A novel dual-template molecularly imprinted electrochemiluminescence immunosensor array using Ru(bpy)32+-Silica@Poly-L-lysine-Au composite nanoparticles as labels for near-simultaneous detection of tumor markers

2014 ◽  
Vol 139 ◽  
pp. 127-136 ◽  
Author(s):  
Xiaobin Feng ◽  
Ning Gan ◽  
Jing Zhou ◽  
Tianhua Li ◽  
Yuting Cao ◽  
...  
Keyword(s):  
Tumor Markers ◽  
Simultaneous Detection ◽  
Composite Nanoparticles ◽  
Molecularly Imprinted

scholarly journals Magnetic Control of an Electrochemical Microfluidic Device with an Arrayed Immunosensor for Simultaneous Multiple Immunoassays

2007 ◽  
Vol 53 (7) ◽  
pp. 1323-1329 ◽  
Author(s):  
Dianping Tang ◽  
Ruo Yuan ◽  
Yaqin Chai
Keyword(s):  
Tumor Markers ◽  
Microfluidic Device ◽  
Simultaneous Detection ◽  
Reference Electrode ◽  
Cost Effective ◽  
Sio2 Nanoparticles ◽  
Magnetic Bead ◽  
Label Free ◽  
Specific Tumor ◽  
Protein Diagnostics

Abstract Background: Methods based on magnetic bead probes have been developed for immunoassay, but most involve complicated labeling or stripping procedures and are unsuitable for routine use. Methods: We synthesized magnet core/shell NiFe2O4/SiO2 nanoparticles and fabricated an electrochemical magnetic controlled microfluidic device for the detection of 4 tumor markers. The immunoassay system consisted of 5 working electrodes and an Ag/AgCl reference electrode integrated on a glass substrate. Each working electrode contained a different antibody immobilized on the NiFe2O4/SiO2 nanoparticle surface and was capable of measuring a specific tumor marker using noncompetitive electrochemical immunoassay. Results: Under optimal conditions, the multiplex immunoassay enabled the simultaneous detection of 4 tumor markers. The sensor detection limit was <0.5 μg/L (or <0.5 kunits/L) for most analytes. Intra- and interassay imprecisions (CVs) were <4.5% and 8.7% for analyte concentrations >5 μg/L (or >5 kunits/L), respectively. No nonspecific adsorption was observed during a series of procedures to detect target proteins, and electrochemical cross-talk (CV) between neighboring sites was <10%. Conclusion: This immunoassay system offers promise for label-free, rapid, simple, cost-effective analysis of biological samples. Importantly, the chip-based immunosensor could be suitable for use in the mass production of miniaturized lab-on-a-chip devices and open new opportunities for protein diagnostics and biosecurity.

Polydopamine nanosphere@silver nanoclusters for fluorescence detection of multiplex tumor markers

Nanoscale ◽  
2019 ◽  
Vol 11 (17) ◽  
pp. 8119-8123 ◽  
Author(s):  
Yiting Jiang ◽  
Yuguo Tang ◽  
Peng Miao
Keyword(s):  
Tumor Markers ◽  
Fluorescence Detection ◽  
Simultaneous Detection ◽  
Fluorescence Method ◽  
Silver Nanoclusters ◽  
Silver Nanocluster ◽  
System P

An innovative fluorescence method is developed for simultaneous detection of multiplex tumor markers based on a polydopamine nanosphere@silver nanocluster system.

scholarly journals A Disposable Multianalyte Electrochemical Immunosensor Array for Automated Simultaneous Determination of Tumor Markers

2007 ◽  
Vol 53 (8) ◽  
pp. 1495-1502 ◽  
Author(s):  
Jie Wu ◽  
Feng Yan ◽  
Jinhai Tang ◽  
Chun Zhai ◽  
Huangxian Ju
Keyword(s):  
Carcinoembryonic Antigen ◽  
Tumor Markers ◽  
High Throughput ◽  
Simultaneous Detection ◽  
Detection Limits ◽  
Injection Technique ◽  
Human Choriogonadotropin ◽  
Multianalyte Detection ◽  
Multianalyte Immunoassay ◽  
Screen Printed

Abstract Background: Automated and convenient multianalyte detection with high throughput is increasingly needed in clinical diagnosis. We developed a disposable 4-by-2 array for programmed simultaneous amperometric immunoassay of 4 tumor markers. Methods: We used a screen-printed technique, 1-step immobilization method, and flow injection technique. We immobilized carcinoembryonic antigen, α-fetoprotein, β-human choriogonadotropin, and carcinoma antigen 125 as model analytes in a redox mediator–grafted, biopolymer-modified, screen-printed carbon electrode array to capture corresponding horseradish peroxidase-labeled antibodies in competitive immunoreactions. The simultaneous multianalyte immunoassay was automatically carried out to amperometrically monitor the mediator-catalyzed enzymatic response to hydrogen peroxide, which decreased in proportion to the concentrations of analytes in samples. Results: The multianalyte immunosensor array had a throughput of 60 samples/h and allowed simultaneous detection of carcinoembryonic antigen, α-fetoprotein, β-human choriogonadotropin, and carcinoma antigen 125 in clinical serum samples with concentrations up to 188 μg/L, 250 μg/L, 266 IU/L, and 334 kIU/L, respectively. The detection limits (limits of the blank, mean of blank plus 3 SD) were 1.1 μg/L, 1.7 μg/L, 1.2 IU/L, and 1.7 kIU/L. The inter- and intraassay imprecision (CVs) of the immunosensor arrays were <7.8% and <9.0%, respectively. The immunosensor arrays were stable for 28 days. Conclusions: This newly constructed immunosensor array provides a simple, automated, simultaneous multianalyte immunoassay with high throughput, short analytical time, and sufficiently low detection limits for clinical application. This method offers the capability of miniaturizing the multianalyte detection device.

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