Dual-Polymer-Functionalized Nanoscale Graphene Oxide as a Highly Effective Gene Transfection Agent for Insect Cells with Cell-Type-Dependent Cellular Uptake Mechanisms

2013 ◽  
Vol 30 (9) ◽  
pp. 794-803 ◽  
Author(s):  
Jing Zhang ◽  
Liangzhu Feng ◽  
Xiaofang Tan ◽  
Xiaozhe Shi ◽  
Ligeng Xu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Cellular Uptake ◽  
Insect Cells ◽  
Gene Transfection ◽  
Cell Type ◽  
Highly Effective ◽  
Transfection Agent ◽  
Uptake Mechanisms

scholarly journals Highly Effective Gene Transfection In Vivo by Alkylated Polyethylenimine

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Jennifer A. Fortune ◽  
Tatiana I. Novobrantseva ◽  
Alexander M. Klibanov
Keyword(s):  
Gene Expression ◽  
Gene Delivery ◽  
Cellular Uptake ◽  
Alkyl Chain ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Mouse Lung ◽  
Highly Effective ◽  
Chain Lengths

We mechanistically explored the effect of increased hydrophobicity of the polycation on the efficacy and specificity of gene delivery in mice. N-Alkylated linear PEIs with varying alkyl chain lengths and extent of substitution were synthesized and characterized by biophysical methods. Their in vivo transfection efficiency, specificity, and biodistribution were investigated. N-Ethylation improves the in vivo efficacy of gene expression in the mouse lung 26-fold relative to the parent polycation and more than quadruples the ratio of expression in the lung to that in all other organs. N-Propyl-PEI was the best performer in the liver and heart (581- and 3.5-fold enhancements, resp.) while N-octyl-PEI improved expression in the kidneys over the parent polymer 221-fold. As these enhancements in gene expression occur without changing the plasmid biodistribution, alkylation does not alter the cellular uptake but rather enhances transfection subsequent to cellular uptake.

Lateral size of graphene oxide determines differential cellular uptake and cell death pathways in Kupffer cells, LSECs, and hepatocytes

Nano Today ◽  
2021 ◽  
Vol 37 ◽  
pp. 101061
Author(s):  
Jiulong Li ◽  
Xiang Wang ◽  
Kuo-Ching Mei ◽  
Chong Hyun Chang ◽  
Jinhong Jiang ◽  
...  
Keyword(s):  
Cell Death ◽  
Graphene Oxide ◽  
Cellular Uptake ◽  
Kupffer Cells ◽  
Lateral Size ◽  
Cell Death Pathways

Printing ultrathin graphene oxide nanofiltration membranes for water purification

2017 ◽  
Vol 5 (39) ◽  
pp. 20860-20866 ◽  
Author(s):  
Mahdi Fathizadeh ◽  
Huynh Ngoc Tien ◽  
Konstantin Khivantsev ◽  
Jung-Tsai Chen ◽  
Miao Yu
Keyword(s):  
Graphene Oxide ◽  
Water Purification ◽  
Inkjet Printing ◽  
Low Cost ◽  
Nanofiltration Membranes ◽  
Highly Effective ◽  
First Time ◽  
Polymeric Supports ◽  
Effective Water

We demonstrated for the first time that inkjet printing can be a low-cost, easy, fast, and scalable method for depositing ultrathin (7.5–60 nm) uniform graphene oxide (GO) nanofiltration membranes on polymeric supports for highly effective water purification.

Label-free tracking of nanosized graphene oxide cellular uptake by confocal Raman microscopy

The Analyst ◽  
2018 ◽  
Vol 143 (15) ◽  
pp. 3686-3692 ◽  
Author(s):  
Marianna Eliášová Sohová ◽  
Michal Bodík ◽  
Peter Siffalovic ◽  
Nikola Bugárová ◽  
Martina Labudová ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Cellular Uptake ◽  
Cancer Diagnostics ◽  
Raman Microscopy ◽  
Two Dimensional ◽  
Confocal Raman Microscopy ◽  
Label Free ◽  
Confocal Raman ◽  
Diagnostics And Therapy

Graphene oxide (GO), a partially oxidized two-dimensional allotrope of carbon, is an attractive nanocarrier for cancer diagnostics and therapy.

scholarly journals In vitro dosimetry of agglomerates

Nanoscale ◽  
2014 ◽  
Vol 6 (13) ◽  
pp. 7325-7331 ◽  
Author(s):  
V. Hirsch ◽  
C. Kinnear ◽  
L. Rodriguez-Lorenzo ◽  
C. A. Monnier ◽  
B. Rothen-Rutishauser ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Diffusion Coefficients ◽  
Uptake Kinetics ◽  
Small Particles ◽  
Au Nps ◽  
Kinetics Of ◽  
Lower Diffusion ◽  
Uptake Mechanisms ◽  
In Vitro Dosimetry

A well-controlled route towards biocompatible agglomerated Au-NPs is reported, which span the range from small particles with high diffusion coefficients to larger particles with lower diffusion coefficients. Difference in uptake kinetics of single NPs and agglomerates can be explained by particokinetics, without the need to consider size-mediated cellular uptake mechanisms.

Fabrication of a phosphorylated graphene oxide–chitosan composite for highly effective and selective capture of U(vi)

2017 ◽  
Vol 4 (9) ◽  
pp. 1876-1886 ◽  
Author(s):  
Yawen Cai ◽  
Chunfang Wu ◽  
Zhiyong Liu ◽  
Linjuan Zhang ◽  
Lanhua Chen ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Sorption Capacity ◽  
High Sorption Capacity ◽  
Highly Effective ◽  
High Sorption ◽  
Chitosan Composite

The synthesized GO–CS–P exhibits ultrafast removal kinetics, high sorption capacity and great selectivity towards U(vi).

scholarly journals siRNA Inhibition of Endocytic Pathways to Characterize the Cellular Uptake Mechanisms of Folate-Functionalized Glycol Chitosan Nanogels

2015 ◽  
Vol 12 (6) ◽  
pp. 1970-1979 ◽  
Author(s):  
Paula Pereira ◽  
Sílvia S. Pedrosa ◽  
Jennifer M. Wymant ◽  
Edward Sayers ◽  
Alexandra Correia ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Glycol Chitosan ◽  
Uptake Mechanisms ◽  
Endocytic Pathways

Effects of cationic concentration on controlling the interlayer spacings for highly effective ion rejection via graphene oxide membranes

2020 ◽  
Vol 56 (18) ◽  
pp. 2743-2746
Author(s):  
Shuai Wang ◽  
Shanshan Liang ◽  
Liang Chen ◽  
Liuhua Mu ◽  
Gang Xu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Ion Rejection ◽  
Highly Effective ◽  
Oxide Membranes ◽  
Graphene Oxide Membranes

The interlayer spacings of graphene oxide membranes can be finely controlled using different concentrations of cations.

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