Covalent enzyme immobilization by poly(ethylene glycol) diglycidyl ether (PEGDE) for microelectrode biosensor preparation

2011 ◽  
Vol 26 (10) ◽  
pp. 3993-4000 ◽  
Author(s):  
Natalia Vasylieva ◽  
Bogdan Barnych ◽  
Anne Meiller ◽  
Caroline Maucler ◽  
Loredano Pollegioni ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Enzyme Immobilization ◽  
Diglycidyl Ether ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

scholarly journals Synthesis of Network Polymers by Means of Addition Reactions of Multifunctional-Amine and Poly(ethylene glycol) Diglycidyl Ether or Diacrylate Compounds

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2047
Author(s):  
Naofumi Naga ◽  
Mitsusuke Sato ◽  
Kensuke Mori ◽  
Hassan Nageh ◽  
Tamaki Nakano
Keyword(s):  
Ethylene Glycol ◽  
Room Temperature ◽  
Addition Reaction ◽  
Monomer Concentration ◽  
Diglycidyl Ether ◽  
Porous Polymers ◽  
Addition Reactions ◽  
Poly Ethylene Glycol ◽  
Network Polymers ◽  
Poly Ethylene

Addition reactions of multi-functional amine, polyethylene imine (PEI) or diethylenetriamine (DETA), and poly(ethylene glycol) diglycidyl ether (PEGDE) or poly(ethylene glycol) diacrylate (PEGDA), have been investigated to obtain network polymers in H2O, dimethyl sulfoxide (DMSO), and ethanol (EtOH). Ring opening addition reaction of the multi-functional amine and PEGDE in H2O at room temperature or in DMSO at 90 °C using triphenylphosphine as a catalyst yielded gels. Aza-Michael addition reaction of the multi-functional amine and PEGDA in DMSO or EtOH at room temperature also yielded corresponding gels. Compression test of the gels obtained with PEI showed higher Young’s modulus than those with DETA. The reactions of the multi-functional amine and low molecular weight PEGDA in EtOH under the specific conditions yielded porous polymers induced by phase separation during the network formation. The morphology of the porous polymers could be controlled by the reaction conditions, especially monomer concentration and feed ratio of the multi-functional amine to PEGDA of the reaction system. The porous structure was formed by connected spheres or a co-continuous monolithic structure. The porous polymers were unbreakable by compression, and their Young’s modulus increased with the increase in the monomer concentration of the reaction systems. The porous polymers absorbed various solvents derived from high affinity between the polyethylene glycol units in the network structure and the solvents.

Poly(Ethylene Glycol) Crosslinked to Albumin as a Support for Enzyme Immobilization

2003 ◽  
pp. 117-124
Author(s):  
Guy Fortier ◽  
Nicole Demers ◽  
Jacques Jean-Franqois ◽  
Jean-Charles Gayet ◽  
Edith M. D'Urso
Keyword(s):  
Ethylene Glycol ◽  
Enzyme Immobilization ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

scholarly journals Toxicity Assessment of a Single Dose of Poly(ethylene glycol) Diglycidyl Ether (PEGDE) Administered Subcutaneously in Mice

Toxics ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 354
Author(s):  
Do-Hyun Kim ◽  
Jong-Hyeon Han ◽  
Hyuk-Cheol Kwon ◽  
Su-Jin Lim ◽  
Seo-Gu Han ◽  
...  
Keyword(s):  
Body Weight ◽  
Ethylene Glycol ◽  
Feed Intake ◽  
Diglycidyl Ether ◽  
Organ Weight ◽  
The Body ◽  
The Other ◽  
Poly Ethylene Glycol ◽  
Weight Variation ◽  
Poly Ethylene

Poly(ethylene glycol) diglycidyl ether (PEGDE) is widely used to cross-link polymers, particularly in the pharmaceutical and biomaterial sectors. However, the subcutaneous toxicity of PEGDE has not yet been assessed. PEGDE samples (500–40,000 μg/mouse) were subcutaneously injected into the paraspinal dorsum of BALB/c male mice. Cage-side observations were carried out with measurement of organ weight, body weight variation, and feed intake, as well as histopathological characterization on day 28 post-exposure. Mice that received 40,000 μg of PEGDE showed severe toxic response and had to be euthanized. Subcutaneous injection of PEGDE did not alter feed intake and organ weight; however, the body weight variation of mice injected with 20,000 μg of PEGDE was significantly lower than that of the other groups. Exposure to 10,000 and 20,000 μg of PEGDE induced epidermal ulcer formation and hair loss. The histology of skin tissue in mice administered with 20,000 μg of PEGDE showed re-epithelialized or unhealed wounds. However, the liver, spleen, and kidneys were histologically normal. Collectively, PEGDE, particularly above 10,000 μg/mouse, caused subcutaneous toxicity with ulceration, but no toxicity in the other organs. These results may indicate the optimal concentration of subcutaneously injected PEGDE.

Photopolymerized Injectable RGD-Modified Fumarated Poly(ethylene glycol) Diglycidyl Ether Hydrogels for Cell Growth

2008 ◽  
Vol 8 (9) ◽  
pp. 852-862 ◽  
Author(s):  
Z. Seden Akdemir ◽  
Handan Akçakaya ◽  
M. Vezir Kahraman ◽  
Taşkın Ceyhan ◽  
Nilhan Kayaman-Apohan ◽  
...  
Keyword(s):  
Cell Growth ◽  
Ethylene Glycol ◽  
Diglycidyl Ether ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene
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