Conditional changes enhanced production of bioactive metabolites of marine derived fungus Eurotium rubrum

2016 ◽  
Vol 26 (20) ◽  
pp. 4911-4914 ◽  
Author(s):  
Hitoshi Kamauchi ◽  
Kaoru Kinoshita ◽  
Takashi Sugita ◽  
Kiyotaka Koyama
Keyword(s):  
Bioactive Metabolites ◽  
Enhanced Production

Enhanced production of target bioactive metabolites produced by Pseudomonas Aeruginosa LV strain

2019 ◽  
Vol 17 ◽  
pp. 653-664 ◽  
Author(s):  
J.C. Bedoya ◽  
M.L. Dealis ◽  
C.S. Silva ◽  
E.T.G. Niekawa ◽  
M.O.P. Navarro ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bioactive Metabolites ◽  
Enhanced Production

Enhanced production of target bioactive metabolites produced by Pseudomonas aeruginosa LV strain

2019 ◽  
Vol 17 ◽  
pp. 545-556 ◽  
Author(s):  
J.C. Bedoya ◽  
M.L. Dealis ◽  
C.S. Silva ◽  
E.T.G. Niekawa ◽  
M.O.P. Navarro ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bioactive Metabolites ◽  
Enhanced Production

scholarly journals Identification of ε-Poly-L-lysine as an Antimicrobial Product from an Epichloë Endophyte and Isolation of Fungal ε-PL Synthetase Gene

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1032 ◽  
Author(s):  
Enkhee Purev ◽  
Tatsuhiko Kondo ◽  
Daigo Takemoto ◽  
Jennifer T. Niones ◽  
Makoto Ojika
Keyword(s):  
Type Strain ◽  
Plant Pathogens ◽  
Wild Type Strain ◽  
Bioactive Metabolites ◽  
Wild Type ◽  
Enhanced Production ◽  
Enhanced Expression ◽  
In The Wild ◽  
Eukaryotic Genomes ◽  
Epichloë Endophyte

The endophytic fungus Epichloë festucae is known to produce bioactive metabolites, which consequently protect the host plants from biotic and abiotic stresses. We previously found that the overexpression of vibA (a gene for transcription factor) in E. festucae strain E437 resulted in the secretion of an unknown fungicide. In the present study, the active substance was purified and chemically identified as ε-poly-L-lysine (ε-PL), which consisted of 28–34 lysine units. The productivity was 3.7-fold compared with that of the wild type strain E437. The isolated ε-PL showed inhibitory activity against the spore germination of the plant pathogens Drechslera erythrospila, Botrytis cinerea, and Phytophthora infestans at 1–10 μg/mL. We also isolated the fungal gene “epls” encoding ε-PL synthetase Epls. Overexpression of epls in the wild type strain E437 resulted in the enhanced production of ε-PL by 6.7-fold. Interestingly, overexpression of epls in the different strain E. festucae Fl1 resulted in the production of shorter ε-PL with 8–20 lysine, which exhibited a comparable antifungal activity to the longer one. The results demonstrate the first example of ε-PL synthetase gene from the eukaryotic genomes and suggest the potential of enhanced expression of vibA or/and epls genes in the Epichloë endophyte for constructing pest-tolerant plants.

Activation of cytochrome c to a peroxidase compound I-type intermediate by H2O2: relevance to redox signalling in apoptosis

2004 ◽  
Vol 71 ◽  
pp. 97-106 ◽  
Author(s):  
Mark Burkitt ◽  
Clare Jones ◽  
Andrew Lawrence ◽  
Peter Wardman
Keyword(s):  
Cytochrome C ◽  
Hydroxyl Radical ◽  
Redox Regulation ◽  
Chemotherapeutic Agents ◽  
Tyrosyl Radical ◽  
Compound I ◽  
Definitive Evidence ◽  
Enhanced Production ◽  
Physico Chemical

The release of cytochrome c from mitochondria during apoptosis results in the enhanced production of superoxide radicals, which are converted to H2O2 by Mn-superoxide dismutase. We have been concerned with the role of cytochrome c/H2O2 in the induction of oxidative stress during apoptosis. Our initial studies showed that cytochrome c is a potent catalyst of 2′,7′-dichlorofluorescin oxidation, thereby explaining the increased rate of production of the fluorophore 2′,7′-dichlorofluorescein in apoptotic cells. Although it has been speculated that the oxidizing species may be a ferryl-haem intermediate, no definitive evidence for the formation of such a species has been reported. Alternatively, it is possible that the hydroxyl radical may be generated, as seen in the reaction of certain iron chelates with H2O2. By examining the effects of radical scavengers on 2′,7′-dichlorofluorescin oxidation by cytochrome c/H2O2, together with complementary EPR studies, we have demonstrated that the hydroxyl radical is not generated. Our findings point, instead, to the formation of a peroxidase compound I species, with one oxidizing equivalent present as an oxo-ferryl haem intermediate and the other as the tyrosyl radical identified by Barr and colleagues [Barr, Gunther, Deterding, Tomer and Mason (1996) J. Biol. Chem. 271, 15498-15503]. Studies with spin traps indicated that the oxo-ferryl haem is the active oxidant. These findings provide a physico-chemical basis for the redox changes that occur during apoptosis. Excessive changes (possibly catalysed by cytochrome c) may have implications for the redox regulation of cell death, including the sensitivity of tumour cells to chemotherapeutic agents.

Targeted isolation of induced and bioactive metabolites from fungal co-cultures

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
N Bohni ◽  
O Schumpp ◽  
S Schnee ◽  
S Bertrand ◽  
K Gindro ◽  
...  
Keyword(s):  
Bioactive Metabolites

Investigation into the bioactive metabolites of deep sea fungi

Planta Medica ◽  
2015 ◽  
Vol 81 (11) ◽  
Author(s):  
CL Bromley ◽  
RM Young ◽  
S Jackson ◽  
T Sutton ◽  
A Dobson ◽  
...  
Keyword(s):  
Deep Sea ◽  
Bioactive Metabolites
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