Volume regulation of murine T lymphocytes relies on voltage-dependent and two-pore domain potassium channels

2011 ◽  
Vol 1808 (8) ◽  
pp. 2036-2044 ◽  
Author(s):  
Nicole Bobak ◽  
Stefan Bittner ◽  
Joseph Andronic ◽  
Susanne Hartmann ◽  
Friederike Mühlpfordt ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Potassium Channels ◽  
Volume Regulation ◽  
Voltage Dependent ◽  
Pore Domain

scholarly journals Identification of two-pore domain potassium channels as potent modulators of osmotic volume regulation in human T lymphocytes

2013 ◽  
Vol 1828 (2) ◽  
pp. 699-707 ◽  
Author(s):  
Joseph Andronic ◽  
Nicole Bobak ◽  
Stefan Bittner ◽  
Petra Ehling ◽  
Christoph Kleinschnitz ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Potassium Channels ◽  
Volume Regulation ◽  
Human T Lymphocytes ◽  
Pore Domain

scholarly journals Effect of Malnutrition on K+ Current in T Lymphocytes

2005 ◽  
Vol 12 (7) ◽  
pp. 808-813 ◽  
Author(s):  
Rafael Godínez Fernández ◽  
Joaquín Azpiroz Leehan ◽  
Reyna Fierro Pastrana ◽  
Rocío Ortíz Muñiz
Keyword(s):  
Membrane Potential ◽  
T Lymphocytes ◽  
Animal Models ◽  
Potassium Channels ◽  
Infectious Diseases ◽  
Severe Malnutrition ◽  
Voltage Dependent ◽  
K Current

ABSTRACT Severe malnutrition in children is frequently associated with infectious diseases. Animal models have been useful for studying the effects of malnutrition. One of the immunosuppressive mechanisms of malnutrition is inhibition of the activation of T lymphocytes. The voltage-dependent K(V) potassium channels are vital for the activation of T lymphocytes. The blockade of K(V) channels inhibits the activation of T lymphocytes. Malnutrition could affect the suitable synthesis of K(V) channels in T lymphocytes, producing changes in the magnitude and/or dependency of the voltage of the K+ current. We reported a significant decrease in the K+ current and activation to a 20 mV more positive membrane potential in T lymphocytes of rats with severe malnutrition. These results indicate that the diminution in the K+ conductance by alteration of K(V) channels in severe malnutrition is one of the mechanisms that inhibit the activation of T lymphocytes.

scholarly journals Interaction between tetraethylammonium and amino acid residues in the pore of cloned voltage-dependent potassium channels.

1991 ◽  
Vol 266 (12) ◽  
pp. 7583-7587
Author(s):  
M P Kavanaugh ◽  
M D Varnum ◽  
P B Osborne ◽  
M J Christie ◽  
A E Busch ◽  
...  
Keyword(s):  
Amino Acid ◽  
Potassium Channels ◽  
Amino Acid Residues ◽  
Voltage Dependent

Differential expression of two-pore domain potassium channels in rat cerebellar granule neurons

2014 ◽  
Vol 453 (4) ◽  
pp. 754-760 ◽  
Author(s):  
Paulina Burgos ◽  
Rafael Zúñiga ◽  
Pedro Domínguez ◽  
Fernando Delgado-López ◽  
Leigh D. Plant ◽  
...  
Keyword(s):  
Potassium Channels ◽  
Differential Expression ◽  
Cerebellar Granule Neurons ◽  
Granule Neurons ◽  
Cerebellar Granule ◽  
Pore Domain

scholarly journals Activation-inactivation of potassium channels and development of the potassium-channel spike in internally perfused squid giant axons.

1981 ◽  
Vol 78 (1) ◽  
pp. 43-61 ◽  
Author(s):  
I Inoue
Keyword(s):  
Potassium Channels ◽  
Potassium Channel ◽  
Voltage Clamp ◽  
Time Constant ◽  
Equilibrium Potential ◽  
Giant Axons ◽  
Dependent Inactivation ◽  
Voltage Dependent ◽  
Time Courses ◽  
Calcium Permeability

A spike that is the result of calcium permeability through potassium channels was separated from the action potential is squid giant axons internally perfused with a 30 mM NaF solution and bathed in a 100 mM CaCl2 solution by blocking sodium channels with tetrodotoxin. Currents through potassium channels were studied under voltage clamp. The records showed a clear voltage-dependent inactivation of the currents. The inactivation was composed of at least two components; one relatively fast, having a time constant of 20--30 ms, and the other very slow, having a time constant of 5--10 s. Voltage clamp was carried out with a variety of salt compositions in both the internal and external solutions. A similar voltage-dependent inactivation, also composed of the two components, was recognized in all the current through potassium channels. Although the direction and intensity of current strongly depended on the salt composition of the solutions, the time-courses of these currents at corresponding voltages were very similar. These results strongly suggest that the inactivation of the currents in attributable to an essential, dynamic property of potassium channels themselves. Thus, the generation of a potassium-channel spike can be understood as an event that occurs when the equilibrium potential across the potassium channel becomes positive.

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