Enhanced Production of Biosurfactants by Marine Pseudomonas aeruginosa

2011 ◽  
Vol 46 (1) ◽  
pp. 21-38
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enhanced Production

Enhanced production of target bioactive metabolites produced by Pseudomonas Aeruginosa LV strain

2019 ◽  
Vol 17 ◽  
pp. 653-664 ◽  
Author(s):  
J.C. Bedoya ◽  
M.L. Dealis ◽  
C.S. Silva ◽  
E.T.G. Niekawa ◽  
M.O.P. Navarro ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bioactive Metabolites ◽  
Enhanced Production

scholarly journals EVALUATION OF DIFFERENT CULTURE MEDIA FOR ENHANCED PRODUCTION OF PSEUDOMONAS AERUGINOSA (MTCC NO 2453) BIOMASS AND ITS PROTEINS

2016 ◽  
Vol 8 (11) ◽  
pp. 120 ◽  
Author(s):  
P. Neeraja ◽  
T. Parthasarathy ◽  
M. Sudhakar
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Protein Production ◽  
Culture Media ◽  
Batch Cultivation ◽  
Luria Bertani ◽  
Shake Flask Culture ◽  
Cell Biomass ◽  
Enhanced Production ◽  
Anti Cancer ◽  
The Media

Objective: Microorganisms, especially bacteria and its proteins have proven to be potential anti-cancer agents as they selectively attack the tumor cells or tumor micro-environments. The extract of Pseudomonas aeruginosa found to contain proteins that have shown promising anticancer activity. In this work, it was attempted to increase the biomass and trigger the total protein fraction of Pseudomonas aeruginosa (MTCC 2453).Methods: The organism was cultivated in three different such as Luria-Bertani (LB) broth, minimal medium9 (M9), super broth medium (SB) and asparagine-proline (AP) broth. Asparagine proline broth was selected as it has shown high cell growth rate. The media was further optimized by the addition of NaHCO3 and copper sulphate to trigger the protein production. Optimized Aspergine proline broth has achieved highest cell biomass. After the shake flask culture, the overnight grown culture in optimized AP medium was further grown in a 5 L bioreactor by fed-batch cultivation to achieve higher cell densities.Results: The highest protein production was achieved at 40 ° C. Highest biomass and protein content was observed at pH 8 while lowest biomass was produced at pH 2. A gradual increase in biomass content observed from 12 h towards to 48 h.Conclusion: High biomass and proteins content and of Pseudomonas aeruginosa (MTCC 2453) can be produced in optimized asparagine-proline broth. Further the extract is purified to produce novel anti-cancer proteins.

scholarly journals Pseudomonas aeruginosa relA Contributes to Virulence in Drosophila melanogaster

2004 ◽  
Vol 72 (10) ◽  
pp. 5638-5645 ◽  
Author(s):  
David L. Erickson ◽  
J. Louise Lines ◽  
Everett C. Pesci ◽  
Vittorio Venturi ◽  
Douglas G. Storey
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Stringent Response ◽  
Laboratory Strain ◽  
Homoserine Lactone ◽  
Nutritional Deficiencies ◽  
Low Magnesium ◽  
Sensing Systems ◽  
Enhanced Production ◽  
Cystic Fibrosis Isolate

ABSTRACT The stringent response is a mechanism by which bacteria adapt to nutritional deficiencies through the production of the guanine nucleotides ppGpp and pppGpp, produced by the RelA enzyme. We investigated the role of the relA gene in the ability of an extracellular pathogen, Pseudomonas aeruginosa, to cause infection. Strains lacking the relA gene were created from the prototypical laboratory strain PAO1 as well as the mucoid cystic fibrosis isolate 6106, which lacks functional quorum-sensing systems. The absence of relA abolished the production of ppGpp and pppGpp under conditions of amino acid starvation. We found that strains lacking relA exhibited reduced virulence in a D. melanogaster feeding assay. In conditions of low magnesium, the relA gene enhanced production of the cell-cell signal N-[3-oxododecanoyl]-l-homoserine lactone, whereas relA reduced the production of the 2-heptyl-3-hydroxy-4-quinolone signal during serine hydroxamate induction of the stringent response. In the relA mutant, alterations in the Pseudomonas quinolone system pathways seemed to increase the production of pyocyanin and decrease the production of elastase. Deletion of relA also resulted in reduced levels of the RpoS sigma factor. These results suggest that adjustment of cellular ppGpp and pppGpp levels could be an important regulatory mechanism in P. aeruginosa adaptation in pathogenic relationships.

Enhanced production of target bioactive metabolites produced by Pseudomonas aeruginosa LV strain

2019 ◽  
Vol 17 ◽  
pp. 545-556 ◽  
Author(s):  
J.C. Bedoya ◽  
M.L. Dealis ◽  
C.S. Silva ◽  
E.T.G. Niekawa ◽  
M.O.P. Navarro ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bioactive Metabolites ◽  
Enhanced Production
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