scholarly journals Rapid on-chip apoptosis assay on human carcinoma cells based on annexin-V/quantum dot probes

2017 ◽  
Vol 94 ◽  
pp. 408-414 ◽  
Author(s):  
Helena Montón ◽  
Mariana Medina-Sánchez ◽  
Joan Antoni Soler ◽  
Andrzej Chałupniak ◽  
Carme Nogués ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Annexin V ◽  
Carcinoma Cells ◽  
Human Carcinoma ◽  
Apoptosis Assay ◽  
On Chip

scholarly journals Metallothionein 3 Is a Hypoxia-Upregulated Oncogene Enhancing Cell Invasion and Tumorigenesis in Human Bladder Carcinoma Cells

2019 ◽  
Vol 20 (4) ◽  
pp. 980 ◽  
Author(s):  
Ke-Hung Tsui ◽  
Chen-Pang Hou ◽  
Kang-Shuo Chang ◽  
Yu-Hsiang Lin ◽  
Tsui-Hsia Feng ◽  
...  
Keyword(s):  
Bladder Carcinoma ◽  
Annexin V ◽  
Carcinoma Cells ◽  
Gene Expressions ◽  
T24 Cells ◽  
Apoptosis Assay ◽  
Induced Apoptosis ◽  
Bladder Carcinoma Cells

Metallothioneins have been viewed as modulators in a number of biological regulations regarding cancerous development; however, the function of metallothionein 3 (MT3) in bladder cancer is unexplored. We determined the regulatory mechanisms and potential function of MT3 in bladder carcinoma cells. Real-Time Reverse Transcriptase-Polymerase Chain Reaction (RT-qPCR) assays revealed that TSGH-8301 cells expressed more MT3 levels than RT-4, HT1376, and T24 cells. Immunoblot and RT-qPCR assays showed that arsenic (AS2O3) treatments enhanced the gene expression of MT3. Hypoxia induced HIF-1α, HIF-2α, and MT3 expression; furthermore, HIF-2α-knockdown attenuated hypoxic activation on MT3 expression. Ectopic overexpression of MT3 increased cell proliferation, invasion, and tumorigenesis significantly in T24 and HT1376 cells in vitro and in vivo; however, MT3-knockdown in TSGH-8301 cells had the reverse effect. Moreover, knockdown of MT3 enhanced arsenic-induced apoptosis determined by the Annexin V-FITC apoptosis assay. MT3-overexpression downregulated the gene expressions of N-myc downstream regulated gene 1 (NDRG1), N-myc downstream regulated gene 2 (NDRG2), and the mammary serine protease inhibitor (MASPIN) in HT1376 and T24 cells, whereas MT3-knockdown in TSGH-8301 cells had the opposite effect. The experiments indicated that MT3 is an arsenic- and hypoxia-upregulated oncogene that promotes cell growth and invasion of bladder carcinoma cells via downregulation of NDRG1, NDRG2, and MASPIN expressions.

scholarly journals Cattleianal and Cattleianone: Two New Meroterpenoids from Psidium cattleianum Leaves and Their Selective Antiproliferative Action against Human Carcinoma Cells

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2891
Author(s):  
Engy A. Mahrous ◽  
Ahmed M. Al-Abd ◽  
Maha M. Salama ◽  
Magda M. Fathy ◽  
Fathy M. Soliman ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Annexin V ◽  
Carcinoma Cells ◽  
Cell Cycle Analysis ◽  
Breast Adenocarcinoma ◽  
Human Carcinoma ◽  
Psidium Cattleianum ◽  
Antiproliferative Action ◽  
Adenocarcinoma Cells ◽  
Ht 29

The Myrteacae family is known as a rich source of phloroglucinols, a group of secondary metabolites with notable biological activities. Leaves of Psidium cattleianum were extracted with chloroform: methanol 8:2 to target the isolation of phloroglucinol derivatives. Isolated compounds were characterized using different spectroscopic methods: nuclear magnetic resonance (NMR), ultra-violet (UV) and mass spectrometry (MS). Two new phloroglucinols were evaluated for cytotoxicity against a panel of six human cancer cell lines, namely colorectal adenocarcinoma cells (HT-29 and HCT-116); hepatocellular carcinoma cells (HepG-2); laryngeal carcinoma (Hep-2); breast adenocarcinoma cells (MCF7 and MDA-MB231), in addition to normal human melanocytes HFB-4. Additionally, cell cycle analysis and annexin-V/FITC-staining were used to gain insights into the mechanism of action of the isolated compounds. The new phloroglucinol meroterpenoids, designated cattleianal and cattleianone, showed selective antiproliferative action against HT-29 cells with IC50’s of 35.2 and 32.1 μM, respectively. Results obtained using cell cycle analysis and annexin-V/FITC-staining implicated both necrosis and apoptosis pathways in the selective cytotoxicity of cattleianal and cattleianone. Our findings suggest that both compounds are selective antiproliferative agents and support further mechanistic studies for phloroglucinol meroterpenoids as scaffolds for developing new selective chemotherapeutic agents.

Mitogenic response of human carcinoma cells to the liver-derived hormone FGF21

2016 ◽  
Vol 11 (03) ◽  
Author(s):  
L Berti ◽  
B Rädle ◽  
HU Häring ◽  
M Hrab((ebrevis)) de Angelis ◽  
H Staiger
Keyword(s):  
Carcinoma Cells ◽  
Mitogenic Response ◽  
Human Carcinoma

scholarly journals Cellular and Molecular Aspects of Anti-Melanoma Effect of Minocycline—A Study of Cytotoxicity and Apoptosis on Human Melanotic Melanoma Cells

2020 ◽  
Vol 21 (18) ◽  
pp. 6917
Author(s):  
Jakub Rok ◽  
Zuzanna Rzepka ◽  
Artur Beberok ◽  
Justyna Pawlik ◽  
Dorota Wrześniok
Keyword(s):  
Myeloid Leukemia ◽  
Standard Treatment ◽  
Annexin V ◽  
Medical Problem ◽  
Melanoma Cells ◽  
Carcinoma Cells ◽  
Anti Cancer ◽  
Acute Myeloid Leukemia Cells ◽  
Molecular Aspects ◽  
Therapy Result

Minocycline is a tetracycline compound with pleiotropic pharmacological properties. In addition to its antibacterial action, it shows many non-antimicrobial effects, including an anti-cancer activity. The anti-cancer action was confirmed in studies on ovarian carcinoma cells, hepatocellular carcinoma cells, glioma cells, or acute myeloid leukemia cells. Malignant melanoma remains a serious medical problem despite the extensive knowledge of the disease. The low effectiveness of the standard treatment, as well as the resistance to therapy, result in high mortality rates. This work aimed to investigate the potential and mechanisms of anti-melanoma action of minocycline. Human skin melanotic melanoma cell line COLO 829 was used in the study. The obtained results showed that minocycline decreased cell viability and inhibited the growth of melanoma cells, proportional to the drug concentration as well as to the time of incubation. The EC50 values were calculated to be 78.6 µM, 31.7 µM, and 13.9 µM for 24 h, 48 h, and 72 h, respectively. It was observed that treated cells had a disturbed cell cycle and significantly changed morphology. Moreover, minocycline caused a decrease in mitochondrial membrane potential and an increase in cells with a low level of reduced thiols. Finally, it was found that the anti-melanoma effect of minocycline was related to the induction of apoptosis. The drug activated caspases 8, 9, and 3/7 as well as increased the number of annexin V-positive cells. The presented results show that minocycline possesses anti-melanoma potential.

Modeling cell response to low doses of photon irradiation: Part 2—application to radiation-induced chromosomal aberrations in human carcinoma cells

2015 ◽  
Vol 55 (1) ◽  
pp. 31-40 ◽  
Author(s):  
Micaela Cunha ◽  
Etienne Testa ◽  
Olga V. Komova ◽  
Elena A. Nasonova ◽  
Larisa A. Mel’nikova ◽  
...  
Keyword(s):  
Chromosomal Aberrations ◽  
Cell Response ◽  
Carcinoma Cells ◽  
Low Doses ◽  
Human Carcinoma ◽  
Photon Irradiation ◽  
Radiation Induced

scholarly journals Evidence for nonvectorial, retrograde transferrin trafficking in the early endosomes of HEp2 cells.

1995 ◽  
Vol 128 (4) ◽  
pp. 549-561 ◽  
Author(s):  
R N Ghosh ◽  
F R Maxfield
Keyword(s):  
Steady State ◽  
Digital Image Analysis ◽  
Carcinoma Cells ◽  
Exit Rate ◽  
Human Carcinoma ◽  
First Order ◽  
First Order Kinetics ◽  
Early Endosomes ◽  
Retrograde Traffic ◽  
Endosomal System

We have previously characterized the trafficking of transferrin (Tf) through HEp2 human carcinoma cells (Ghosh, R. N., D. L. Gelman, and F. R. Maxfield, 1994. J. Cell Sci. 107:2177-2189). Early endosomes in these cells are comprised of both sorting endosomes and recycling compartments, which are distinct separate compartments. Endocytosed Tf initially appears in punctate sorting endosomes that also contain recently endocytosed LDL. After short loading pulses, Tf rapidly sorts from LDL with first-order kinetics (t1/2 approximately 2.5 min), and it enters the recycling compartment before leaving the cell (t1/2 approximately 7 min). Here, we report a second, slower rate for Tf to leave sorting endosomes after HEp2 cells were labeled to steady state with fluorescein Tf instead of the brief pulse used previously. We determined this rate using digital image analysis to measure the Tf content of sorting endosomes that also contained LDL. With an 11-min chase, the Tf in sorting endosomes was 24% of steady-state value. This was in excess of the amount expected (5% of steady state) from the rate of Tf exit after short filling pulses. The excess could not be accounted for by reinternalization of recycled cell surface Tf, implying that either some Tf was retained in sorting endosomes, or that Tf was delivered back to the sorting endosomes from the recycling compartment. The former is unlikely since nearly all sorting endosomes contain detectable Tf after an 11-min chase, even though more than one third of the sorting endosomes were formed during the chase time. Furthermore, while observing living cells by confocal microscopy, we saw vesicle movements that appeared to be fluorescent Tf returning from recycling compartments to sorting endosomes. The slow rate of exit after steady-state labeling was similar to the Tf exit rate from the cell, suggesting an equilibration of Tf throughout the early endosomal system by this retrograde pathway. This retrograde traffic may be important for delivering molecules from the recycling compartment, which is a long-lived organelle, to sorting endosomes, which are transient.

Isolation and characterization of angiogenin, an angiogenic protein from human carcinoma cells

Biochemistry ◽  
1985 ◽  
Vol 24 (20) ◽  
pp. 5480-5486 ◽  
Author(s):  
James W. Fett ◽  
Daniel J. Strydom ◽  
Roy R. Lobb ◽  
Edward M. Alderman ◽  
J. Lemuel Bethune ◽  
...  
Keyword(s):  
Carcinoma Cells ◽  
Human Carcinoma ◽  
Isolation And Characterization

134 Molecular mechanisms of radiation-induced cell proliferation in human carcinoma cells

1996 ◽  
Vol 36 (1) ◽  
pp. 225
Author(s):  
R.K. Schmidt-Ullrich ◽  
R. Mikkelsen ◽  
K. Valerie ◽  
D. Todd ◽  
B. Kavanagh ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Molecular Mechanisms ◽  
Carcinoma Cells ◽  
Human Carcinoma ◽  
Radiation Induced

scholarly journals Efficient on-chip integration of a colloidal quantum dot photonic crystal band-edge laser with a coplanar waveguide

2017 ◽  
Vol 25 (26) ◽  
pp. 32919 ◽  
Author(s):  
Hyunho Jung ◽  
Myungjae Lee ◽  
Changhyun Han ◽  
Yeonsang Park ◽  
Kyung-Sang Cho ◽  
...  
Keyword(s):  
Photonic Crystal ◽  
Quantum Dot ◽  
Coplanar Waveguide ◽  
Band Edge ◽  
Colloidal Quantum Dot ◽  
On Chip
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