An in-silico proof-of-concept investigation of a combined glucose-insulin bolus quick dynamic insulin sensitivity test

2014 ◽  
Vol 10 ◽  
pp. 332-337 ◽  
Author(s):  
Paul D. Docherty ◽  
J. Geoffrey Chase
Keyword(s):  
Insulin Sensitivity ◽  
In Silico ◽  
Sensitivity Test ◽  
Proof Of Concept

Rapid insulin sensitivity test (RIST)

1998 ◽  
Vol 76 (12) ◽  
pp. 1080-1086 ◽  
Author(s):  
W Wayne Lautt ◽  
Xiaowsi Wang ◽  
Parissa Sadri ◽  
Dallas J Legare ◽  
M Paula Macedo
Keyword(s):  
Insulin Sensitivity ◽  
Operating Procedure ◽  
Insulin Action ◽  
Standard Operating Procedure ◽  
Sensitivity Test ◽  
Bolus Administration ◽  
Venous Shunt ◽  
Technical Report ◽  
Significant Difference ◽  
Standard Operating

A rapid insulin sensitivity test (RIST) was recently introduced to assess insulin action in vivo (H. Xie, L. Zhu, Y.L. Zhang, D.J. Legare, and W.W. Lautt. J. Pharmacol. Toxicol. Methods, 35: 77-82. 1996). This technical report describes the current recommended standard operating procedure for the use of the RIST in rats based upon additional experience with approximately 100 tests. We describe the manufacture and use of an arterial-venous shunt that allows rapid multiple arterial samples and intravenous administration of drugs. The RIST procedure involves determination of a stable arterial glucose baseline to define the ideal euglycemic level to be maintained following a 5-min infusion of insulin, with the RIST index being the amount of glucose required to be infused to maintain euglycemia over the test period. Insulin administration by a 5-min infusion is preferable to a 30-s bolus administration. No significant difference was determined between the use of Toronto pork-beef or human insulin. Four consecutive RISTs were carried out in the same animal over 4-5 h with no tendency for change with time. The RIST index is sufficiently sensitive and reproducible to permit establishment of insulin dose-response curves and interference of insulin action by elimination of hepatic parasympathetic nerves, using atropine. This technical report provides the current recommended standard operating procedure for the RIST.Key words: insulin, resistance, test, methodology, glucose.

scholarly journals Run-to-Run Tuning of Model Predictive Control for Type 1 Diabetes Subjects: In Silico Trial

2009 ◽  
Vol 3 (5) ◽  
pp. 1091-1098 ◽  
Author(s):  
Lalo Magni ◽  
Marco Forgione ◽  
Chiara Toffanin ◽  
Chiara Dalla Man ◽  
Boris Kovatchev ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Insulin Sensitivity ◽  
Model Predictive Control ◽  
Predictive Control ◽  
In Silico ◽  
Insulin Pump ◽  
Artificial Pancreas ◽  
Arx Model ◽  
Starting Point

Background: The technological advancements in subcutaneous continuous glucose monitoring and insulin pump delivery systems have paved the way to clinical testing of artificial pancreas devices. The experience derived by clinical trials poses technological challenges to the automatic control expert, the most notable being the large interpatient and intrapatient variability and the inherent uncertainty of patient information. Methods: A new model predictive control (MPC) glucose control system is proposed. The starting point is an MPC algorithm applied in 20 type 1 diabetes mellitus (T1DM) subjects. Three main changes are introduced: individualization of the ARX model used for prediction; synthesis of the MPC law on top of the open-loop basal/bolus therapy; and a run-to-run approach for implementing day-by-day tuning of the algorithm. In order to individualize the ARX model, a sufficiently exciting insulin profile is imposed by splitting the premeal bolus into two smaller boluses (40% and 60%) injected 30 min before and 30 min after the meal. Results: The proposed algorithm was tested on 100 virtual subjects extracted from an in silico T1DM population. The trial simulates 44 consecutive days, during which the patient receives breakfast, lunch, and dinner each day. For 10 days, meals are multiplied by a random variable uniformly distributed in [0.5, 1.5], while insulin delivery is based on nominal meals. Moreover, for 10 days, either a linear increase or decrease of insulin sensitivity (±25% of nominal value) is introduced. Conclusions: The ARX model identification procedure offers an automatic tool for patient model individualization. The run-to-run approach is an effective way to auto-tune the aggressiveness of the closed-loop control law, is robust to meal variation, and is also capable of adapting the regulator to slow parameter variations, e.g., on insulin sensitivity.

Comparison of the rapid insulin sensitivity test (RIST), the insulin tolerance test (ITT), and the hyperinsulinemic euglycemic clamp (HIEC) to measure insulin action in rats

2002 ◽  
Vol 80 (8) ◽  
pp. 811-818 ◽  
Author(s):  
Maria A.G Reid ◽  
Martin G Latour ◽  
Dallas J Legare ◽  
Na Rong ◽  
W Wayne Lautt
Keyword(s):  
Insulin Resistance ◽  
Insulin Sensitivity ◽  
Insulin Action ◽  
Initial Response ◽  
Tolerance Test ◽  
Insulin Tolerance Test ◽  
Sensitivity Test ◽  
Euglycemic Clamp ◽  
Hyperinsulinemic Euglycemic Clamp ◽  
Insulin Tolerance

The objective was to compare the ability of the rapid insulin sensitivity test (RIST), the hyperinsulinemic euglycemic clamp (HIEC), and the insulin tolerance test (ITT) to detect hepatic insulin sensitizing substance (HISS) dependent insulin action. HISS action was augmented by feeding and inhibited by fasting, blockade of hepatic nitric oxide synthase, or blockade of hepatic muscarinic cholinergic receptors. A significant correlation was found between the RIST index and ITT nadir (r2 = 0.84) but not between the glucose infusion rate of the HIEC and RIST index. There was, however, a relationship between the RIST index and the initial response during the HIEC. Use of the HIEC resulted in HISS-dependent insulin resistance in both conscious and anesthetized animals. We concluded that since the RIST and ITT were comparable in quantifying both HISS-dependent and HISS-independent insulin action, the RIST was validated against this standard. The observation that the HIEC is capable of detecting HISS action in the first rising slope of the test but not at the end of the test and that HISS release is fully blocked after the conclusion of the HIEC raises concerns about the use of the commonly used HIEC.Key words: HISS, insulin resistance, insulin sensitivity tests.

scholarly journals Peptides modulating conformational changes in secreted chaperones: From in silico design to preclinical proof of concept

2009 ◽  
Vol 106 (33) ◽  
pp. 13797-13801 ◽  
Author(s):  
Y. Kliger ◽  
O. Levy ◽  
A. Oren ◽  
H. Ashkenazy ◽  
Z. Tiran ◽  
...  
Keyword(s):  
Conformational Changes ◽  
In Silico ◽  
Proof Of Concept ◽  
In Silico Design

scholarly journals Effects of ambient temperature on glucose tolerance and insulin sensitivity test outcomes in normal and obese C57 male mice

2015 ◽  
Vol 3 (5) ◽  
pp. e12396 ◽  
Author(s):  
Anete Dudele ◽  
Gitte Marie Rasmussen ◽  
David Mayntz ◽  
Hans Malte ◽  
Sten Lund ◽  
...  
Keyword(s):  
Insulin Sensitivity ◽  
Glucose Tolerance ◽  
Ambient Temperature ◽  
Sensitivity Test ◽  
Male Mice ◽  
Test Outcomes

scholarly journals Tipless Transseptal Cannula Concept Combines Improved Hemodynamic Properties and Risk‐Reduced Placement: an In Silico Proof‐of‐Concept

2021 ◽  
Author(s):  
Kristin Hugenroth ◽  
Michael Neidlin ◽  
Ulrich M. Engelmann ◽  
Tim A.S. Kaufmann ◽  
Ulrich Steinseifer ◽  
...  
Keyword(s):  
In Silico ◽  
Proof Of Concept

scholarly journals An in silico structural approach to characterize human and rainbow trout estrogenicity of mycotoxins: Proof of concept study using zearalenone and alternariol

2020 ◽  
Vol 312 ◽  
pp. 126088 ◽  
Author(s):  
Luca Dellafiora ◽  
Isabelle P. Oswald ◽  
Jean-Lou Dorne ◽  
Gianni Galaverna ◽  
Paola Battilani ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
In Silico ◽  
Structural Approach ◽  
Proof Of Concept ◽  
Concept Study

In silico drug repositioning on F508del-CFTR: A proof-of-concept study on the AIFA library

2021 ◽  
Vol 213 ◽  
pp. 113186 ◽  
Author(s):  
Alessandro Orro ◽  
Matteo Uggeri ◽  
Marco Rusnati ◽  
Chiara Urbinati ◽  
Nicoletta Pedemonte ◽  
...  
Keyword(s):  
In Silico ◽  
Drug Repositioning ◽  
Proof Of Concept ◽  
Concept Study
Sign in / Sign up

Export Citation Format

Share Document