Injectable decellularized nucleus pulposus-based cell delivery system for differentiation of adipose-derived stem cells and nucleus pulposus regeneration

2018 ◽  
Vol 81 ◽  
pp. 115-128 ◽  
Author(s):  
Xiaopeng Zhou ◽  
Jingkai Wang ◽  
Xianpeng Huang ◽  
Weijing Fang ◽  
Yiqing Tao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Nucleus Pulposus ◽  
Delivery System ◽  
Cell Delivery ◽  
Adipose Derived Stem Cells

Development of a cell delivery system using alginate microbeads for tissue regeneration

2016 ◽  
Vol 4 (20) ◽  
pp. 3515-3525 ◽  
Author(s):  
Shirae K. Leslie ◽  
Anthony M. Nicolini ◽  
Gobalakrishnan Sundaresan ◽  
Jamal Zweit ◽  
Barbara D. Boyan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Delivery System ◽  
Tissue Regeneration ◽  
Cell Delivery ◽  
Adipose Derived Stem Cells ◽  
Viable Cells ◽  
A Cell

Alginate microbeads incorporating adipose-derived stem cells (ASCs) have potential for delivering viable cells capable of facilitating tissue regeneration.

scholarly journals Genipin Cross-Linked Decellularized Nucleus Pulposus Hydrogel-Like Cell Delivery System Induces Differentiation of ADSCs and Retards Intervertebral Disc Degeneration

2021 ◽  
Vol 9 ◽  
Author(s):  
Lei Yu ◽  
Yi Liu ◽  
Jianxin Wu ◽  
Shuang Wang ◽  
Jiangming Yu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Delivery System ◽  
Intervertebral Disc Degeneration ◽  
Therapeutic Potential ◽  
Cell Delivery ◽  
Intervertebral Disc Regeneration

Intervertebral disc degeneration (IDD) is the pathological basis of disc degenerative diseases (DDD). Reduction in the number of cells and degeneration of the extracellular matrix (ECM) in the nucleus pulposus (NP) are characteristics of IDD. Bio-hydrogel combined with stem cell transplantation is a promising treatment. Injectable ECM hydrogels have good biological activity and in-situ gelatinization. However, its biomechanics and stability are insufficient to provide adequate mechanical support for intervertebral discs and to maintain the long-term differential stimulus for seeded stem cells. In our study, we developed genipin cross-linked decellularized nucleus pulposus hydrogel (GDH) as delivery system. We evaluated the mechanical properties, stability, biocompatibility, and differentiation induction of GDH cross-linked with different concentrations of genipin in vitro. The GDH-loaded adipose-derived mesenchymal stem cells (ADSCs) (GDHA) were injected into the rat degenerated coccygeal intervertebral disc. The effect of intervertebral disc regeneration in vivo was evaluated. The results showed that GDH with 0.02% of genipin had similar elastic modulus to human nucleus pulposus, good biocompatibility, and inducibility of expressing NP-related genes. In vivo studies showed that GDHA improved the survival of ADSCs and improved the intervertebral height, MRI index, and histological grading score. In conclusion, GDH, as an outstanding bio-hydrogel cell delivery system, has the therapeutic potential for retarding IDD.

scholarly journals Multi-modal imaging probe for assessing the efficiency of stem cell delivery to orthotopic breast tumours

Nanoscale ◽  
2020 ◽  
Vol 12 (31) ◽  
pp. 16570-16585 ◽  
Author(s):  
May Zaw Thin ◽  
Helen Allan ◽  
Robin Bofinger ◽  
Tomas D. Kostelec ◽  
Simon Guillaume ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Delivery ◽  
Adipose Derived Stem Cells ◽  
Breast Tumours ◽  
Imaging Probe ◽  
Stem Cell Delivery

Illustration of adipose-derived stem cells with tri-modal imaging capabilities for evaluating the efficiency of cell delivery to tumours.

Design of a Bioreactor for Mechanical Stimulation of Adipose Derived Stem Cells for Intervertebral Disc Tissue Engineering

2012 ◽  
Author(s):  
Heather Cleary ◽  
Thomas Barkley ◽  
Adam Goodman ◽  
Michael Payne ◽  
John Virtue ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Compressive Loading ◽  
Cartilage Regeneration ◽  
Adipose Derived Stem Cells ◽  
Medical Problems ◽  
Tissue Engineering Scaffolds ◽  
Engineering Strategy

Lower back pain is one of the most common medical problems in the world [1], affecting between 70% and 85% of the US population at some point during their lives [2]. Disc degeneration is caused by biological changes in the disc, which result in dehydration of the nucleus pulposus (NP). The long term goal of this project is to treat disc degeneration with a tissue engineering strategy for the regeneration of the nucleus pulposus using messechymal stem cells derived from adipose tissue. It has been established in cartilage regeneration studies that cyclic compressive loading of stem cells is beneficial for tissue formation compared to static culture [3–7]. In this work, a bioreactor is being developed that can subject cell-seeded polymeric tissue engineering scaffolds to dynamic compressive forces. Ultimately, the bioreactor will be used to study the effects of different loading parameters on the production of new nucleus pulposus tissue from adipose-derived stem cells.

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