Studies on the role of protein kinase A in humoral immune response of Galleria mellonella larvae

2006 ◽  
Vol 52 (7) ◽  
pp. 744-753 ◽  
Author(s):  
Małgorzata Cytryńska ◽  
Agnieszka Zdybicka-Barabas ◽  
Teresa Jakubowicz
Keyword(s):  
Immune Response ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Humoral Immune Response ◽  
Galleria Mellonella ◽  
Humoral Immune ◽  
Kinase A

scholarly journals The involvement of protein kinase A in the immune response of Galleria mellonella larvae to bacteria.

2007 ◽  
Vol 54 (1) ◽  
pp. 167-174 ◽  
Author(s):  
Małgorzata Cytryńska ◽  
Agnieszka Zdybicka-Barabas ◽  
Teresa Jakubowicz
Keyword(s):  
Immune Response ◽  
Enzyme Activity ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Galleria Mellonella ◽  
Fat Body ◽  
Immune Challenge ◽  
Gram Negative ◽  
E Coli ◽  
Kinase A

The role of protein kinase A (PKA) in the humoral immune response of the greater wax moth Galleria mellonella larvae to live gram-positive bacteria Micrococcus lysodeikticus and gram-negative bacteria Escherichia coli was investigated. The immune challenge of larvae with both kinds of bacteria caused an increase in fat body PKA activity depending on the injected bacteria. Gram-positive M. lysodeikticus was a much better inducer of the enzyme activity than gram-negative E. coli. The PKA activity was increased about 2.5-fold and 1.5-fold, after M. lysodeikticus and E. coli injection, respectively. The in vivo inhibition of the enzyme activity by a cell permeable selective PKA inhibitor, Rp-8-Br-cAMPS, was correlated with considerable changes of fat body lysozyme content and hemolymph antimicrobial activity in bacteria-challenged insects. The kinetics of changes were different and dependent on the bacteria used for the immune challenge of G. mellonella larvae.

Role of Yersinia pseudotuberculosis outer proteins (Yops) in murine humoral immune response

2009 ◽  
Vol 54 (3) ◽  
pp. 239-245 ◽  
Author(s):  
J. M. L. Maia ◽  
L. G. S. Monnazzi ◽  
B. M. M. Medeiros
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Yersinia Pseudotuberculosis ◽  
Humoral Immune

Regulation of arginine vasopressin mRNA in rat fetal hypothalamic cell culture. Role of protein kinases and glucocorticoids

1993 ◽  
Vol 10 (1) ◽  
pp. 51-57 ◽  
Author(s):  
S-B Hu ◽  
L A Tannahill ◽  
S L Lightman
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Mrna Expression ◽  
Protein Kinase A ◽  
Arginine Vasopressin ◽  
In Situ Hybridization Histochemistry ◽  
Kinase C ◽  
Kinase A

ABSTRACT Studies have been performed to investigate the regulation of arginine vasopressin (AVP) mRNA expression in fetal hypothalamic cultures. AVP mRNA-positive neurones were identified by in-situ hybridization histochemistry, and changes in mRNA expression were quantitated by nuclease protection assay. Both protein kinase C and protein kinase A activators increased the expression of AVP mRNA, in contrast to dexamethasone, which inhibited the responses to both protein kinase C and protein kinase A activation.

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