Intravital bone imaging by two-photon excitation microscopy to identify osteocytic osteolysis in vivo

Bone ◽  
2015 ◽  
Vol 74 ◽  
pp. 134-139 ◽  
Author(s):  
Hiroshige Sano ◽  
Junichi Kikuta ◽  
Masayuki Furuya ◽  
Naoki Kondo ◽  
Naoto Endo ◽  
...  
Keyword(s):  
Bone Imaging ◽  
Two Photon ◽  
Photon Excitation ◽  
Osteocytic Osteolysis ◽  
Two Photon Excitation

In vivo label-free two-photon excitation autofluorescence microscopy of microvasculature using a 520 nm femtosecond fiber laser

2020 ◽  
Vol 45 (10) ◽  
pp. 2704
Author(s):  
Ting Wu ◽  
Jiuling Liao ◽  
Jia Yu ◽  
Yufeng Gao ◽  
Hui Li ◽  
...  
Keyword(s):  
Fiber Laser ◽  
Label Free ◽  
Two Photon ◽  
Photon Excitation ◽  
Femtosecond Fiber Laser ◽  
Two Photon Excitation

scholarly journals A novel method for observing proteins in vivo using a small fluorescent label and multiphoton imaging

2005 ◽  
Vol 390 (3) ◽  
pp. 787-790 ◽  
Author(s):  
Stanley W. Botchway ◽  
Ignasi Barba ◽  
Randolf Jordan ◽  
Rebecca Harmston ◽  
Peter M. Haggie ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Fluorescence Emission ◽  
Yeast Cells ◽  
Fluorescent Label ◽  
Multiphoton Imaging ◽  
Two Photon ◽  
Photon Excitation ◽  
Novel Method ◽  
Two Photon Excitation

A novel method for the fluorescence detection of proteins in cells is described in the present study. Proteins are labelled by the selective biosynthetic incorporation of 5-hydroxytryptophan and the label is detected via selective two-photon excitation of the hydroxyindole and detection of its fluorescence emission at 340 nm. The method is demonstrated in this paper with images of a labelled protein in yeast cells.

scholarly journals In vivo live-cell imaging in plant tissues by two-photon excitation microscopy

2014 ◽  
Vol 26 (1) ◽  
pp. 25-30
Author(s):  
Yoko Mizuta ◽  
Daisuke Kurihara ◽  
Tetsuya Higashiyama
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Plant Tissues ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

scholarly journals Arduino Due based tool to facilitate in vivo two-photon excitation microscopy

2016 ◽  
Vol 7 (4) ◽  
pp. 1604 ◽  
Author(s):  
Pietro Artoni ◽  
Silvia Landi ◽  
Sebastian Sulis Sato ◽  
Stefano Luin ◽  
Gian Michele Ratto
Keyword(s):  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

scholarly journals Two-Photon Excitation of Azobenzene Photoswitches for Synthetic Optogenetics

2020 ◽  
Vol 10 (3) ◽  
pp. 805 ◽  
Author(s):  
Shai Kellner ◽  
Shai Berlin
Keyword(s):  
Visible Light ◽  
Light Illumination ◽  
Optical Technique ◽  
Tissue Penetration ◽  
Cellular Targets ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Synthetic optogenetics is an emerging optical technique that enables users to photocontrol molecules, proteins, and cells in vitro and in vivo. This is achieved by use of synthetic chromophores—denoted photoswitches—that undergo light-dependent changes (e.g., isomerization), which are meticulously designed to interact with unique cellular targets, notably proteins. Following light illumination, the changes adopted by photoswitches are harnessed to affect the function of nearby proteins. In most instances, photoswitches absorb visible light, wavelengths of poor tissue penetration, and excessive scatter. These shortcomings impede their use in vivo. To overcome these challenges, photoswitches of red-shifted absorbance have been developed. Notably, this shift in absorbance also increases their compatibility with two-photon excitation (2PE) methods. Here, we provide an overview of recent efforts devoted towards optimizing azobenzene-based photoswitches for 2PE and their current applications.

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