Fluorescent macrocyclic probes with pendant functional groups as markers of acidic organelles within live cells

2014 ◽  
Vol 12 (5) ◽  
pp. 823-831 ◽  
Author(s):  
Prashant D. Wadhavane ◽  
M. Ángeles Izquierdo ◽  
Dennis Lutters ◽  
M. Isabel Burguete ◽  
María J. Marín ◽  
...  
Keyword(s):  
Functional Groups ◽  
Live Cells ◽  
Acidic Organelles

Synthetic Macrocyclic Peptidomimetics as Tunable pH Probes for the Fluorescence Imaging of Acidic Organelles in Live Cells

2005 ◽  
Vol 117 (40) ◽  
pp. 6662-6666 ◽  
Author(s):  
Francisco Galindo ◽  
M. Isabel Burguete ◽  
Laura Vigara ◽  
Santiago V. Luis ◽  
Nurul Kabir ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Live Cells ◽  
Acidic Organelles ◽  
Ph Probes

Nitrogen-rich functional groups carbon nanoparticles based fluorescent pH sensor with broad-range responding for environmental and live cells applications

2016 ◽  
Vol 82 ◽  
pp. 233-239 ◽  
Author(s):  
Bingfang Shi ◽  
Yubin Su ◽  
Liangliang Zhang ◽  
Rongjun Liu ◽  
Mengjiao Huang ◽  
...  
Keyword(s):  
Functional Groups ◽  
Carbon Nanoparticles ◽  
Ph Sensor ◽  
Live Cells

scholarly journals A novel acidotropic pH indicator and its potential application in labeling acidic organelles of live cells

1999 ◽  
Vol 6 (7) ◽  
pp. 411-418 ◽  
Author(s):  
Zhenjun Diwu ◽  
Chii-Shiarng Chen ◽  
Cailan Zhang ◽  
Dieter H Klaubert ◽  
Richard P Haugland
Keyword(s):  
Potential Application ◽  
Live Cells ◽  
Ph Indicator ◽  
Acidic Organelles

A novel fluorescent probe for selective detection of thiols in acidic solutions and labeling of acidic organelles in live cells

2013 ◽  
Vol 1 (4) ◽  
pp. 438-442 ◽  
Author(s):  
Qin-Hua Song ◽  
Qing-Qing Wu ◽  
Chang-Hui Liu ◽  
Xiao-Jiao Du ◽  
Qing-Xiang Guo
Keyword(s):  
Fluorescent Probe ◽  
Live Cells ◽  
Selective Detection ◽  
Acidic Solutions ◽  
Acidic Organelles

Synthetic Macrocyclic Peptidomimetics as Tunable pH Probes for the Fluorescence Imaging of Acidic Organelles in Live Cells

2005 ◽  
Vol 44 (40) ◽  
pp. 6504-6508 ◽  
Author(s):  
Francisco Galindo ◽  
M. Isabel Burguete ◽  
Laura Vigara ◽  
Santiago V. Luis ◽  
Nurul Kabir ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Live Cells ◽  
Acidic Organelles ◽  
Ph Probes

scholarly journals Delivery of SiC-based nanoparticles into live cells driven by cell-penetrating peptides SAP and SAP-E

RSC Advances ◽  
2015 ◽  
Vol 5 (26) ◽  
pp. 20498-20502 ◽  
Author(s):  
T. Serdiuk ◽  
I. Bakanovich ◽  
V. Lysenko ◽  
S. A. Alekseev ◽  
V. A. Skryshevsky ◽  
...  
Keyword(s):  
Functional Groups ◽  
Cell Penetrating Peptides ◽  
Live Cells ◽  
Nanoparticle Delivery ◽  
Cell Penetrating ◽  
Covalent Conjugates

Cell-penetrating peptides enhance nanoparticle delivery into cells most efficiently if surface and peptide functional groups “match” to form non-covalent conjugates.

Novel long-wavelength emissive lysosome-targeting ratiometric fluorescent probes for imaging in live cells

The Analyst ◽  
2019 ◽  
Vol 144 (14) ◽  
pp. 4288-4294 ◽  
Author(s):  
Jinyin Ge ◽  
Kai Zhang ◽  
Li Fan ◽  
Xiaodong Wang ◽  
Caihong Zhang ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Hydrolytic Enzymes ◽  
Live Cells ◽  
Long Wavelength ◽  
Acidic Organelles

Lysosomes are acidic organelles containing many hydrolytic enzymes responsible for degrading macromolecules.

scholarly journals Mahogunin Ring Finger 1 regulates pigmentation by controlling the pH of melanosomes in melanocytes and melanoma cells

2021 ◽  
Author(s):  
Julia Sirés-Campos ◽  
Ana Lambertos ◽  
Cédric Delevoye ◽  
Graça Raposo ◽  
Dorothy C. Bennett ◽  
...  
Keyword(s):  
Transient Receptor Potential ◽  
Specific Activity ◽  
Ring Finger ◽  
Receptor Potential ◽  
Transient Receptor Potential Channel ◽  
Melanoma Cells ◽  
Live Cells ◽  
Dependent Manner ◽  
Transient Receptor ◽  
Acidic Organelles

AbstractMahogunin Ring Finger 1 (MGRN1) is an E3-ubiquitin ligase absent in dark-furred mahoganoid mice. We investigated the mechanisms of hyperpigmentation in Mgrn1-null melan-md1 melanocytes, Mgrn1-KO cells obtained by CRISPR-Cas9-mediated knockdown of Mgrn1 in melan-a6 melanocytes, and melan-a6 cells depleted of MGRN1 by siRNA treatment. Mgrn1-deficient melanocytes showed higher melanin content associated with increased melanosome abundance and higher fraction of melanosomes in highly melanized maturation stages III–IV. Expression, post-translational processing and enzymatic activity of the rate-limiting melanogenic enzyme tyrosinase measured in cell-free extracts were comparable in control and MGRN1-depleted cells. However, tyrosinase activity measured in situ in live cells and expression of genes associated with regulation of pH increased upon MGRN1 repression. Using pH-sensitive fluorescent probes, we found that downregulation of MGRN1 expression in melanocytes and melanoma cells increased the pH of acidic organelles, including melanosomes, strongly suggesting a previously unknown role of MGRN1 in the regulation of melanosomal pH. Among the pH regulatory genes upregulated by Mgrn1 knockdown, we identified those encoding several subunits of the vacuolar adenosine triphosphatase V-ATPase (mostly Atp6v0d2) and a calcium channel of the transient receptor potential channel family, Mucolipin 3 (Mcoln3). Manipulation of expression of the Mcoln3 gene showed that overexpression of Mcoln3 played a significant role in neutralization of the pH of acidic organelles and activation of tyrosinase in MGRN1-depleted cells. Therefore, lack of MGRN1 led to cell-autonomous stimulation of pigment production in melanocytes mostly by increasing tyrosinase specific activity through neutralization of the melanosomal pH in a MCOLN3-dependent manner.

4-dimensional, high-resolution imaging of living cells

1992 ◽  
Vol 50 (1) ◽  
pp. 416-417
Author(s):  
Shinya Inoué
Keyword(s):  
Light Microscope ◽  
Living Cells ◽  
Live Cells ◽  
Video Tape ◽  
Active Living ◽  
High Resolution Imaging ◽  
3 Dimensional ◽  
Dynamic Architecture ◽  
Resolution Imaging ◽  
Disk Memory

This paper reports progress of our effort to rapidly capture, and display in time-lapsed mode, the 3-dimensional dynamic architecture of active living cells and developing embryos at the highest resolution of the light microscope. Our approach entails: (A) real-time video tape recording of through-focal, ultrathin optical sections of live cells at the highest resolution of the light microscope; (B) repeat of A at time-lapsed intervals; (C) once each time-lapsed interval, an image at home focus is recorded onto Optical Disk Memory Recorder (OMDR); (D) periods of interest are selected using the OMDR and video tape records; (E) selected stacks of optical sections are converted into plane projections representing different view angles (±4 degrees for stereo view, additional angles when revolving stereos are desired); (F) analysis using A - D.

Multi-mode light microscopy of microtubule assembly dynamics and chromosome movement in vivo and In vitro

1996 ◽  
Vol 54 ◽  
pp. 730-731
Author(s):  
E. D. Salmon ◽  
J. C. Waters ◽  
C. Waterman-Storer
Keyword(s):  
Imaging System ◽  
Ccd Camera ◽  
Transmitted Light ◽  
Microtubule Assembly ◽  
Live Cells ◽  
Optical Efficiency ◽  
Multiple Band ◽  
Multi Mode

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.

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