Sensitive electrochemical immunosensor array for the simultaneous detection of multiple tumor markers

The Analyst ◽  
2012 ◽  
Vol 137 (2) ◽  
pp. 393-399 ◽  
Author(s):  
Honglan Qi ◽  
Chen Ling ◽  
Qingyun Ma ◽  
Qiang Gao ◽  
Chengxiao Zhang
Keyword(s):  
Tumor Markers ◽  
Simultaneous Detection ◽  
Electrochemical Immunosensor ◽  
Multiple Tumor

Dual-wavelength responsive photoelectrochemical aptasensor based on ionic liquid functionalized Zn-MOF and noble metal nanoparticles for the simultaneous detection of multiple tumor markers

Nanoscale ◽  
2021 ◽  
Author(s):  
Yingying Zhong ◽  
xian Wang ◽  
Ruyan Zha ◽  
Chen Wang ◽  
Huijuan Zhang ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Tumor Marker ◽  
Metal Nanoparticles ◽  
Tumor Markers ◽  
Noble Metal ◽  
Simultaneous Detection ◽  
Noble Metal Nanoparticles ◽  
Joint Detection ◽  
Multiple Tumor ◽  
Single Tumor

A single tumor marker may correspond to a variety of diseases, and a specific disease requires the joint detection of multiple tumor markers for improving the accuracy of diagnoses. An...

Simultaneous Detection of Multiple Tumor Markers in Blood by Functional Liquid Crystal Sensors Assisted with Target-Induced Dissociation of Aptamer

2020 ◽  
Vol 92 (5) ◽  
pp. 3867-3873 ◽  
Author(s):  
Lubin Qi ◽  
Shuya Liu ◽  
Yifei Jiang ◽  
Jin-Ming Lin ◽  
Li Yu ◽  
...  
Keyword(s):  
Liquid Crystal ◽  
Tumor Markers ◽  
Simultaneous Detection ◽  
Multiple Tumor

Evaluation of a method for the simultaneous detection of multiple tumor markers using a multiplex suspension bead array

2012 ◽  
Vol 45 (16-17) ◽  
pp. 1394-1398 ◽  
Author(s):  
Yajie Wang ◽  
Fang Fang ◽  
Congning Shi ◽  
Xulong Zhang ◽  
Li Liu ◽  
...  
Keyword(s):  
Tumor Markers ◽  
Simultaneous Detection ◽  
Multiple Tumor ◽  
Suspension Bead Array

scholarly journals Disposable Reagentless Electrochemical Immunosensor Array Based on a Biopolymer/Sol-Gel Membrane for Simultaneous Measurement of Several Tumor Markers

2008 ◽  
Vol 54 (9) ◽  
pp. 1481-1488 ◽  
Author(s):  
Jie Wu ◽  
Feng Yan ◽  
Xiaoqing Zhang ◽  
Yuetian Yan ◽  
Jinhai Tang ◽  
...  
Keyword(s):  
Tumor Markers ◽  
Point Of Care ◽  
Sol Gel ◽  
Simultaneous Detection ◽  
Carbohydrate Antigen ◽  
Electrochemical Immunosensor ◽  
Point Of Care Testing ◽  
Serum Samples ◽  
Sampling Volume ◽  
Analytical Time

Abstract Background: A reagentless sensor array for simultaneous multianalyte testing (SMAT) may enable accurate diagnosis and be applicable for point-of-care testing. We developed a disposable reagentless immunosensor array for simple immunoassay of panels of tumor markers. Methods: We carried out SMAT with a direct capture format, in which colloidal gold nanoparticles with bound horseradish peroxidase (HRP)-labeled antibodies were immobilized on screen-printed carbon electrodes with biopolymer/sol-gel to trap their corresponding antigens from sample solution. Upon formation of immunocomplex, the direct electrochemical signal of the HRP decreased owing to increasing spatial blocking, and the analytes could be simultaneously determined by monitoring the signal changes. Results: The proposed reagentless immunosensor array allowed simultaneous detection of carcinoma antigen 153, carcinoma antigen 125, carbohydrate antigen 199, and carcinoembryonic antigen in clinical serum samples in the ranges of 0.4–140 kU/L, 0.5–330 kU/L, 0.8–190 kU/L, and 0.1–44 μg/L, respectively, with detection limits of 0.2 kU/L, 0.5 kU/L, 0.3 kU/L, and 0.1 μg/L corresponding to the signals 3 SD above the mean of a zero standard. The interassay imprecision of the arrays was <9.5%, and they were stable for 35 days. The positivity detection rate of panels of tumor markers was >95.5% for 95 cases of cancer-positive sera. Conclusions: The immunosensor array provides a SMAT with short analytical time, small sampling volume, no need for substrate, and, no between-electrode cross-talk. This method not only proved the capability of the array in point-of-care testing, but also allowed simultaneous testing of several tumor markers.

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