Development of Bio-PORec® system for polyhydroxyalkanoates (PHA) production and its storage in mixed cultures of palm oil mill effluent (POME)

2012 ◽  
Vol 124 ◽  
pp. 208-216 ◽  
Author(s):  
Mohd Fadhil Md. Din ◽  
Ponraj Mohanadoss ◽  
Zaini Ujang ◽  
Mark van Loosdrecht ◽  
Salmiati Muhd Yunus ◽  
...  
2015 ◽  
Vol 72 (11) ◽  
pp. 1889-1895 ◽  
Author(s):  
Tjandra Setiadi ◽  
Martha Aznury ◽  
Azis Trianto ◽  
Adi Pancoro

The highest volatile fatty acids (VFAs) concentration from palm oil mill effluent (POME) treated by anaerobic fermentation was achieved for a 1-day process when the main acids used were acetic, propionic and butyric acids. Polyhydroxyalkanoate (PHA) production with VFAs from POME as precursors in the fed-batch mode has advantages over batch mode, both in terms of its productivity and 3HV (3-hydroxyvalerate) composition in the produced polymer. With the fed batch, the productivity increased to 343% and contained more 3HV than those of the batch. The structures of the PHA were identified by different methods and they supported each other; the resulting products consisted of functional groups of 3HB (3-hydroxybutyrate) and 3HV.


2019 ◽  
Vol 10 (1) ◽  
pp. 4740-4746

Polyhydroxyalkanoates (PHA) are storage granules of most bacteria which can be used as biodegradable plastics but the production cost of PHA is twice than petrochemical based synthetic polymers because of substrate cost. The use of alternative renewable and cheap carbon sources are the best option, one such is palm oil mill effluent (POME). POME contains carbon source like volatile fatty acids and other organic components which can be utilised by microorganisms to accumulate PHA. The use of subinhibitory concentration of antibiotics like clindamycin may have an influence on PHA accumulation. In this study, 31 organisms were isolated from POME spillage area and subjected to PHA production. Seven organisms were found to accumulate PHA, which was confirmed by Nile blue staining method, the accumulated PHA was extracted and characterized using HPLC. All the organisms were found to produced poly hydroxy butyrate (PHB). Amongst all the seven isolates, two organisms namely Bacillus sp and Pseudomonas aeruginosa were found to accumulate more PHA. Both the organisms were subjected to produce PHA in POME and clindamycin containing media. PHA production condition was optimized using RSM.


2015 ◽  
Vol 63 (1) ◽  
pp. 92-100 ◽  
Author(s):  
Mohd Rafais Mohd Razaif-Mazinah ◽  
Mohamad Suffian Mohamad Annuar ◽  
Yusrizam Sharifuddin

Membranes ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 566
Author(s):  
Ruwaida Abdul Wahid ◽  
Wei Lun Ang ◽  
Abdul Wahab Mohammad ◽  
Daniel James Johnson ◽  
Nidal Hilal

Fertilizer-drawn forward osmosis (FDFO) is a potential alternative to recover and reuse water and nutrients from agricultural wastewater, such as palm oil mill effluent that consists of 95% water and is rich in nutrients. This study investigated the potential of commercial fertilizers as draw solution (DS) in FDFO to treat anaerobic palm oil mill effluent (An-POME). The process parameters affecting FO were studied and optimized, which were then applied to fertilizer selection based on FO performance and fouling propensity. Six commonly used fertilizers were screened and assessed in terms of pure water flux (Jw) and reverse salt flux (JS). Ammonium sulfate ((NH4)2SO4), mono-ammonium phosphate (MAP), and potassium chloride (KCl) were further evaluated with An-POME. MAP showed the best performance against An-POME, with a high average water flux, low flux decline, the highest performance ratio (PR), and highest water recovery of 5.9% for a 4-h operation. In a 24-h fouling run, the average flux decline and water recovered were 84% and 15%, respectively. Both hydraulic flushing and osmotic backwashing cleaning were able to effectively restore the water flux. The results demonstrated that FDFO using commercial fertilizers has the potential for the treatment of An-POME for water recovery. Nevertheless, further investigation is needed to address challenges such as JS and the dilution factor of DS for direct use of fertigation.


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