Relationship between growth and protein turnover rates and nucleic acids in the liver of rainbow trout (Oncorhynchus mykiss) during development

1998 ◽  
Vol 55 (3) ◽  
pp. 649-657 ◽  
Author(s):  
Juan Peragón ◽  
Juan B Barroso ◽  
Manuel de la Higuera ◽  
José A Lupiáñez
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Protein Turnover ◽  
Whole Body ◽  
Turnover Rates ◽  
Retention Efficiency ◽  
Liver Growth ◽  
Protein Retention ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Total Dna

We have studied the nucleic acid concentrations and protein turnover rates, fractional protein synthesis (KS), degradation (KD), and accumulation (KG) rates, synthesis capacity (CS), synthesis efficiency (KRNA), synthesis per DNA unit (KDNA), and protein retention efficiency (PRE) in the liver of rainbow trout (Oncorhynchus mykiss) at different stages of development (fingerling: 7 g; juvenile: 45, 65, and 170 g; adult: 420 g; represented by 14-, 24-, 28-, 40-, and 96-week-old fish). Our results show that liver growth is characterized by linear increases in both total DNA (reflecting hyperplasia) and the protein:DNA ratio (reflecting hypertrophy); hyperplasia was more pronounced than hypertrophy. Development was accompanied by a significant decrease in KS, while KGdid not change significantly. There was a concomitant fall in RNA concentration measured as milligrams per gram of protein (CS) and as milligrams per gram of liver, while KRNAonly decreased slightly. The alterations in KSwere thus probably due to a decrease in CS. The pronounced fall in KStogether with the slight increase in the protein:DNA ratio may be responsible for the decline in KDNA. PRE increased concomitantly with an increase in whole-body weight.

Carbohydrates affect protein-turnover rates, growth, and nucleic acid content in the white muscle of rainbow trout (Oncorhynchus mykiss)

Aquaculture ◽  
1999 ◽  
Vol 179 (1-4) ◽  
pp. 425-437 ◽  
Author(s):  
Juan Peragón ◽  
Juan B Barroso ◽  
Leticia Garcı́a-Salguero ◽  
Manuel de la Higuera ◽  
José A Lupiáñez
Keyword(s):  
Rainbow Trout ◽  
Nucleic Acid ◽  
Oncorhynchus Mykiss ◽  
Acid Content ◽  
Protein Turnover ◽  
White Muscle ◽  
Nucleic Acid Content ◽  
Turnover Rates ◽  
Rainbow Trout Oncorhynchus Mykiss

Composition and Mechanics of Routine Swimming of Rainbow Trout, Oncorhynchus mykiss

1991 ◽  
Vol 48 (4) ◽  
pp. 583-590 ◽  
Author(s):  
Paul W. Webb
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Correction Factor ◽  
Swimming Speed ◽  
Angular Acceleration ◽  
Force Balance ◽  
Whole Body ◽  
Strongly Correlated ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Tail Beat

Routine swimming encompasses all volitional motions of fish. It is usually assumed to be quasi-steady, i.e. routine swimming is mechanically equivalent to steady swimming. Routine swimming of rainbow trout, Oncorhynchus mykiss, was dominated by unsteady motions of linear and centripetal (angular) acceleration. Constant-speed swimming was rare. Mean speeds and acceleration rates were small. Tail-beat frequencies were nevertheless strongly correlated with mean swimming speed, but increased more rapidly with increasing speed in routine swimming than in steady swimming. Tail-beat amplitudes and propulsive wavelengths were similar to values seen in steady swimming. The composition of routine swimming and analysis of the force balance showed that routine swimming was not quasi-steady. Therefore, forces and rates of working should be estimated from a complete description of whole-body deformation. This is impractical. Drag dominated resistance in routine swimming, such that average thrust (= resistance) may be computed from mean speed and/or averaged kinematic variables for the trailing edge with a correction factor of approximately 3. Analysis of routine swimming may permit comparisons among a wider range of vertebrates than possible with commonly used methods.

Acute and Chronic Toxicity of Waterborne Arsenite to Rainbow Trout (Oncorhynchus mykiss)

1994 ◽  
Vol 51 (2) ◽  
pp. 372-380 ◽  
Author(s):  
M. G. Rankin ◽  
D. G. Dixon
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Chronic Toxicity ◽  
Plasma Lipid ◽  
Free Water ◽  
Gallbladder Wall ◽  
Whole Body ◽  
Growth Study ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Wet Weight

The 144-h LC50 (95% fiducial limits) of arsenite for 4.5-g rainbow trout (Oncorhynchus mykiss) was 18.5 (17.9–19.1) mg∙L−1. In a pair-fed growth study which exposed trout to 0.0, 0.76, 2.48, or 9.64 mg arsenite∙L−1 over 17 wk, growth was significantly reduced (by 55%) only at 9.64 mg∙L−1. The reduction was attributable to both reduced appetite (primarily) and direct metabolic impact (marginally). Fish at 9.64 mg∙L−1 suffered 10% mortality, usually associated with necrotic erosion of the mandibular and olfactory regions of the head. All fish exposed to 9.64 mg∙L−1 showed inflammation of the gallbladder wall, a lesion absent at lower exposure concentrations. There were no arsenite impacts on hepato- and splenosomatic index, hematocrit, hemoglobin, total plasma lipid, cholesterol, and protein or brain concentrations of norepinephrine, dopamine, and serotonin. Exposure to 0.0, 0.76, 2.48, and 9.64 mg arsenite∙L−1 for 26 wk resulted in mean (SE) equilibrium whole-body As concentrations of 0.3 (0.02), 0.2 (0.02), 0.4 (0.10), and 1.7 (0.40) μg As∙g wet weight−1, respectively, No depuration below these concentrations occurred during a 12-d period in arsenite-free water. The threshold of chronic toxicity was estimated to be 4.9 mg∙L−1.

Diminished social status affects ionoregulation at the gills and kidney in rainbow trout (Oncorhynchus mykiss)

2004 ◽  
Vol 61 (4) ◽  
pp. 618-626 ◽  
Author(s):  
Katherine A Sloman ◽  
Graham R Scott ◽  
D Gordon McDonald ◽  
Chris M Wood
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Social Status ◽  
Metabolic Cost ◽  
Whole Body ◽  
Sodium Reabsorption ◽  
Plasma Sodium ◽  
Sodium Efflux ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Uptake Rates

Competition for social status can result in physiological differences between individuals, including differences in ionoregulatory ability. Subordinate rainbow trout (Oncorhynchus mykiss) had two-fold higher uptake rates of sodium across the gill and two-fold higher whole-body sodium efflux rates than the dominant fish with which they were paired. Sodium efflux was then divided into branchial and renal components, both of which were higher in subordinates. Branchial sodium efflux accounted for 95%–98% of sodium loss. Plasma sodium concentrations were more variable, although not significantly different, in subordinate fish, suggesting that the increased loss of sodium in these trout is compensated for by an increase in uptake rates. Urine flow rates and plasma cortisol concentrations were higher in subordinate fish, but there was no difference in glomerular filtration rate between dominants and subordinates. Renal sodium reabsorption was significantly reduced in subordinates. In summary, the ionoregulation of subordinate individuals was altered, most likely occurring as a result of stress-induced changes in gill permeability, resulting in a higher throughput of water and increased branchial sodium efflux. These changes in ionoregulatory ability have many physiological implications, including the increased susceptibility of subordinates to ionoregulatory challenges and an increased metabolic cost of ionoregulation.

Conspecific skin extracts elicit antipredator responses in juvenile rainbow trout (Oncorhynchus mykiss)

1997 ◽  
Vol 75 (11) ◽  
pp. 1916-1922 ◽  
Author(s):  
Grant E. Brown ◽  
R. Jan F. Smith
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Whole Body ◽  
Antipredator Behaviour ◽  
Skin Extract ◽  
Distilled Water ◽  
Juvenile Rainbow Trout ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Antipredator Responses ◽  
Body Extract

We investigated, under laboratory conditions, the presence of chemical alarm signals in juvenile rainbow trout (Oncorhynchus mykiss). In an initial experiment, we exposed trout to a whole-body extract from conspecifics or a distilled-water control. When exposed to whole-body extract, trout significantly (i) decreased time spent swimming, (ii) increased time taken to resume foraging, and (iii) decreased the number of food items eaten. These data indicate a significant chemically mediated antipredator response. A second experiment was conducted to determine (i) if this is a generalized response to injured fish or a specific response to injured conspecifics, and (ii) if the chemical signal is localized in the skin. We exposed juvenile trout to one of three chemical stimuli: (1) trout skin extract, (2) trout body extract, or (3) swordtail (Xiphophorus helleri) skin extract. Significant antipredator responses were observed in trout exposed to conspecific skin extract, but responses of those exposed to conspecific body extract or swordtail skin extract did not differ from those of distilled-water controls. These data strongly suggest that juvenile rainbow trout possess a chemical alarm signal, localized in the skin, that elicits antipredator behaviour when detected.by conspecifics.

Comparison of phosphorus retention efficiency between rainbow trout (Oncorhynchus mykiss) fed a commercial diet and a low fish meal based diet

Aquaculture ◽  
2003 ◽  
Vol 224 (1-4) ◽  
pp. 271-282 ◽  
Author(s):  
Shuichi Satoh ◽  
Adrián Hernández ◽  
Takatoshi Tokoro ◽  
Yosuke Morishita ◽  
Viswanath Kiron ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Fish Meal ◽  
Phosphorus Retention ◽  
Retention Efficiency ◽  
Commercial Diet ◽  
Rainbow Trout Oncorhynchus Mykiss
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