Protein kinase A activity and protein phosphorylation in the haemocytes of immune-challenged Galleria mellonella larvae

2007 ◽  
Vol 148 (1) ◽  
pp. 74-83 ◽  
Author(s):  
Małgorzata Cytryńska ◽  
Agnieszka Zdybicka-Barabas ◽  
Teresa Jakubowicz
Keyword(s):  
Protein Kinase ◽  
Protein Phosphorylation ◽  
Protein Kinase A ◽  
Galleria Mellonella ◽  
Kinase A

scholarly journals The involvement of protein kinase A in the immune response of Galleria mellonella larvae to bacteria.

2007 ◽  
Vol 54 (1) ◽  
pp. 167-174 ◽  
Author(s):  
Małgorzata Cytryńska ◽  
Agnieszka Zdybicka-Barabas ◽  
Teresa Jakubowicz
Keyword(s):  
Immune Response ◽  
Enzyme Activity ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Galleria Mellonella ◽  
Fat Body ◽  
Immune Challenge ◽  
Gram Negative ◽  
E Coli ◽  
Kinase A

The role of protein kinase A (PKA) in the humoral immune response of the greater wax moth Galleria mellonella larvae to live gram-positive bacteria Micrococcus lysodeikticus and gram-negative bacteria Escherichia coli was investigated. The immune challenge of larvae with both kinds of bacteria caused an increase in fat body PKA activity depending on the injected bacteria. Gram-positive M. lysodeikticus was a much better inducer of the enzyme activity than gram-negative E. coli. The PKA activity was increased about 2.5-fold and 1.5-fold, after M. lysodeikticus and E. coli injection, respectively. The in vivo inhibition of the enzyme activity by a cell permeable selective PKA inhibitor, Rp-8-Br-cAMPS, was correlated with considerable changes of fat body lysozyme content and hemolymph antimicrobial activity in bacteria-challenged insects. The kinetics of changes were different and dependent on the bacteria used for the immune challenge of G. mellonella larvae.

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