Fabrication of dopamine-modified hyaluronic acid/chitosan multilayers on titanium alloy by layer-by-layer self-assembly for promoting osteoblast growth

2013 ◽  
Vol 284 ◽  
pp. 732-737 ◽  
Author(s):  
Xinming Zhang ◽  
Zhaoyang Li ◽  
Xubo Yuan ◽  
Zhenduo Cui ◽  
Xianjin Yang
Keyword(s):  
Titanium Alloy ◽  
Hyaluronic Acid ◽  
Self Assembly ◽  
Layer By Layer

scholarly journals Osteocytes Influence on Bone Matrix Integrity Affects Biomechanical Competence at Bone-Implant Interface of Bioactive-Coated Titanium Implants in Rat Tibiae

2021 ◽  
Vol 23 (1) ◽  
pp. 374
Author(s):  
Sabine Stoetzel ◽  
Deeksha Malhan ◽  
Ute Wild ◽  
Christian Helbing ◽  
Fathi Hassan ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Titanium Alloy ◽  
Hyaluronic Acid ◽  
Self Assembly ◽  
Bone Matrix ◽  
Preclinical Study ◽  
Titanium Implants ◽  
Layer By Layer ◽  
Bioactive Coatings ◽  
Bone Interface

Osseointegration is a prerequisite for the long-term success of implants. Titanium implants are preferred for their biocompatibility and mechanical properties. Nonetheless, the need for early and immediate loading requires enhancing these properties by adding bioactive coatings. In this preclinical study, extracellular matrix properties and cellular balance at the implant/bone interface was examined. Polyelectrolyte multilayers of chitosan and gelatin or with chitosan and Hyaluronic acid fabricated on titanium alloy using a layer-by-layer self-assembly process were compared with native titanium alloy. The study aimed to histologically evaluate bone parameters that correlate to the biomechanical anchorage enhancement resulted from bioactive coatings of titanium implants in a rat animal model. Superior collagen fiber arrangements and an increased number of active osteocytes reflected a significant improvement of bone matrix quality at the bone interface of the chitosan/gelatin-coated titan implants over chitosan/hyaluronic acid-coated and native implants. Furthermore, the numbers and localization of osteoblasts and osteoclasts in the reparative and remodeling phases suggested a better cellular balance in the chitosan/Gel-coated group over the other two groups. Investigating the micro-mechanical properties of bone tissue at the interface can elucidate detailed discrepancies between different promising bioactive coatings of titanium alloys to maximize their benefit in future medical applications.

Salivary polypeptide/hyaluronic acid multilayer coatings act as “fungal repellents” and prevent biofilm formation on biomaterials

2018 ◽  
Vol 6 (10) ◽  
pp. 1452-1457 ◽  
Author(s):  
Jianchuan Wen ◽  
Chih-Ko Yeh ◽  
Yuyu Sun
Keyword(s):  
Hyaluronic Acid ◽  
Self Assembly ◽  
Biofilm Formation ◽  
Design Principle ◽  
Multilayer Coatings ◽  
Layer By Layer ◽  
Denture Stomatitis ◽  
Polyelectrolyte Layer ◽  
Assembly Technology ◽  
Systemic Health

Candida-associated denture stomatitis (CADS) is a common, recurring clinical complication in denture wearers that can lead to serious oral and systemic health problems. Polyelectrolyte layer-by-layer (LBL) self-assembly technology on denture materials offers a new design principle for controlling fungal biofilm formation.

scholarly journals Fabrication and in vitro evaluation of stable collagen/hyaluronic acid biomimetic multilayer on titanium coatings

2013 ◽  
Vol 10 (84) ◽  
pp. 20130070 ◽  
Author(s):  
Haiyong Ao ◽  
Youtao Xie ◽  
Honglue Tan ◽  
Shengbing Yang ◽  
Kai Li ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Photoelectron Spectroscopy ◽  
Self Assembly ◽  
Human Mesenchymal Stem Cells ◽  
Biological Properties ◽  
Covalent Immobilization ◽  
Layer By Layer ◽  
Titanium Coating ◽  
Assembly Technique

Layer-by-layer (LBL) self-assembly technique has been proved to be a highly effective method to immobilize the main components of the extracellular matrix such as collagen and hyaluronic acid on titanium-based implants and form a polyelectrolyte multilayer (PEM) film by electrostatic interaction. However, the formed PEM film is unstable in the physiological environment and affects the long-time effectiveness of PEM film. In this study, a modified LBL technology has been developed to fabricate a stable collagen/hyaluronic acid (Col/HA) PEM film on titanium coating (TC) by introducing covalent immobilization. Scanning electron microscopy, diffuse reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy were used to characterize the PEM film. Results of Sirius red staining demonstrated that the chemical stability of PEM film was greatly improved by covalent cross-linking. Cell culture assays further illustrated that the functions of human mesenchymal stem cells, such as attachment, spreading, proliferation and differentiation, were obviously enhanced by the covalently immobilized Col/HA PEM on TCs compared with the absorbed Col/HA PEM. The improved stability and biological properties of the Col/HA PEM covalently immobilized TC may be beneficial to the early osseointegration of the implants.

Layer-by-Layer Thin Films of Alginate/Chitosan and Hyaluronic Acid/Chitosan with Tunable Thickness and Surface Roughness

2014 ◽  
Vol 783-786 ◽  
pp. 1226-1231 ◽  
Author(s):  
Thiago Bezerra Taketa ◽  
Marisa Masumi Beppu
Keyword(s):  
Thin Films ◽  
Hyaluronic Acid ◽  
Self Assembly ◽  
Polymer Chains ◽  
Layer By Layer ◽  
Natural Polymers ◽  
Force Microscopy ◽  
Atomic Force ◽  
Polyelectrolyte Solutions ◽  
Oppositely Charged

Layer-by-layer (LbL) is a bottom-up technique used for construction of films with self-assembly and self-organizing properties. In most cases, the fundamental driving force for the formation of these films is originated from the electrostatic interaction between oppositely charged species. The charged segments of polyelectrolytes behave as small building units and their orientation and position can be designed to target structures of great complexity. Furthermore, the technique enables the use of various materials, including natural polymers. In this work, we chose the cationic biopolymer chitosan (CHI) and the negative polyelectrolytes sodium alginate (ALG) and hyaluronic acid (HA). The aim of this study was to evaluate the effect of ionic strength (0 versus 200 mM) and pH (3 versus 5) on ALG/CHI and HA/CHI nanostructured multilayered thin films properties. From profilometry and atomic force microscopy (AFM) analyses, changes in thickness and roughness of the coatings were monitored. The presence of salt in polyelectrolyte solutions induced the polymer chains to adopt conformations with more loops and tails and this arrangement in solution was transmitted to films, resulting in rougher surfaces. Furthermore, the film thickness can be precisely controlled by adjusting the pH of the polyelectrolyte solution. The variation of these parameters shows that it is possible to molecularly control chemical and structural properties of nanostructured coatings, thus opening up new possibilities of application (e.g. cell adhesion).

Layer-by-Layer Self-Assembly of Modified Hyaluronic Acid/Chitosan Based on Hydrogen Bonding

2009 ◽  
Vol 10 (9) ◽  
pp. 2632-2639 ◽  
Author(s):  
Uttam Manna ◽  
Sri Bharani ◽  
Satish Patil
Keyword(s):  
Hydrogen Bonding ◽  
Hyaluronic Acid ◽  
Self Assembly ◽  
Layer By Layer
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