scholarly journals Transcriptome analysis of an oil-rich race B strain of Botryococcus braunii (BOT-70) by de novo assembly of 5′-end sequences of full-length cDNA clones

2012 ◽  
Vol 109 ◽  
pp. 277-281 ◽  
Author(s):  
Motohide Ioki ◽  
Masato Baba ◽  
Nobuyoshi Nakajima ◽  
Yoshihiro Shiraiwa ◽  
Makoto M. Watanabe
Keyword(s):  
Transcriptome Analysis ◽  
De Novo Assembly ◽  
De Novo ◽  
Botryococcus Braunii ◽  
Full Length ◽  
Cdna Clones ◽  
Full Length Cdna ◽  
End Sequences

scholarly journals Infectious Transcripts from Cloned Genome-Length cDNA of Porcine Reproductive and Respiratory Syndrome Virus

1998 ◽  
Vol 72 (1) ◽  
pp. 380-387 ◽  
Author(s):  
J. J. M. Meulenberg ◽  
J. N. A. Bos-de Ruijter ◽  
R. van de Graaf ◽  
G. Wensvoort ◽  
R. J. M. Moormann
Keyword(s):  
Cdna Clone ◽  
De Novo ◽  
Infectious Clone ◽  
Full Length ◽  
Respiratory Syndrome Virus ◽  
Cdna Clones ◽  
Genome Length ◽  
Full Length Cdna ◽  
Lung Macrophages ◽  
Growth Properties

ABSTRACT The 5′-terminal end of the genomic RNA of the Lelystad virus isolate (LV) of porcine reproductive and respiratory syndrome virus was determined. To construct full-length cDNA clones, the 5′-terminal sequence was ligated to cDNA clones covering the complete genome of LV. When RNA that was transcribed in vitro from these full-length cDNA clones was transfected into BHK-21 cells, infectious LV was produced and secreted. The virus was rescued by passage to porcine alveolar lung macrophages or CL2621 cells. When infectious transcripts were transfected to porcine alveolar lung macrophages or CL2621 cells, no infectious virus was produced due to the poor transfection efficiency of these cells. The growth properties of the viruses produced by BHK-21 cells transfected with infectious transcripts of LV cDNA resembled the growth properties of the parental virus from which the cDNA was derived. Two nucleotide changes leading to a unique PacI restriction site directly downstream of the ORF7 gene were introduced in the genome-length cDNA clone. The virus recovered from this mutated cDNA clone retained the PacI site, which confirmed the de novo generation of infectious LV from cloned cDNA. These results indicate that the infectious clone of LV enables us to mutagenize the viral genome at specific sites and that it will therefore be useful for detailed molecular characterization of the virus, as well as for the development of a safe and effective live vaccine for use in pigs.

scholarly journals Transcriptome analysis of an oil-rich race A strain of Botryococcus braunii (BOT-88-2) by de novo assembly of pyrosequencing cDNA reads

2012 ◽  
Vol 109 ◽  
pp. 282-286 ◽  
Author(s):  
Masato Baba ◽  
Motohide Ioki ◽  
Nobuyoshi Nakajima ◽  
Yoshihiro Shiraiwa ◽  
Makoto M. Watanabe
Keyword(s):  
Transcriptome Analysis ◽  
De Novo Assembly ◽  
De Novo ◽  
Botryococcus Braunii

scholarly journals Transcriptome analysis of an oil-rich race B strain of Botryococcus braunii (BOT-22) by de novo assembly of pyrosequencing cDNA reads

2012 ◽  
Vol 109 ◽  
pp. 292-296 ◽  
Author(s):  
Motohide Ioki ◽  
Masato Baba ◽  
Nobuyoshi Nakajima ◽  
Yoshihiro Shiraiwa ◽  
Makoto M. Watanabe
Keyword(s):  
Transcriptome Analysis ◽  
De Novo Assembly ◽  
De Novo ◽  
Botryococcus Braunii

scholarly journals Cost-Effective Sequencing of Full-Length cDNA Clones Powered by a De Novo-Reference Hybrid Assembly

PLoS ONE ◽  
2010 ◽  
Vol 5 (5) ◽  
pp. e10517 ◽  
Author(s):  
Reginaldo M. Kuroshu ◽  
Junichi Watanabe, ◽  
Sumio Sugano ◽  
Shinichi Morishita ◽  
Yutaka Suzuki ◽  
...  
Keyword(s):  
De Novo ◽  
Cost Effective ◽  
Full Length ◽  
Cdna Clones ◽  
Hybrid Assembly ◽  
Full Length Cdna

Cloning and characterization of full-length cDNA clones encoding chalcone synthase from the orchid Bromheadia finlaysoniana

1998 ◽  
Vol 36 (9) ◽  
pp. 647-656 ◽  
Author(s):  
Chye-Fong Liew ◽  
Chong-Jin Goh ◽  
Chiang-Shiong Loh ◽  
Saw-Hoon Lim
Keyword(s):  
Chalcone Synthase ◽  
Full Length ◽  
Cdna Clones ◽  
Full Length Cdna

scholarly journals Large-scale collection of full-length cDNA and transcriptome analysis inHevea brasiliensis

DNA Research ◽  
2017 ◽  
pp. dsw056 ◽  
Author(s):  
Yuko Makita ◽  
Kiaw Kiaw Ng ◽  
G. Veera Singham ◽  
Mika Kawashima ◽  
Hideki Hirakawa ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Large Scale ◽  
Full Length ◽  
Full Length Cdna

scholarly journals Large-scale RT-PCR recovery of full-length cDNA clones

BioTechniques ◽  
2004 ◽  
Vol 36 (4) ◽  
pp. 690-700 ◽  
Author(s):  
Jia Qian Wu ◽  
Angela M. Garcia ◽  
Steven Hulyk ◽  
Anna Sneed ◽  
Carla Kowis ◽  
...  
Keyword(s):  
Large Scale ◽  
Full Length ◽  
Cdna Clones ◽  
Rt Pcr ◽  
Full Length Cdna
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