Enhanced production of target bioactive metabolites produced by Pseudomonas aeruginosa LV strain

2019 ◽  
Vol 17 ◽  
pp. 545-556 ◽  
Author(s):  
J.C. Bedoya ◽  
M.L. Dealis ◽  
C.S. Silva ◽  
E.T.G. Niekawa ◽  
M.O.P. Navarro ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bioactive Metabolites ◽  
Enhanced Production

Enhanced production of target bioactive metabolites produced by Pseudomonas Aeruginosa LV strain

2019 ◽  
Vol 17 ◽  
pp. 653-664 ◽  
Author(s):  
J.C. Bedoya ◽  
M.L. Dealis ◽  
C.S. Silva ◽  
E.T.G. Niekawa ◽  
M.O.P. Navarro ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bioactive Metabolites ◽  
Enhanced Production

scholarly journals EVALUATION OF DIFFERENT CULTURE MEDIA FOR ENHANCED PRODUCTION OF PSEUDOMONAS AERUGINOSA (MTCC NO 2453) BIOMASS AND ITS PROTEINS

2016 ◽  
Vol 8 (11) ◽  
pp. 120 ◽  
Author(s):  
P. Neeraja ◽  
T. Parthasarathy ◽  
M. Sudhakar
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Protein Production ◽  
Culture Media ◽  
Batch Cultivation ◽  
Luria Bertani ◽  
Shake Flask Culture ◽  
Cell Biomass ◽  
Enhanced Production ◽  
Anti Cancer ◽  
The Media

Objective: Microorganisms, especially bacteria and its proteins have proven to be potential anti-cancer agents as they selectively attack the tumor cells or tumor micro-environments. The extract of Pseudomonas aeruginosa found to contain proteins that have shown promising anticancer activity. In this work, it was attempted to increase the biomass and trigger the total protein fraction of Pseudomonas aeruginosa (MTCC 2453).Methods: The organism was cultivated in three different such as Luria-Bertani (LB) broth, minimal medium9 (M9), super broth medium (SB) and asparagine-proline (AP) broth. Asparagine proline broth was selected as it has shown high cell growth rate. The media was further optimized by the addition of NaHCO3 and copper sulphate to trigger the protein production. Optimized Aspergine proline broth has achieved highest cell biomass. After the shake flask culture, the overnight grown culture in optimized AP medium was further grown in a 5 L bioreactor by fed-batch cultivation to achieve higher cell densities.Results: The highest protein production was achieved at 40 ° C. Highest biomass and protein content was observed at pH 8 while lowest biomass was produced at pH 2. A gradual increase in biomass content observed from 12 h towards to 48 h.Conclusion: High biomass and proteins content and of Pseudomonas aeruginosa (MTCC 2453) can be produced in optimized asparagine-proline broth. Further the extract is purified to produce novel anti-cancer proteins.

scholarly journals Ethanol decreases Pseudomonas aeruginosa flagellar motility through a cyclic-di-GMP- and stator-dependent pathway

2018 ◽  
Author(s):  
Kimberley A. Lewis ◽  
Amy E. Baker ◽  
Annie I. Chen ◽  
Colleen E. Harty ◽  
Sherry L. Kuchma ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Microscopic Analysis ◽  
Fold Increase ◽  
Bioactive Metabolites ◽  
Flagellar Motility ◽  
Planktonic Cells ◽  
Motile Cells ◽  
Diguanylate Cyclases ◽  
Surface Sensing ◽  
In The Wild

AbstractPseudomonas aeruginosa frequently encounters microbes that produce bioactive metabolites including ethanol. At concentrations that do not affect growth, we found that ethanol reduces P. aeruginosa motility by 30% in a swim agar assay and this decrease is accompanied by a 2.5-fold increase in levels of cyclic diguanylate (c-di-GMP), a second messenger that represses motility, in planktonic cells. A screen of mutants lacking genes involved in c-di-GMP metabolism identified SadC and GcbA as diguanylate cyclases involved in swim zone reduction by ethanol and ethanol-induced c-di-GMP production. The reduction of swimming in response to ethanol also required the stator set, MotAB, two PilZ-domain proteins (FlgZ and PilZ), PilY1-a proposed surface-sensing protein, and PilMNOP, which comprises the pilus alignment complex and these proteins have been previously implicated in the control of motility on agar surfaces. Microscopic analysis of the fraction of quiescent cells in swim medium showed that ethanol decreased the portion of motile cells in the wild type, but had opposite effects in the ∆pilY1, ∆pilMNOP, ∆motAB, and ∆pilZ∆flgZ mutants. Together, these data indicate ethanol induces a regulated change in motility in planktonic cells at concentrations similar to those produced by other microbes. We propose that this ethanol-responsiveness may contribute to the co-localization of P. aeruginosa with ethanol-producing microbes.

Conditional changes enhanced production of bioactive metabolites of marine derived fungus Eurotium rubrum

2016 ◽  
Vol 26 (20) ◽  
pp. 4911-4914 ◽  
Author(s):  
Hitoshi Kamauchi ◽  
Kaoru Kinoshita ◽  
Takashi Sugita ◽  
Kiyotaka Koyama
Keyword(s):  
Bioactive Metabolites ◽  
Enhanced Production
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