Nitrogen-rich functional groups carbon nanoparticles based fluorescent pH sensor with broad-range responding for environmental and live cells applications

2016 ◽  
Vol 82 ◽  
pp. 233-239 ◽  
Author(s):  
Bingfang Shi ◽  
Yubin Su ◽  
Liangliang Zhang ◽  
Rongjun Liu ◽  
Mengjiao Huang ◽  
...  
Keyword(s):  
Functional Groups ◽  
Carbon Nanoparticles ◽  
Ph Sensor ◽  
Live Cells

scholarly journals A protein–dye hybrid system as a narrow range tunable intracellular pH sensor

2016 ◽  
Vol 7 (11) ◽  
pp. 6808-6814 ◽  
Author(s):  
Palapuravan Anees ◽  
Karivachery V. Sudheesh ◽  
Purushothaman Jayamurthy ◽  
Arunkumar R. Chandrika ◽  
Ramakrishnapillai V. Omkumar ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Hybrid System ◽  
Narrow Range ◽  
Ph Sensor ◽  
Organic Dye ◽  
Live Cells ◽  
Two Component ◽  
Ph Variations

A two-component hybrid system consisting of organic dye nanoparticles (ash) and a protein (blue) at different ratios allows for the design of narrow range tunable nanoprobes for sensing and imaging of pH variations in live cells through ratiometric signalling.

Carbon dots of different composition and surface functionalization: cytotoxicity issues relevant to fluorescence cell imaging

2011 ◽  
Vol 236 (11) ◽  
pp. 1231-1238 ◽  
Author(s):  
Yanli Wang ◽  
Parambath Anilkumar ◽  
Li Cao ◽  
Jia-Hui Liu ◽  
Pengju G Luo ◽  
...  
Keyword(s):  
Surface Functionalization ◽  
Carbon Dots ◽  
Carbon Nanoparticles ◽  
Cell Imaging ◽  
Surface Passivation ◽  
Particle Surface ◽  
Live Cells ◽  
Carbon Particles

Nanoscale carbon particles have emerged as versatile precursors for a new class of highly fluorescent nanomaterials that resemble semiconductor quantum dots. The surface-passivated fluorescent carbon nanoparticles, dubbed ‘carbon dots’, were already demonstrated for their potential optical bioimaging applications in vitro and in vivo. In this study, we conducted a systematic cytotoxicity evaluation on the carbon dots prepared by various combinations of precursor carbon nanoparticles and molecules for the particle surface functionalization. The results suggested that the cytotoxicity of carbon dots was dependent on the selection of surface passivation molecules. Those dots showing more significant cytotoxicity at higher concentrations were also evaluated for their effects on the fluorescence imaging of live cells. The implications of the results on the eventual use of carbon dots as cell imaging agents are discussed.

scholarly journals Delivery of SiC-based nanoparticles into live cells driven by cell-penetrating peptides SAP and SAP-E

RSC Advances ◽  
2015 ◽  
Vol 5 (26) ◽  
pp. 20498-20502 ◽  
Author(s):  
T. Serdiuk ◽  
I. Bakanovich ◽  
V. Lysenko ◽  
S. A. Alekseev ◽  
V. A. Skryshevsky ◽  
...  
Keyword(s):  
Functional Groups ◽  
Cell Penetrating Peptides ◽  
Live Cells ◽  
Nanoparticle Delivery ◽  
Cell Penetrating ◽  
Covalent Conjugates

Cell-penetrating peptides enhance nanoparticle delivery into cells most efficiently if surface and peptide functional groups “match” to form non-covalent conjugates.

Fluorescent macrocyclic probes with pendant functional groups as markers of acidic organelles within live cells

2014 ◽  
Vol 12 (5) ◽  
pp. 823-831 ◽  
Author(s):  
Prashant D. Wadhavane ◽  
M. Ángeles Izquierdo ◽  
Dennis Lutters ◽  
M. Isabel Burguete ◽  
María J. Marín ◽  
...  
Keyword(s):  
Functional Groups ◽  
Live Cells ◽  
Acidic Organelles

A molecular beacon-based DNA switch for reversible pH sensing in vesicles and live cells

2016 ◽  
Vol 52 (56) ◽  
pp. 8741-8744 ◽  
Author(s):  
Nagarjun Narayanaswamy ◽  
Raji R. Nair ◽  
Y. V. Suseela ◽  
Deepak Kumar Saini ◽  
T. Govindaraju
Keyword(s):  
Structural Transformation ◽  
Molecular Beacon ◽  
Ph Sensor ◽  
Live Cells ◽  
Ph Sensing ◽  
Closed State

The DNA switch based on a molecular beacon (closed state) to A-motif (open state) structural transformation is developed as an efficient and reversible pH sensor in synthetic vesicles and live cells.

scholarly journals Litmus-Body: a Molecularly Targeted Sensor for Cell-Surface pH Measurements

2019 ◽  
Author(s):  
Marc C. Goudge ◽  
Joe Chin-Hun Kuo ◽  
Ann E. Metzloff ◽  
Ling-Ting Huang ◽  
Marshall J. Colville ◽  
...  
Keyword(s):  
Cell Surface ◽  
Fluorescent Protein ◽  
Stokes Shift ◽  
Ph Sensor ◽  
Endocytic Pathway ◽  
Live Cells ◽  
Ph Measurements ◽  
Ph Changes ◽  
Specific Proteins ◽  
Green Fluorescent

ABSTRACTPrecise pH measurements in the immediate environment of receptors is essential for elucidating the mechanisms through which local pH changes associated with diseased phenotypes manifest into aberrant receptor function. However, current pH sensors lack the molecular specificity required to make these measurements. Herein we present the Litmus-body, our recombinant protein-based pH sensor, which through fusion to an anti-mouse IgG nanobody is capable of molecular targeting to specific proteins on the cell surface. By normalizing a pH-dependent green fluorescent protein to a long-Stokes shift red fluorophore or fluorescent protein, we readily report pH independent of sensor concentration using a single 488-nm excitation. Our Litmus-body showed excellent responsiveness in solution, with a greater than 50-fold change across the physiological regime of pH. The sensor was further validated for use on live cells, shown to be specific to the protein of interest, and was able to successfully recapitulate the numerous pH changes along the endocytic pathway.

Fluorescent pH nanosensor based on carbon nanodots for monitoring minor intracellular pH changes

RSC Advances ◽  
2016 ◽  
Vol 6 (106) ◽  
pp. 104657-104664 ◽  
Author(s):  
Afsaneh Safavi ◽  
Raheleh Ahmadi ◽  
Zahra Mohammadpour ◽  
Jie Zhou
Keyword(s):  
Intracellular Ph ◽  
Ph Sensor ◽  
Live Cells ◽  
Carbon Nanodots ◽  
Ph Changes ◽  
Fluorescent Carbon Nanodots ◽  
Fluorescent Carbon

Fluorescent carbon nanodots were used as a sensitive, biocompatible intracellular pH sensor that can resolve minor pH differences in live cells.

scholarly journals Soybean-derived blue photoluminescent carbon dots

2020 ◽  
Vol 11 ◽  
pp. 606-619 ◽  
Author(s):  
Shanshan Wang ◽  
Wei Sun ◽  
Dong-sheng Yang ◽  
Fuqian Yang
Keyword(s):  
Functional Groups ◽  
Carbon Dots ◽  
Carbon Nanoparticles ◽  
Blue Emission ◽  
Hydrothermal Carbonization ◽  
Amorphous Structure ◽  
Surface Functional Groups ◽  
Carbon Particles ◽  
Ground Soybean ◽  
Green Strategy

Biomass-derived carbon dots (CDs) are biocompatible and have potential for a variety of applications, including bioimaging and biosensing. In this work, we use ground soybean residuals to synthesize carbon nanoparticles by hydrothermal carbonization (HTC), annealing at high temperature, and laser ablation (LA) in a NH4OH solution. The carbon nanoparticles synthesized with the HTC process (HTC-CDs) exhibit photoluminescent characteristics with strong blue emission. The annealing of the HTC-processed carbon particles in the range of 250 to 850 °C causes a loss of the photoluminescent characteristics of the CDs without any significant change in the microstructure (amorphous structure) of the carbon particles. The LA processing of the annealed HTC-processed carbon particles introduces nitrogen-containing surface-functional groups and leads to the recovery of the photoluminescent features that are different from those of the HTC-CDs and dependent on the fraction of nitrogen in the surface-functional groups. The photoluminescence of both the HTC-CDs and LA-CDs is largely due to the presence of N-containing surface-functional groups. The quantum yield of the LA-CDs is more constant than that of the HTC-CDs under continuous UV excitation and does not exhibit a significant reduction after 150 min of excitation. The methods used in this work provide a simple and green strategy to introduce N-surface-functional groups to carbon nanoparticles made from biomass and biowaste and to produce stable photoluminescent CDs with excellent water-wettability.

scholarly journals FLIM-Based Intracellular and Extracellular pH Measurements Using Genetically Encoded pH Sensor

Biosensors ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 340
Author(s):  
Alexander S. Goryashchenko ◽  
Alexey A. Pakhomov ◽  
Anastasia V. Ryabova ◽  
Igor D. Romanishkin ◽  
Eugene G. Maksimov ◽  
...  
Keyword(s):  
Cell Biology ◽  
Cytoplasmic Membrane ◽  
Transmembrane Domain ◽  
Fluorescent Sensor ◽  
Ph Sensor ◽  
Extracellular Ph ◽  
Live Cells ◽  
Ph Measurements ◽  
Quantitative Measurements

The determination of pH in live cells and tissues is of high importance in physiology and cell biology. In this report, we outline the process of the creation of SypHerExtra, a genetically encoded fluorescent sensor that is capable of measuring extracellular media pH in a mildly alkaline range. SypHerExtra is a protein created by fusing the previously described pH sensor SypHer3s with the neurexin transmembrane domain that targets its expression to the cytoplasmic membrane. We showed that with excitation at 445 nm, the fluorescence lifetime of both SypHer3s and SypHerExtra strongly depend on pH. Using FLIM microscopy in live eukaryotic cells, we demonstrated that SypHerExtra can be successfully used to determine extracellular pH, while SypHer3s can be applied to measure intracellular pH. Thus, these two sensors are suitable for quantitative measurements using the FLIM method, to determine intracellular and extracellular pH in a range from pH 7.5 to 9.5 in different biological systems.

scholarly journals Soybean-derived blue photoluminescent carbon dots

2019 ◽  
Author(s):  
Shanshan Wang ◽  
Wei Sun ◽  
Dong-sheng Yang ◽  
Fuqian Yang
Keyword(s):  
Functional Groups ◽  
Carbon Dots ◽  
Carbon Nanoparticles ◽  
Blue Emission ◽  
Hydrothermal Carbonization ◽  
Amorphous Structure ◽  
Surface Functional Groups ◽  
Carbon Particles ◽  
Bio Imaging ◽  
Ground Soybean

Biomass-derived carbon dots (CDs) are biocompatible and have the potential in a variety of applications, including bio-imaging and bio-sensing. In this work, we use ground soybean residuals to synthesize carbon nanoparticles by hydrothermal carbonization (HTC), annealing at high temperatures, and laser ablation (LA) in a NH4OH solution. The carbon nanoparticles synthesized with the HTC process (HTC-CDs) exhibit photoluminescent characteristics with strong blue emission. The annealing of the HTC-CDs at temperatures in a range of 250 to 850 ⁰C causes the loss of the photoluminescent characteristics without any significant changes in the microstructures (amorphous structure) of carbon particles. The LA processing of the annealed HTC-CDs introduces nitrogen-contained surface-functional groups and leads to the recovery of the photoluminescent features that are different from those of the HTC-CDs and dependent on the fraction of nitrogen in the surface-functional groups. The photoluminescence of both the HTC-CDs and LA-CDs is largely due to the presence of N-contained surface-functional groups. The quantum yield of the LA-CDs is more stable than that of the HTC-CDs under continuous UV excitation and does not exhibit significant reduction over 150 min excitation. The methods used in this work provide a simple and green strategy to introduce surface-functional groups to carbon nanoparticles from biomass and biowaste and to produce stable photoluminescent CDs with excellent water-wettability.

Sign in / Sign up

Export Citation Format

Share Document