scholarly journals A novel method for observing proteins in vivo using a small fluorescent label and multiphoton imaging

2005 ◽  
Vol 390 (3) ◽  
pp. 787-790 ◽  
Author(s):  
Stanley W. Botchway ◽  
Ignasi Barba ◽  
Randolf Jordan ◽  
Rebecca Harmston ◽  
Peter M. Haggie ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Fluorescence Emission ◽  
Yeast Cells ◽  
Fluorescent Label ◽  
Multiphoton Imaging ◽  
Two Photon ◽  
Photon Excitation ◽  
Novel Method ◽  
Two Photon Excitation

A novel method for the fluorescence detection of proteins in cells is described in the present study. Proteins are labelled by the selective biosynthetic incorporation of 5-hydroxytryptophan and the label is detected via selective two-photon excitation of the hydroxyindole and detection of its fluorescence emission at 340 nm. The method is demonstrated in this paper with images of a labelled protein in yeast cells.

Side illumination fluorescence emission characteristics from a dye doped polymer optical fiber under two-photon excitation

2008 ◽  
Vol 47 (11) ◽  
pp. 1913 ◽  
Author(s):  
M. Sheeba ◽  
M. Rajesh ◽  
S. Mathew ◽  
V. P. N. Nampoori ◽  
C. P. G. Vallabhan ◽  
...  
Keyword(s):  
Optical Fiber ◽  
Fluorescence Emission ◽  
Emission Characteristics ◽  
Polymer Optical Fiber ◽  
Two Photon ◽  
Photon Excitation ◽  
Doped Polymer ◽  
Side Illumination ◽  
Two Photon Excitation

In vivo label-free two-photon excitation autofluorescence microscopy of microvasculature using a 520 nm femtosecond fiber laser

2020 ◽  
Vol 45 (10) ◽  
pp. 2704
Author(s):  
Ting Wu ◽  
Jiuling Liao ◽  
Jia Yu ◽  
Yufeng Gao ◽  
Hui Li ◽  
...  
Keyword(s):  
Fiber Laser ◽  
Label Free ◽  
Two Photon ◽  
Photon Excitation ◽  
Femtosecond Fiber Laser ◽  
Two Photon Excitation

Intravital bone imaging by two-photon excitation microscopy to identify osteocytic osteolysis in vivo

Bone ◽  
2015 ◽  
Vol 74 ◽  
pp. 134-139 ◽  
Author(s):  
Hiroshige Sano ◽  
Junichi Kikuta ◽  
Masayuki Furuya ◽  
Naoki Kondo ◽  
Naoto Endo ◽  
...  
Keyword(s):  
Bone Imaging ◽  
Two Photon ◽  
Photon Excitation ◽  
Osteocytic Osteolysis ◽  
Two Photon Excitation

Fluorescence detection of protoporphyrin IX in living cells: a comparative study on single- and two-photon excitation

2008 ◽  
Vol 13 (2) ◽  
pp. 024014 ◽  
Author(s):  
Sijia Lu ◽  
Ji-Yao Chen ◽  
Yu Zhang ◽  
Jiong Ma ◽  
Pei-Nan Wang ◽  
...  
Keyword(s):  
Comparative Study ◽  
Fluorescence Detection ◽  
Protoporphyrin Ix ◽  
Living Cells ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

scholarly journals In vivo multiphoton fluorescence imaging with polymer dots

2017 ◽  
Author(s):  
Ahmed M. Hassan ◽  
Xu Wu ◽  
Jeremy W. Jarrett ◽  
Shihan Xu ◽  
David R. Miller ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Multiphoton Imaging ◽  
Two Photon ◽  
Photon Excitation ◽  
Deep Imaging ◽  
Polymer Dots ◽  
Versatile Tool ◽  
Cortical Imaging ◽  
Semiconducting Polymer Dots

AbstractDeep in vivo imaging of vasculature requires small, bright, and photostable fluorophores suitable for multiphoton microscopy (MPM). Although semiconducting polymer dots (pdots) are an emerging class of highly fluorescent contrast agents with favorable advantages for the next generation of in vivo imaging, their use for deep multiphoton imaging has never before been demonstrated. Here we characterize the multiphoton properties of three pdot variants (CNPPV, PFBT, and PFPV) and demonstrate deep imaging of cortical microvasculature in C57 mice. Specifically, we measure the two-versus three-photon power dependence of these pdots and observe a clear three-photon excitation signature at wavelengths longer than 1300 nm, and a transition from two-photon to three-photon excitation within a 1060 – 1300 nm excitation range. Furthermore, we show that pdots enable in vivo two-photon imaging of cerebrovascular architecture in mice up to 850 μm beneath the pial surface using 800 nm excitation. In contrast with traditional multiphoton probes, we also demonstrate that the broad multiphoton absorption spectrum of pdots permits imaging at longer wavelengths (λex = 1,060 and 1225 nm). These wavelengths approach an ideal biological imaging wavelength near 1,300 nm and confer compatibility with a high-power ytterbium-fiber laser and a high pulse energy optical parametric amplifier, resulting in substantial improvements in signal-to-background ratio (>3.5-fold) and greater cortical imaging depths of 900 μm and 1300 μm. Ultimately, pdots are a versatile tool for MPM due to their extraordinary brightness and broad absorption, which will undoubtedly unlock the ability to interrogate deep structures in vivo.

scholarly journals In vivo live-cell imaging in plant tissues by two-photon excitation microscopy

2014 ◽  
Vol 26 (1) ◽  
pp. 25-30
Author(s):  
Yoko Mizuta ◽  
Daisuke Kurihara ◽  
Tetsuya Higashiyama
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Plant Tissues ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation
Sign in / Sign up

Export Citation Format

Share Document