scholarly journals Lipid-modified oligonucleotide conjugates: Insights into gene silencing, interaction with model membranes and cellular uptake mechanisms

2017 ◽  
Vol 25 (1) ◽  
pp. 175-186 ◽  
Author(s):  
Begoña Ugarte-Uribe ◽  
Santiago Grijalvo ◽  
Samuel Núñez Pertíñez ◽  
Jon V. Busto ◽  
César Martín ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Model Membranes ◽  
Oligonucleotide Conjugates ◽  
Modified Oligonucleotide ◽  
Uptake Mechanisms

scholarly journals Novel Lipid-Oligonucleotide Conjugates Containing Long-Chain Sulfonyl Phosphoramidate Groups: Synthesis and Biological Properties

2021 ◽  
Vol 11 (3) ◽  
pp. 1174
Author(s):  
Alina Derzhalova ◽  
Oleg Markov ◽  
Alesya Fokina ◽  
Yasuo Shiohama ◽  
Timofei Zatsepin ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Biological Properties ◽  
Dna And Rna ◽  
Guidance Molecules ◽  
Low Cytotoxicity ◽  
Oligonucleotide Conjugates ◽  
Long Chain

New lipid conjugates of DNA and RNA incorporating one to four [(4-dodecylphenyl)sulfonyl]phosphoramidate or (hexadecylsulfonyl)phosphoramidate groups at internucleotidic positions near the 3′ or 5′-end were synthesized and characterized. Low cytotoxicity of the conjugates and their ability to be taken up into cells without transfection agents were demonstrated. Lipid-conjugated siRNAs targeting repulsive guidance molecules a (RGMa) have shown a comparable gene silencing activity in PK-59 cells to unmodified control siRNA when delivered into the cells via Lipofectamine mediated transfection.

scholarly journals In vitro dosimetry of agglomerates

Nanoscale ◽  
2014 ◽  
Vol 6 (13) ◽  
pp. 7325-7331 ◽  
Author(s):  
V. Hirsch ◽  
C. Kinnear ◽  
L. Rodriguez-Lorenzo ◽  
C. A. Monnier ◽  
B. Rothen-Rutishauser ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Diffusion Coefficients ◽  
Uptake Kinetics ◽  
Small Particles ◽  
Au Nps ◽  
Kinetics Of ◽  
Lower Diffusion ◽  
Uptake Mechanisms ◽  
In Vitro Dosimetry

A well-controlled route towards biocompatible agglomerated Au-NPs is reported, which span the range from small particles with high diffusion coefficients to larger particles with lower diffusion coefficients. Difference in uptake kinetics of single NPs and agglomerates can be explained by particokinetics, without the need to consider size-mediated cellular uptake mechanisms.

scholarly journals siRNA Inhibition of Endocytic Pathways to Characterize the Cellular Uptake Mechanisms of Folate-Functionalized Glycol Chitosan Nanogels

2015 ◽  
Vol 12 (6) ◽  
pp. 1970-1979 ◽  
Author(s):  
Paula Pereira ◽  
Sílvia S. Pedrosa ◽  
Jennifer M. Wymant ◽  
Edward Sayers ◽  
Alexandra Correia ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Glycol Chitosan ◽  
Uptake Mechanisms ◽  
Endocytic Pathways

Enhanced cellular uptake and gene silencing activity of siRNA molecules mediated by chitosan-derivative nanocomplexes

2014 ◽  
Vol 473 (1-2) ◽  
pp. 579-590 ◽  
Author(s):  
Diana Guzman-Villanueva ◽  
Ibrahim M. El-Sherbiny ◽  
Alexander V. Vlassov ◽  
Dea Herrera-Ruiz ◽  
Hugh D.C. Smyth
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Chitosan Derivative

Cellular Uptake Mechanisms of Novel Anionic siRNA Lipoplexes

2012 ◽  
Vol 30 (4) ◽  
pp. 1161-1175 ◽  
Author(s):  
Mamta Kapoor ◽  
Diane J. Burgess
Keyword(s):  
Cellular Uptake ◽  
Uptake Mechanisms

scholarly journals Insights in cellular uptake mechanisms of pDNA–polycationic amphiphilic cyclodextrin nanoparticles (CDplexes)

2010 ◽  
Vol 143 (3) ◽  
pp. 318-325 ◽  
Author(s):  
Alejandro Díaz-Moscoso ◽  
Dries Vercauteren ◽  
Joanna Rejman ◽  
Juan M. Benito ◽  
Carmen Ortiz Mellet ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Uptake Mechanisms ◽  
Amphiphilic Cyclodextrin

Quaternized starch-based carrier for siRNA delivery: From cellular uptake to gene silencing

2014 ◽  
Vol 185 ◽  
pp. 109-120 ◽  
Author(s):  
Eliz Amar-Lewis ◽  
Aharon Azagury ◽  
Ramesh Chintakunta ◽  
Riki Goldbart ◽  
Tamar Traitel ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
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