immediate rise in [Ca2+]i indistinguishable from the response of cells initially stimulated at this concentration. Although extracellular Ca2+ was required for maintenance of frequency of the spikes, the major source of Ca2+ utilized for oscillations was intracellular, since elimination of medium Ca2+ or Ca2+ entry blockade with lanthanum failed to inhibit oscillations. Vasoactive intestinal polypeptide (10 nM) and high K+ (50 mM) did not affect [Ca2+]i oscillations. Antimycin (10 microM), which depletes cytoplasmic ATP, increased basal [Ca2+]i and inhibited the oscillations.(ABSTRACT TRUNCATED AT 250 WORDS)

Dietary virgin olive oil enhances secretagogue-evoked calcium signaling in rat pancreatic acinar cells

Nutrition ◽  
2004 ◽  
Vol 20 (6) ◽  
pp. 536-541 ◽  
Author(s):  
María A Martínez ◽  
Ana I Lajas ◽  
María D Yago ◽  
Pedro C Redondo ◽  
María P Granados ◽  
...  
Keyword(s):  
Calcium Signaling ◽  
Olive Oil ◽  
Virgin Olive Oil ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells

scholarly journals TRPM4 links calcium signaling to membrane potential in pancreatic acinar cells

2021 ◽  
pp. 101015
Author(s):  
Gyula Diszházi ◽  
Zsuzsanna É. Magyar ◽  
Erika Lisztes ◽  
Edit Tóth-Molnár ◽  
Péter P. Nánási ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Calcium Signaling ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells

scholarly journals Calcium signaling of pancreatic acinar cells in the pathogenesis of pancreatitis

2014 ◽  
Vol 20 (43) ◽  
pp. 16146 ◽  
Author(s):  
Jun Li ◽  
Rui Zhou ◽  
Jian Zhang ◽  
Zong-Fang Li
Keyword(s):  
Calcium Signaling ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells

Visualization of CCK-evoked exocytic events in rat pancreatic acinar cells

2001 ◽  
Vol 120 (5) ◽  
pp. A24-A24
Author(s):  
H GAISANO ◽  
L TANG ◽  
L SHEU ◽  
W TRIMBLE
Keyword(s):  
Acinar Cells ◽  
Pancreatic Acinar Cells

Effects of oncogenic K-ras on pancreatic acinar cells In vitro

2001 ◽  
Vol 120 (5) ◽  
pp. A722-A722
Author(s):  
Y BI ◽  
C LOGSDON
Keyword(s):  
Acinar Cells ◽  
Pancreatic Acinar Cells
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