scholarly journals Construction of Full-Length cDNA Clones to Soil-Borne Wheat Mosaic Virus RNA1 and RNA2, from Which Infectious RNAs Are Transcribed In Vitro: Virion Formation and Systemic Infection without Expression of the N-Terminal and C-Terminal Extensions to the Capsid Protein

Virology ◽  
2000 ◽  
Vol 277 (1) ◽  
pp. 66-75 ◽  
Author(s):  
Aya Yamamiya ◽  
Yukio Shirako
Keyword(s):  
Mosaic Virus ◽  
Capsid Protein ◽  
Systemic Infection ◽  
Full Length ◽  
Cdna Clones ◽  
Full Length Cdna ◽  
Virion Formation

scholarly journals Complete Deletion of Wheat Streak Mosaic Virus HC-Pro: a Null Mutant Is Viable for Systemic Infection

2005 ◽  
Vol 79 (18) ◽  
pp. 12077-12080 ◽  
Author(s):  
Drake C. Stenger ◽  
Roy French ◽  
Frederick E. Gildow
Keyword(s):  
Mosaic Virus ◽  
Virus Titer ◽  
Systemic Infection ◽  
In Vitro Transcription ◽  
Wheat Streak Mosaic Virus ◽  
Null Mutant ◽  
Reverse Transcription Pcr ◽  
Virion Formation ◽  
Insertion Mutations

ABSTRACT A Wheat streak mosaic virus (WSMV) genome lacking HC-Pro was constructed and confirmed by reverse transcription-PCR to systemically infect wheat, oat, and corn. Coupled in vitro transcription/translation reactions indicated that WSMV P1 proteinase cleaved the polyprotein at the P1/P3 junction of the HC-Pro null mutant. The WSMV HC-Pro null mutant was competent for virion formation, but the virus titer was reduced 4.5-fold relative to that of the wild type. Collectively, these results indicate that WSMV HC-Pro is dispensable for replication and movement, two essential processes that are disrupted by point and small-insertion mutations introduced into potyvirus HC-Pro.

Infectivity of in vitro transcripts of Johnsongrass mosaic potyvirus full-length cDNA clones in maize and sorghum

2003 ◽  
Vol 148 (3) ◽  
pp. 563-574 ◽  
Author(s):  
K.-S. Kim ◽  
H.-Y. Oh ◽  
S. Suranto ◽  
E. Nurhayati ◽  
K. H. Gough ◽  
...  
Keyword(s):  
Full Length ◽  
Cdna Clones ◽  
Full Length Cdna ◽  
In Vitro Transcripts

scholarly journals The Capsid Protein of Satellite Panicum Mosaic Virus Contributes to Systemic Invasion and Interacts with Its Helper Virus

2005 ◽  
Vol 79 (15) ◽  
pp. 9756-9764 ◽  
Author(s):  
Rustem T. Omarov ◽  
Dong Qi ◽  
Scholthof G. Karen-Beth
Keyword(s):  
Cell Wall ◽  
Mosaic Virus ◽  
Capsid Protein ◽  
Specific Interaction ◽  
Infected Plant ◽  
Foxtail Millet ◽  
Systemic Infection ◽  
Helper Virus ◽  
Full Length ◽  
Start Codon

ABSTRACT Satellite panicum mosaic virus (SPMV) depends on its helper Panicum mosaic virus (PMV) for replication and spread in host plants. The SPMV RNA encodes a 17-kDa capsid protein (CP) that is essential for formation of its 16-nm virions. The results of this study indicate that in addition to the expression of the full-length SPMV CP from the 5′-proximal AUG start codon, SPMV RNA also expresses a 9.4-kDa C-terminal protein from the third in-frame start codon. Differences in solubility between the full-length protein and its C-terminal product were observed. Subcellular fractionation of infected plant tissues showed that SPMV CP accumulates in the cytosol, cell wall-, and membrane-enriched fractions. However, the 9.4-kDa protein exclusively cofractionated with cell wall- and membrane-enriched fractions. Earlier studies revealed that the 5′-untranslated region (5′-UTR) from nucleotides 63 to 104 was associated with systemic infection in a host-specific manner in millet plants. This study shows that nucleotide deletions and insertions in the 5′-UTR plus simultaneous truncation of the N-terminal part of the CP impaired SPMV spread in foxtail millet, but not in proso millet plants. In contrast, the expression of the full-length version of SPMV CP efficiently compensated the negative effect of the 5′-UTR deletions in foxtail millet. Finally, immunoprecipitation assays revealed the presence of a specific interaction between the capsid proteins of SPMV and its helper virus (PMV). Our findings show that the SPMV CP has several biological functions, including facilitating efficient satellite virus infection and movement in millet plants.

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