Immunodevice for simultaneous detection of two relevant tumor markers based on separation of different microparticles by dielectrophoresis

2011 ◽  
Vol 28 (1) ◽  
pp. 443-449 ◽  
Author(s):  
Javier Ramón-Azcón ◽  
Tomoyuki Yasukawa ◽  
Fumio Mizutani
Keyword(s):  
Tumor Markers ◽  
Simultaneous Detection

scholarly journals Magnetic Control of an Electrochemical Microfluidic Device with an Arrayed Immunosensor for Simultaneous Multiple Immunoassays

2007 ◽  
Vol 53 (7) ◽  
pp. 1323-1329 ◽  
Author(s):  
Dianping Tang ◽  
Ruo Yuan ◽  
Yaqin Chai
Keyword(s):  
Tumor Markers ◽  
Microfluidic Device ◽  
Simultaneous Detection ◽  
Reference Electrode ◽  
Cost Effective ◽  
Sio2 Nanoparticles ◽  
Magnetic Bead ◽  
Label Free ◽  
Specific Tumor ◽  
Protein Diagnostics

Abstract Background: Methods based on magnetic bead probes have been developed for immunoassay, but most involve complicated labeling or stripping procedures and are unsuitable for routine use. Methods: We synthesized magnet core/shell NiFe2O4/SiO2 nanoparticles and fabricated an electrochemical magnetic controlled microfluidic device for the detection of 4 tumor markers. The immunoassay system consisted of 5 working electrodes and an Ag/AgCl reference electrode integrated on a glass substrate. Each working electrode contained a different antibody immobilized on the NiFe2O4/SiO2 nanoparticle surface and was capable of measuring a specific tumor marker using noncompetitive electrochemical immunoassay. Results: Under optimal conditions, the multiplex immunoassay enabled the simultaneous detection of 4 tumor markers. The sensor detection limit was <0.5 μg/L (or <0.5 kunits/L) for most analytes. Intra- and interassay imprecisions (CVs) were <4.5% and 8.7% for analyte concentrations >5 μg/L (or >5 kunits/L), respectively. No nonspecific adsorption was observed during a series of procedures to detect target proteins, and electrochemical cross-talk (CV) between neighboring sites was <10%. Conclusion: This immunoassay system offers promise for label-free, rapid, simple, cost-effective analysis of biological samples. Importantly, the chip-based immunosensor could be suitable for use in the mass production of miniaturized lab-on-a-chip devices and open new opportunities for protein diagnostics and biosecurity.

Polydopamine nanosphere@silver nanoclusters for fluorescence detection of multiplex tumor markers

Nanoscale ◽  
2019 ◽  
Vol 11 (17) ◽  
pp. 8119-8123 ◽  
Author(s):  
Yiting Jiang ◽  
Yuguo Tang ◽  
Peng Miao
Keyword(s):  
Tumor Markers ◽  
Fluorescence Detection ◽  
Simultaneous Detection ◽  
Fluorescence Method ◽  
Silver Nanoclusters ◽  
Silver Nanocluster ◽  
System P

An innovative fluorescence method is developed for simultaneous detection of multiplex tumor markers based on a polydopamine nanosphere@silver nanocluster system.

scholarly journals A Disposable Multianalyte Electrochemical Immunosensor Array for Automated Simultaneous Determination of Tumor Markers

2007 ◽  
Vol 53 (8) ◽  
pp. 1495-1502 ◽  
Author(s):  
Jie Wu ◽  
Feng Yan ◽  
Jinhai Tang ◽  
Chun Zhai ◽  
Huangxian Ju
Keyword(s):  
Carcinoembryonic Antigen ◽  
Tumor Markers ◽  
High Throughput ◽  
Simultaneous Detection ◽  
Detection Limits ◽  
Injection Technique ◽  
Human Choriogonadotropin ◽  
Multianalyte Detection ◽  
Multianalyte Immunoassay ◽  
Screen Printed

Abstract Background: Automated and convenient multianalyte detection with high throughput is increasingly needed in clinical diagnosis. We developed a disposable 4-by-2 array for programmed simultaneous amperometric immunoassay of 4 tumor markers. Methods: We used a screen-printed technique, 1-step immobilization method, and flow injection technique. We immobilized carcinoembryonic antigen, α-fetoprotein, β-human choriogonadotropin, and carcinoma antigen 125 as model analytes in a redox mediator–grafted, biopolymer-modified, screen-printed carbon electrode array to capture corresponding horseradish peroxidase-labeled antibodies in competitive immunoreactions. The simultaneous multianalyte immunoassay was automatically carried out to amperometrically monitor the mediator-catalyzed enzymatic response to hydrogen peroxide, which decreased in proportion to the concentrations of analytes in samples. Results: The multianalyte immunosensor array had a throughput of 60 samples/h and allowed simultaneous detection of carcinoembryonic antigen, α-fetoprotein, β-human choriogonadotropin, and carcinoma antigen 125 in clinical serum samples with concentrations up to 188 μg/L, 250 μg/L, 266 IU/L, and 334 kIU/L, respectively. The detection limits (limits of the blank, mean of blank plus 3 SD) were 1.1 μg/L, 1.7 μg/L, 1.2 IU/L, and 1.7 kIU/L. The inter- and intraassay imprecision (CVs) of the immunosensor arrays were <7.8% and <9.0%, respectively. The immunosensor arrays were stable for 28 days. Conclusions: This newly constructed immunosensor array provides a simple, automated, simultaneous multianalyte immunoassay with high throughput, short analytical time, and sufficiently low detection limits for clinical application. This method offers the capability of miniaturizing the multianalyte detection device.

Dual-wavelength responsive photoelectrochemical aptasensor based on ionic liquid functionalized Zn-MOF and noble metal nanoparticles for the simultaneous detection of multiple tumor markers

Nanoscale ◽  
2021 ◽  
Author(s):  
Yingying Zhong ◽  
xian Wang ◽  
Ruyan Zha ◽  
Chen Wang ◽  
Huijuan Zhang ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Tumor Marker ◽  
Metal Nanoparticles ◽  
Tumor Markers ◽  
Noble Metal ◽  
Simultaneous Detection ◽  
Noble Metal Nanoparticles ◽  
Joint Detection ◽  
Multiple Tumor ◽  
Single Tumor

A single tumor marker may correspond to a variety of diseases, and a specific disease requires the joint detection of multiple tumor markers for improving the accuracy of diagnoses. An...

Simultaneous fluoroimmunoassay of two tumor markers based on CdTe quantum dots and gold nanocluster coated-silica nanospheres as labels

RSC Advances ◽  
2015 ◽  
Vol 5 (128) ◽  
pp. 105992-105998 ◽  
Author(s):  
Lihua Li ◽  
Dexiang Feng ◽  
Junqing Zhao ◽  
Zilin Guo ◽  
Yuzhong Zhang
Keyword(s):  
Quantum Dots ◽  
Tumor Markers ◽  
Simultaneous Detection ◽  
Cdte Quantum Dots ◽  
Silica Nanospheres ◽  
Gold Nanocluster

Fluoroimmunoassay for simultaneous detection of CEA and AFP.

Sensitive electrochemical immunosensor array for the simultaneous detection of multiple tumor markers

The Analyst ◽  
2012 ◽  
Vol 137 (2) ◽  
pp. 393-399 ◽  
Author(s):  
Honglan Qi ◽  
Chen Ling ◽  
Qingyun Ma ◽  
Qiang Gao ◽  
Chengxiao Zhang
Keyword(s):  
Tumor Markers ◽  
Simultaneous Detection ◽  
Electrochemical Immunosensor ◽  
Multiple Tumor

Rapid and simple immunosensing system for simultaneous detection of tumor markers based on negative-dielectrophoretic manipulation of microparticles

Talanta ◽  
2010 ◽  
Vol 81 (1-2) ◽  
pp. 657-663 ◽  
Author(s):  
Hyun Jung Lee ◽  
Sang Hyun Lee ◽  
Tomoyuki Yasukawa ◽  
Javier Ramón-Azcón ◽  
Fumio Mizutani ◽  
...  
Keyword(s):  
Tumor Markers ◽  
Simultaneous Detection
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