Salivary polypeptide/hyaluronic acid multilayer coatings act as “fungal repellents” and prevent biofilm formation on biomaterials

2018 ◽  
Vol 6 (10) ◽  
pp. 1452-1457 ◽  
Author(s):  
Jianchuan Wen ◽  
Chih-Ko Yeh ◽  
Yuyu Sun
Keyword(s):  
Hyaluronic Acid ◽  
Self Assembly ◽  
Biofilm Formation ◽  
Design Principle ◽  
Multilayer Coatings ◽  
Layer By Layer ◽  
Denture Stomatitis ◽  
Polyelectrolyte Layer ◽  
Assembly Technology ◽  
Systemic Health

Candida-associated denture stomatitis (CADS) is a common, recurring clinical complication in denture wearers that can lead to serious oral and systemic health problems. Polyelectrolyte layer-by-layer (LBL) self-assembly technology on denture materials offers a new design principle for controlling fungal biofilm formation.

scholarly journals Construction of Chitosan/Alginate Nano-Drug Delivery System for Improving Dextran Sodium Sulfate-Induced Colitis in Mice

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1884
Author(s):  
Mengfei Jin ◽  
Shangyong Li ◽  
Yanhong Wu ◽  
Dandan Li ◽  
Yantao Han
Keyword(s):  
Self Assembly ◽  
Average Diameter ◽  
Mucosal Healing ◽  
Plga Nanoparticles ◽  
Narrow Particle Size Distribution ◽  
Layer By Layer ◽  
Systemic Side Effects ◽  
Assembly Technology ◽  
The Fourier Transform ◽  
Polyelectrolyte Film

(1) Background: In the treatment of ulcerative colitis (UC), accurate delivery and release of anti-inflammatory drugs to the site of inflammation can reduce systemic side effects. (2) Methods: We took advantage of this goal to prepare resveratrol-loaded PLGA nanoparticles (RES-PCAC-NPs) by emulsification solvent volatilization. After layer-by-layer self-assembly technology, we deposited chitosan and alginate to form a three-layer polyelectrolyte film. (3) Results: It can transport nanoparticles through the gastric environment to target inflammation sites and slowly release drugs at a specific pH. The resulting RES-PCAC-NPs have an ideal average diameter (~255 nm), a narrow particle size distribution and a positively charged surface charge (~13.5 mV). The Fourier transform infrared spectroscopy showed that resveratrol was successfully encapsulated into PCAC nanoparticles, and the encapsulation efficiency reached 87.26%. In addition, fluorescence imaging showed that RES-PCAC-NPs with positive charges on the surface can effectively target and accumulate in the inflammation site while continuing to penetrate downward to promote mucosal healing. Importantly, oral RES-PCAC-NPs treatment in DSS-induced mice was superior to other results in significantly improved inflammatory markers of UC. (4) Conclusions: Our results strongly prove that RES-PCAC-NPs can target the inflamed colon for maximum efficacy, and this oral pharmaceutical formulation can represent a promising formulation in the treatment of UC.

scholarly journals Fabrication and in vitro evaluation of stable collagen/hyaluronic acid biomimetic multilayer on titanium coatings

2013 ◽  
Vol 10 (84) ◽  
pp. 20130070 ◽  
Author(s):  
Haiyong Ao ◽  
Youtao Xie ◽  
Honglue Tan ◽  
Shengbing Yang ◽  
Kai Li ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Photoelectron Spectroscopy ◽  
Self Assembly ◽  
Human Mesenchymal Stem Cells ◽  
Biological Properties ◽  
Covalent Immobilization ◽  
Layer By Layer ◽  
Titanium Coating ◽  
Assembly Technique

Layer-by-layer (LBL) self-assembly technique has been proved to be a highly effective method to immobilize the main components of the extracellular matrix such as collagen and hyaluronic acid on titanium-based implants and form a polyelectrolyte multilayer (PEM) film by electrostatic interaction. However, the formed PEM film is unstable in the physiological environment and affects the long-time effectiveness of PEM film. In this study, a modified LBL technology has been developed to fabricate a stable collagen/hyaluronic acid (Col/HA) PEM film on titanium coating (TC) by introducing covalent immobilization. Scanning electron microscopy, diffuse reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy were used to characterize the PEM film. Results of Sirius red staining demonstrated that the chemical stability of PEM film was greatly improved by covalent cross-linking. Cell culture assays further illustrated that the functions of human mesenchymal stem cells, such as attachment, spreading, proliferation and differentiation, were obviously enhanced by the covalently immobilized Col/HA PEM on TCs compared with the absorbed Col/HA PEM. The improved stability and biological properties of the Col/HA PEM covalently immobilized TC may be beneficial to the early osseointegration of the implants.

Preparation and Characterization of Nano-Films Materials

2011 ◽  
Vol 492 ◽  
pp. 160-163
Author(s):  
Cai Xia Li ◽  
Qing Lv ◽  
Jie Song ◽  
Dan Yu Jiang ◽  
Qiang Li
Keyword(s):  
Self Assembly ◽  
Chemical Properties ◽  
Layered Compounds ◽  
Insertion Reaction ◽  
Layer By Layer ◽  
Vis Spectroscopy ◽  
Assembly Technique ◽  
Assembly Technology ◽  
Physical And Chemical

Nano-sheets are two-dimensional sheet materials exfoliated from the inorganic layered compounds by various physical and chemical methods. Their unique characteristics insertion reaction and excellent physical and chemical properties have attracted more and more researchers' widespread interests. Selecting quartz glass as the substrate, using layer by layer self-assembly technology, different nano-films materials are prepared. UV/Vis spectroscopy confirmed nano-films materials have been successfully assembled using LBL self-assembly technique. Raman spectrum are mainly used to analyze and characterize the structure of nano-films materials.

UV-Enhanced Bioactivity of PS/TiO2 Coating Prepared by Layer-by-Layer Self-Assembly Method

2010 ◽  
Vol 434-435 ◽  
pp. 579-581
Author(s):  
Zhi Xian Zhang ◽  
Fan Xiao ◽  
Fei Gao
Keyword(s):  
Uv Irradiation ◽  
Ultraviolet Irradiation ◽  
Self Assembly ◽  
Raw Material ◽  
Layer By Layer ◽  
Titanium Coating ◽  
Water Contact ◽  
Oh Groups ◽  
Assembly Method ◽  
Assembly Technology

The TiO2 nanopowder was prepared with TiOSO4 as raw material at low temperature by hydrolysis method, and microspheres coated polyethylene and TiO2 were then fabricated on titanium by layer-by-layer self-assembly technology. Using ultraviolet irradiation of titanium coating in distilled water for some time, titanium with enhanced bioactivity was achieved when immersed in simulated body fluid (SBF). The morphology, size and crystal shape before and after ultraviolet irradiation were characterized by scanning electron microcopy (SEM) and X-ray diffraction (XRD). UV irradiation of titanium results in the conversion of Ti4+ to Ti3+ and the generation of oxygen vacancies, which could react with the absorbed water to form basic Ti–OH groups. Compared with the coating non-UV irradiation, the UV-irradiated coatings do not present any obvious differences in morphology, surface roughness, grain size and phase component; however, they have more abundant basic Ti–OH groups thus the water contact angle decreases greatly so the surface become much more hydrophilic.

Bioactivity of L-b-L Self-Assembly TiO2 Hollow Spheres by UV Irradiation Enhanced

2011 ◽  
Vol 328-330 ◽  
pp. 1495-1498
Author(s):  
Fan Xiao ◽  
Wei Li ◽  
Fei Gao ◽  
Zhi Xian Zhang
Keyword(s):  
Simulated Body Fluid ◽  
Uv Irradiation ◽  
Self Assembly ◽  
Body Fluid ◽  
Irradiation Time ◽  
Hollow Spheres ◽  
Layer By Layer ◽  
Assembly Technology ◽  
Hydrolysis Of ◽  
Better Than

Using nano-TiO2 particles obtained by hydrolysis of titanium oxysulfate (TiOSO4) as the precursor, and polystyrene (PS) spheres as the template, TiO2 hollow spheres were successfully deposited on CPTi substrates through layer-by-layer self-assembly technology. After ultraviolet light (UV) irradiation, the TiO2 coatings were soaked in simulated body fluid (SBF) to explore their bioactivities. The effects of UV irradiation time and UV irradiation media on the bioactivities of nano TiO2 coatings were investigated. The results show that bone-like apatite can be formed on the surface of nano TiO2 coating irradiated by UV, and this reveals that the irradiated coating has good bioactivity. The amount of apatite formed on the surface of the coating increases with the increase of UV irradiation time. UV-irradiated media also influence the bioactivity of the coating. The bioactivities of nano TiO2 coatings irradiated in air and SBF are better than that in water.

Layer-by-Layer Thin Films of Alginate/Chitosan and Hyaluronic Acid/Chitosan with Tunable Thickness and Surface Roughness

2014 ◽  
Vol 783-786 ◽  
pp. 1226-1231 ◽  
Author(s):  
Thiago Bezerra Taketa ◽  
Marisa Masumi Beppu
Keyword(s):  
Thin Films ◽  
Hyaluronic Acid ◽  
Self Assembly ◽  
Polymer Chains ◽  
Layer By Layer ◽  
Natural Polymers ◽  
Force Microscopy ◽  
Atomic Force ◽  
Polyelectrolyte Solutions ◽  
Oppositely Charged

Layer-by-layer (LbL) is a bottom-up technique used for construction of films with self-assembly and self-organizing properties. In most cases, the fundamental driving force for the formation of these films is originated from the electrostatic interaction between oppositely charged species. The charged segments of polyelectrolytes behave as small building units and their orientation and position can be designed to target structures of great complexity. Furthermore, the technique enables the use of various materials, including natural polymers. In this work, we chose the cationic biopolymer chitosan (CHI) and the negative polyelectrolytes sodium alginate (ALG) and hyaluronic acid (HA). The aim of this study was to evaluate the effect of ionic strength (0 versus 200 mM) and pH (3 versus 5) on ALG/CHI and HA/CHI nanostructured multilayered thin films properties. From profilometry and atomic force microscopy (AFM) analyses, changes in thickness and roughness of the coatings were monitored. The presence of salt in polyelectrolyte solutions induced the polymer chains to adopt conformations with more loops and tails and this arrangement in solution was transmitted to films, resulting in rougher surfaces. Furthermore, the film thickness can be precisely controlled by adjusting the pH of the polyelectrolyte solution. The variation of these parameters shows that it is possible to molecularly control chemical and structural properties of nanostructured coatings, thus opening up new possibilities of application (e.g. cell adhesion).

Layer-by-layer self-assembly of minocycline-loaded chitosan/alginate multilayer on titanium substrates to inhibit biofilm formation

2014 ◽  
Vol 42 (11) ◽  
pp. 1464-1472 ◽  
Author(s):  
Hongbin Lv ◽  
Zhen Chen ◽  
Xiaoping Yang ◽  
Lian Cen ◽  
Xu Zhang ◽  
...  
Keyword(s):  
Self Assembly ◽  
Biofilm Formation ◽  
Layer By Layer ◽  
Titanium Substrates
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