Maslinic acid added to the diet increases growth and protein-turnover rates in the white muscle of rainbow trout (Oncorhynchus mykiss)

2008 ◽  
Vol 147 (2) ◽  
pp. 158-167 ◽  
Author(s):  
Mónica Fernández-Navarro ◽  
Juan Peragón ◽  
Victoria Amores ◽  
Manuel De La Higuera ◽  
José Antonio Lupiáñez
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Protein Turnover ◽  
White Muscle ◽  
Turnover Rates ◽  
Maslinic Acid ◽  
Rainbow Trout Oncorhynchus Mykiss

Carbohydrates affect protein-turnover rates, growth, and nucleic acid content in the white muscle of rainbow trout (Oncorhynchus mykiss)

Aquaculture ◽  
1999 ◽  
Vol 179 (1-4) ◽  
pp. 425-437 ◽  
Author(s):  
Juan Peragón ◽  
Juan B Barroso ◽  
Leticia Garcı́a-Salguero ◽  
Manuel de la Higuera ◽  
José A Lupiáñez
Keyword(s):  
Rainbow Trout ◽  
Nucleic Acid ◽  
Oncorhynchus Mykiss ◽  
Acid Content ◽  
Protein Turnover ◽  
White Muscle ◽  
Nucleic Acid Content ◽  
Turnover Rates ◽  
Rainbow Trout Oncorhynchus Mykiss

Relationship between growth and protein turnover rates and nucleic acids in the liver of rainbow trout (Oncorhynchus mykiss) during development

1998 ◽  
Vol 55 (3) ◽  
pp. 649-657 ◽  
Author(s):  
Juan Peragón ◽  
Juan B Barroso ◽  
Manuel de la Higuera ◽  
José A Lupiáñez
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Protein Turnover ◽  
Whole Body ◽  
Turnover Rates ◽  
Retention Efficiency ◽  
Liver Growth ◽  
Protein Retention ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Total Dna

We have studied the nucleic acid concentrations and protein turnover rates, fractional protein synthesis (KS), degradation (KD), and accumulation (KG) rates, synthesis capacity (CS), synthesis efficiency (KRNA), synthesis per DNA unit (KDNA), and protein retention efficiency (PRE) in the liver of rainbow trout (Oncorhynchus mykiss) at different stages of development (fingerling: 7 g; juvenile: 45, 65, and 170 g; adult: 420 g; represented by 14-, 24-, 28-, 40-, and 96-week-old fish). Our results show that liver growth is characterized by linear increases in both total DNA (reflecting hyperplasia) and the protein:DNA ratio (reflecting hypertrophy); hyperplasia was more pronounced than hypertrophy. Development was accompanied by a significant decrease in KS, while KGdid not change significantly. There was a concomitant fall in RNA concentration measured as milligrams per gram of protein (CS) and as milligrams per gram of liver, while KRNAonly decreased slightly. The alterations in KSwere thus probably due to a decrease in CS. The pronounced fall in KStogether with the slight increase in the protein:DNA ratio may be responsible for the decline in KDNA. PRE increased concomitantly with an increase in whole-body weight.

RNA/DNA ratios in white muscle as estimates of growth in rainbow trout held at different temperatures

1990 ◽  
Vol 68 (7) ◽  
pp. 1494-1498 ◽  
Author(s):  
Moira M. Ferguson ◽  
Roy G. Danzmann
Keyword(s):  
Growth Rate ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
White Muscle ◽  
Specific Growth ◽  
Fish Size ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Different Temperatures ◽  
Specific Growth Rates ◽  
Rna And Dna

The concentrations of RNA, DNA, and protein in white muscle from 240 uniquely tagged rainbow trout (Oncorhynchus mykiss) held at three temperatures (5, 8 (control), and 11 °C) were measured. Both RNA and RNA/DNA ratios were better predictors of recent length- and weight-specific growth rates than they were of absolute fish size. Furthermore, RNA concentrations were better predictors of growth than RNA/DNA ratios. The strength of the regression between either RNA/DNA ratio or RNA and growth rate did not differ consistently among temperatures. Fish reared at warmer temperatures had lower concentrations of RNA for both a given growth rate and a given DNA concentration compared with cold-reared trout. Warm-reared fish also had lower concentrations of DNA and higher protein/DNA ratios than cold-reared trout when fish size was standardized. The concomitant decrease in both RNA and DNA concentrations resulted in marginally lower RNA/DNA ratios in warm-reared fish.

Effects of chronic sublethal ammonia and a simulated summer global warming scenario: protein synthesis in juvenile rainbow trout (Oncorhynchus mykiss)

1998 ◽  
Vol 55 (6) ◽  
pp. 1534-1544 ◽  
Author(s):  
Scott D Reid ◽  
T K Linton ◽  
J J Dockray ◽  
D G McDonald ◽  
C M Wood
Keyword(s):  
Protein Synthesis ◽  
Global Warming ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Protein Turnover ◽  
Warming Scenario ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Global Warming Scenario ◽  
Protein Synthesis And Degradation ◽  
Synthesis And Degradation

Protein synthesis, net accretion, and degradation in liver, gill, and white muscle and ribosomal translational efficiency and protein synthesis capacity in liver and gill were measured using a flooding dose of [3H]phenylalanine in juvenile rainbow trout (Oncorhynchus mykiss). The fish were chronically exposed (90 days) in hardwater to the presence or absence of sublethal ammonia (70 µmol total ammonia ·L-1) alone or in combination with a 2°C elevation in the normal temperature profile over the months of June-September 1993 (ambient temperature range 13-22°C). Chronic sublethal exposure to ammonia had little impact on gill protein synthesis and degradation (protein turnover) and even less in muscle. However, in the liver, both protein synthesis and degradation were stimulated following 60 days of the sublethal ammonia exposure. The 2°C elevation in temperature resulted in a slight increase in protein turnover in both gills and liver. However, during the period of peak water temperature, the 2°C elevation in temperature inhibited protein dynamics in these tissues. Overall, elevated environmental ammonia in combination with a summer global warming scenario would challenge the ability of fish to adapt to alterations in the quality of their environment, most notably during periods of peak temperatures.

Metabolic effects of the expression of a phosphoglucomutase locus in the liver of rainbow trout

1990 ◽  
Vol 68 (7) ◽  
pp. 1499-1504
Author(s):  
Moira M. Ferguson ◽  
Roy G. Danzmann ◽  
Fred W. Allendorf ◽  
Kathy L. Knudsen
Keyword(s):  
Oxygen Consumption ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
White Muscle ◽  
Metabolic Effects ◽  
Parallel Analysis ◽  
Separate Experiment ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Consumption Rates ◽  
Sib Families

We examined the lengths, weights, condition factors, and hepatosomatic indices of juvenile rainbow trout (Oncorhynchus mykiss) from four full-sib families, each segregating at the temporal regulatory locus Pgm1-t, and the concentrations of RNA, DNA, and protein in their livers and white muscle. In three families, fish with phosphoglucomutase-1 (PGM1) activity in liver (Pgm1-t(b) fish) are significantly longer than their full-sibs lacking activity for liver PGM1 (Pgm1-t(a) fish). Hepatosomatic indices tend to be higher in the Pgm1-t(b) fish than in their Pgm1-t(a) siblings. RNA/DNA ratios in the liver of Pgm1-t(b) fish are significantly higher than those of Pgm1-t(a) fish in two families and marginal in a third. However, no significant differences were detected in a parallel analysis of nucleic acids and protein in white muscle, where PGM1 is expressed in all fish. In a separate experiment, Pgm1-t(b) fish were significantly heavier in all five families, had significantly higher condition factors in two families, and had marginally lower standardized oxygen consumption rates in three families.

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