Activation of apoptosis by ethyl acetate fraction of ethanol extract of Dianthus superbus in HepG2 cell line

2012 ◽  
Vol 36 (1) ◽  
pp. e40-e45 ◽  
Author(s):  
Jian-Qing Yu ◽  
Yan Yin ◽  
Jia-Chuan Lei ◽  
Xiu-Qiao Zhang ◽  
Wei Chen ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hepg2 Cell Line

scholarly journals ANTICANCER ACTIVITY OF ARGEMONE MEXICANA L (FLOWERS) AGAINST HUMAN LIVER CANCER (HEPG2) CELL LINE

2017 ◽  
Vol 10 (10) ◽  
pp. 351
Author(s):  
Prabhakaran D ◽  
Senthamilselvi Mm ◽  
Rajeshkanna A
Keyword(s):  
Liver Cancer ◽  
Ethyl Acetate ◽  
Anticancer Activity ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Ethyl Acetate Fraction ◽  
Hepatoma Cell Line ◽  
Hepg2 Cell Line ◽  
Argemone Mexicana ◽  
Flower Extract

  Objective: To explore the anticancer activity of the flowers of Argemone mexicana L. against the human hepatoma cell line (HepG2).Methods: In vitro anticancer activity was carried out to screen cytotoxicity effectiveness of the solid obtained from ethyl acetate fraction of A. mexicana L. flower extract at different concentrations against the HepG2 cell line. The MTT (methylthiazolyl diphenyl- tetrazolium bromide) assay for cell viability and markers is expected to confirm the cytotoxicity.Result: Ethyl acetate fraction from the flower extract of A. mexicana L. was tested for its anticancer activity against HepG2 cell lines (liver cancer) at various concentrations by MTT assay. It was confirmed that the IC50 value of this sample was 72±1.7 μg/ml against liver cancer HepG2 cell line.Conclusions: A. mexicana L. is a potential plant with anticancer activity. The isolation of the pure compounds and determination of the structure of individual compounds will be further performed.

scholarly journals Cytotoxic activities of Ganoderma lucidum ethanol extract against HepG2 cell line

2016 ◽  
Vol 11 (3) ◽  
pp. 632
Author(s):  
Kannan Mohan ◽  
Muthusamy Padmanaban ◽  
Venkatachalam Uthayakumar ◽  
Ramachandran Chandrasekhar ◽  
Thirunavukkarasu Muralisankar
Keyword(s):  
Cell Line ◽  
Hepg2 Cell ◽  
Ganoderma Lucidum ◽  
Ethanol Extract ◽  
Cytotoxic Activities ◽  
Hepg2 Cell Line

scholarly journals Hepatoprotective Potential of Tecomella undulata Bark on Paracetamol and CCL4 Induced Hepatotoxicity in Rats: Invitro Analysis

2021 ◽  
pp. 307-322
Author(s):  
Prasoon Kumar Saxena ◽  
Deepak Nanda ◽  
Ritu Gupta
Keyword(s):  
Alkaline Phosphatase ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Reduced Glutathione ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Serum Marker ◽  
Hepg2 Cell Line ◽  
Marker Enzymes ◽  
Tecomella Undulata

In this study the T. undulata bark was tested for its hepatoprotection against paracetamol (PCM) induced hepatic damage and Carbon tetra chloride (CCl4) induced hepatotoxicity. The Invitro study was performed on HepG2 cell line .The Levels of serum marker enzymes i.e. AST, ALT (aminotransferases), ALP (alkaline phosphatase), GSH (reduced glutathione) and MDA (Malondialdehyde) in 70% Ethanol treated rats were monitored, respectively. The 70% Ethanol extract gave promising results as studied in detail. The Present study showed that the 70 % ethanolic extract of bark of T.undulata apparently revive the physiological integrity of hepatocytes. Thus the present study demonstrated the Hepatoprotective property of Tecomella undulate Bark.

Antioxidant Activity, Inhibition α-Glucosidase of Ethanol Extract of Strychnos nitida G. Don and Identification of Active Compounds

2019 ◽  
Vol 5 (3) ◽  
pp. 11-20
Author(s):  
Stefani Dhale Rale ◽  
Hasim Hasim ◽  
Syamsul Falah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Inhibition Activity ◽  
Active Compounds ◽  
Activity Inhibition ◽  
Activity Test ◽  
Ic50 Value ◽  
Treatment Of Diabetes

This study aims to find the treatment of diabetes using natural materials by exploring plants in the province of East Nusa Tenggara. his research was conducted out by extracting the Strychnos nitida G.Don stem using a method of maceration by ethanol 70%. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Simplicia from maceration and fractionation results were then tested for antioxidant activity, α-glucosidase inhibition activity and identification of active compounds. The results showed that ethyl acetate fraction had the lowest IC50 value of 86.83 μg / ml. Results of the α-glucosidase activity test showed that ethyl acetate fraction and n-heksan fraction at 900 ppm had the highest percentage of inhibition of 34.23% and 33.89%. Identification using LCMS/MS method showed that ethyl acetate fraction consist of Benzenemethamine, N, N-dioctyl- as an antioxidantcompound and compound 24-methyl-5-cholestone-hexol as an antidiabetic compound. From the results of this study, we concluded that the extract of kayu ular Strychnos nitida G.Don stem has inhibition activity toward α-glucosidase enzyme.

scholarly journals Antibacterial Activity Test of Extracts and Fractions of Cassava Leaves (Manihot esculenta Crantz) against Clinical Isolates of Staphylococcus epidermidis and Propionibacterium acnes Causing Acne

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Resmi Mustarichie ◽  
Sulistiyaningsih Sulistyaningsih ◽  
Dudi Runadi
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Clinical Isolates ◽  
Active Fraction ◽  
Cassava Leaves ◽  
Ethanol Extracts

This study is aimed at determining antibacterial activity from ethanol extracts and the most active fraction of cassava leaves against clinical isolates of Staphylococcus epidermidis and Propionibacterium acnes. Research carried out by the experimental method involved determination of plants, extraction with maceration method, fractionation with liquid-liquid extraction, antibacterial activity testing of extracts and fractions by agar diffusion method, determination of most active fraction from the extract, and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) testing of most active fraction by microdilution method. The results showed that ethanol extracts of cassava leaves had antibacterial activity against both bacteria with the most active fraction indicated by ethyl acetate. MIC values of ethyl acetate fraction against S. epidermidis were in the concentration range of 2.5%–5.0% (w/v) and against P. acnes were in the concentration range of 1.25%–2.5% (w/v). The MBC value of ethyl acetate fraction against S. epidermidis was at a concentration of 5% (w/v), while P. acnes was at a concentration of 2.5% (w/v). From the results of this study, it can be concluded that the ethanol extract of cassava leaves (Manihot esculenta Crantz) has antibacterial activity against clinical isolates of Staphylococcus epidermidis as well as on Propionibacterium acnes. The fraction with the best activity from the ethanol extract of cassava leaves to the two test bacteria was shown by ethyl acetate fraction. It is suggested that cassava leaves are possible to be developed into standardized antiacne herbal.

scholarly journals Metformin attenuates hypoxia‑induced resistance to cisplatin in the HepG2 cell line

2018 ◽  
Author(s):  
Hiromasa Fujita ◽  
Katsumi Hirose ◽  
Mariko Sato ◽  
Ichitaro Fujioka ◽  
Tamaki Fujita ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepg2 Cell ◽  
Induced Resistance ◽  
Hepg2 Cell Line
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