scholarly journals Lactate efflux from sarcolemmal vesicles isolated from rainbow trout Oncorhynchus mykiss white muscle is via simple diffusion

2003 ◽  
Vol 206 (3) ◽  
pp. 543-549 ◽  
Author(s):  
R. L. Sharpe
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
White Muscle ◽  
Simple Diffusion ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Sarcolemmal Vesicles

RNA/DNA ratios in white muscle as estimates of growth in rainbow trout held at different temperatures

1990 ◽  
Vol 68 (7) ◽  
pp. 1494-1498 ◽  
Author(s):  
Moira M. Ferguson ◽  
Roy G. Danzmann
Keyword(s):  
Growth Rate ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
White Muscle ◽  
Specific Growth ◽  
Fish Size ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Different Temperatures ◽  
Specific Growth Rates ◽  
Rna And Dna

The concentrations of RNA, DNA, and protein in white muscle from 240 uniquely tagged rainbow trout (Oncorhynchus mykiss) held at three temperatures (5, 8 (control), and 11 °C) were measured. Both RNA and RNA/DNA ratios were better predictors of recent length- and weight-specific growth rates than they were of absolute fish size. Furthermore, RNA concentrations were better predictors of growth than RNA/DNA ratios. The strength of the regression between either RNA/DNA ratio or RNA and growth rate did not differ consistently among temperatures. Fish reared at warmer temperatures had lower concentrations of RNA for both a given growth rate and a given DNA concentration compared with cold-reared trout. Warm-reared fish also had lower concentrations of DNA and higher protein/DNA ratios than cold-reared trout when fish size was standardized. The concomitant decrease in both RNA and DNA concentrations resulted in marginally lower RNA/DNA ratios in warm-reared fish.

Lactate transport across sarcolemmal vesicles isolated from rainbow trout white muscle

1999 ◽  
Vol 202 (16) ◽  
pp. 2167-2175 ◽  
Author(s):  
K. Laberee ◽  
C.L. Milligan
Keyword(s):  
Rainbow Trout ◽  
White Muscle ◽  
High Capacity ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Transport Inhibitor ◽  
Monocarboxylate Transport ◽  
Disulphonic Acid ◽  
Lactate Uptake ◽  
High Concentrations ◽  
Sarcolemmal Vesicles

Rainbow trout (Oncorhynchus mykiss) retain the majority of lactate produced during exhaustive exercise within white muscle. Previous studies have suggested that this retention is partially via a re-uptake of released lactate. The purpose of this work was to study lactate uptake using trout white muscle sarcolemmal vesicles. Lactate uptake by trout white muscle is partially through a low-affinity, high-capacity carrier (apparent K(m)=55.6 mmol l(−)(1) and V(max)=44.5 nmol mg(−)(1)protein min(−)(1)). At high concentrations (20 and 50 mmol l(−)(1)), pyruvate partially (up to 39 %) inhibited lactate uptake, suggesting the involvement of a monocarboxylate carrier. The anion transport inhibitor 4-acetoamido-4′-isothiocyanstilbene-2,2′-disulphonic acid (SITS) and the monocarboxylate transport inhibitor (α)-cyano-4-hydroxycinnamate (CHC) stimulated apparent lactate uptake. The model developed suggests that lactate is taken up by the vesicles, at least in part by a pyruvate-sensitive monocarboxylate carrier, and that its subsequent efflux is inhibited by SITS and CHC, suggesting that lactate export from trout white muscle is also carrier-mediated.

Metabolic effects of the expression of a phosphoglucomutase locus in the liver of rainbow trout

1990 ◽  
Vol 68 (7) ◽  
pp. 1499-1504
Author(s):  
Moira M. Ferguson ◽  
Roy G. Danzmann ◽  
Fred W. Allendorf ◽  
Kathy L. Knudsen
Keyword(s):  
Oxygen Consumption ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
White Muscle ◽  
Metabolic Effects ◽  
Parallel Analysis ◽  
Separate Experiment ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Consumption Rates ◽  
Sib Families

We examined the lengths, weights, condition factors, and hepatosomatic indices of juvenile rainbow trout (Oncorhynchus mykiss) from four full-sib families, each segregating at the temporal regulatory locus Pgm1-t, and the concentrations of RNA, DNA, and protein in their livers and white muscle. In three families, fish with phosphoglucomutase-1 (PGM1) activity in liver (Pgm1-t(b) fish) are significantly longer than their full-sibs lacking activity for liver PGM1 (Pgm1-t(a) fish). Hepatosomatic indices tend to be higher in the Pgm1-t(b) fish than in their Pgm1-t(a) siblings. RNA/DNA ratios in the liver of Pgm1-t(b) fish are significantly higher than those of Pgm1-t(a) fish in two families and marginal in a third. However, no significant differences were detected in a parallel analysis of nucleic acids and protein in white muscle, where PGM1 is expressed in all fish. In a separate experiment, Pgm1-t(b) fish were significantly heavier in all five families, had significantly higher condition factors in two families, and had marginally lower standardized oxygen consumption rates in three families.

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