Design of a Bioreactor for Mechanical Stimulation of Adipose Derived Stem Cells for Intervertebral Disc Tissue Engineering

2012 ◽  
Author(s):  
Heather Cleary ◽  
Thomas Barkley ◽  
Adam Goodman ◽  
Michael Payne ◽  
John Virtue ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Compressive Loading ◽  
Cartilage Regeneration ◽  
Adipose Derived Stem Cells ◽  
Medical Problems ◽  
Tissue Engineering Scaffolds ◽  
Engineering Strategy

Lower back pain is one of the most common medical problems in the world [1], affecting between 70% and 85% of the US population at some point during their lives [2]. Disc degeneration is caused by biological changes in the disc, which result in dehydration of the nucleus pulposus (NP). The long term goal of this project is to treat disc degeneration with a tissue engineering strategy for the regeneration of the nucleus pulposus using messechymal stem cells derived from adipose tissue. It has been established in cartilage regeneration studies that cyclic compressive loading of stem cells is beneficial for tissue formation compared to static culture [3–7]. In this work, a bioreactor is being developed that can subject cell-seeded polymeric tissue engineering scaffolds to dynamic compressive forces. Ultimately, the bioreactor will be used to study the effects of different loading parameters on the production of new nucleus pulposus tissue from adipose-derived stem cells.

scholarly journals Auricular Cartilage Regeneration with Adipose-Derived Stem Cells in Rabbits

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Se-Joon Oh ◽  
Hee-Young Park ◽  
Kyung-Un Choi ◽  
Sung-Won Choi ◽  
Sung-Dong Kim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cartilage Defect ◽  
Collagen Type ◽  
Cartilage Regeneration ◽  
Control Group ◽  
Adipose Derived Stem Cells ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Auricular Cartilage

Tissue engineering cell-based therapy using induced pluripotent stem cells and adipose-derived stem cells (ASCs) may be promising tools for therapeutic applications in tissue engineering because of their abundance, relatively easy harvesting, and high proliferation potential. The purpose of this study was to investigate whether ASCs can promote the auricular cartilage regeneration in the rabbit. In order to assess their differentiation ability, ASCs were injected into the midportion of a surgically created auricular cartilage defect in the rabbit. Control group was injected with normal saline. After 1 month, the resected auricles were examined histopathologically and immunohistochemically. The expression of collagen type II and transforming growth factor-β1 (TGF-β1) were analyzed by quantitative polymerase chain reaction. Histopathology showed islands of new cartilage formation at the site of the surgically induced defect in the ASC group. Furthermore, Masson’s trichrome staining and immunohistochemistry for S-100 showed numerous positive chondroblasts. The expression of collagen type II and TGF-β1 were significantly higher in the ASCs than in the control group. In conclusion, ASCs have regenerative effects on the auricular cartilage defect of the rabbit. These effects would be expected to contribute significantly to the regeneration of damaged cartilage tissue in vivo.

Comparison of PLGA/Fibrin and PLGA/Hyaluronic Acid Scaffolds for Chondrogenesis of Human Adipose-Derived Stem Cells

2020 ◽  
Author(s):  
Zeinolabedin Sharifian ◽  
Batool Hashemibeni ◽  
Majid Pourentezari ◽  
Ali Valiani ◽  
Mohammad Mardani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Hyaluronic Acid ◽  
Real Time ◽  
Cartilage Regeneration ◽  
Type Ii Collagen ◽  
Control Group ◽  
Adipose Derived Stem Cells ◽  
X Gene

Background and Aims: Tissue engineering is a relatively novel field that has been intensely developing during recent years and has shown to be excessively promising when used for cartilage regeneration. Scaffolds represent important components for tissue engineering. Materials and Methods: The Poly Lactic-Co-Glycolic Acid (PLGA) impregnated with fibrin and hyaluronic acid (HA) produce hybrid scaffolds. human adipose-derived stem cells (hADSCs) were seeded in scaffolds and cultured in chondrogenic media. The viability of cells in different groups was assessed by MTT. The expression of chondrogenic related genes [Sox9, type II collagen (Col II), Aggrecan(AGG)] and type X collagen (Col X) was quantified by real-time polymerase chain reaction. Results: The results of the real-time PCR showed SOX9, AGG and Col X gene expression in the control groups being significantly lower than the other groups (p≤0.05). It also demonstrated Col II gene expression in the control group being significantly lower than the PLGA/Fibrin and PLGA/Fibrin/HA groups (p≤0.05). The Col X gene expression of cells in PLGA/HA and PLGA/Fibrin/HA groups significantly decreased in comparison with the PLGA/Fibrin group (p≤0.05). Conclusions: These conclusions indicate that administration of PLGA/ Fibrin and PLGA/HA scaffolds, particularly PLGA/Fibrin/ HA, motivates chondrogenesis in hADSCs. This can be diminished by decreasing hypertrophic markers and increasing characteristic markers of hyaline cartilage.

scholarly journals Electrospun Poly(ester-Urethane)- and Poly(ester-Urethane-Urea) Fleeces as Promising Tissue Engineering Scaffolds for Adipose-Derived Stem Cells

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e90676 ◽  
Author(s):  
Alfred Gugerell ◽  
Johanna Kober ◽  
Thorsten Laube ◽  
Torsten Walter ◽  
Sylvia Nürnberger ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Tissue Engineering Scaffolds

A Chondroitin Sulfate based Injectable Hydrogel for Delivery of Stem Cells in Cartilage Regeneration

2021 ◽  
Author(s):  
Xiaolin Li ◽  
Qian Xu ◽  
Melissa Johnson ◽  
Xi Wang ◽  
Jing Lyu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Chondroitin Sulfate ◽  
Cartilage Tissue Engineering ◽  
Cartilage Regeneration ◽  
Cartilage Tissue ◽  
Tissue Engineering Scaffolds ◽  
Injectable Hydrogel ◽  
Rapid Degradation

Chondroitin sulfate (CS), as a popular material for cartilage tissue engineering scaffolds, has been extensively studied and reported for its safety and excellent biocompatibility. However, the rapid degradation of pure...

scholarly journals Adipose-Derived Stem Cells for Tissue Engineering and Regenerative Medicine Applications

2016 ◽  
Vol 2016 ◽  
pp. 1-19 ◽  
Author(s):  
Ru Dai ◽  
Zongjie Wang ◽  
Roya Samanipour ◽  
Kyo-in Koo ◽  
Keekyoung Kim
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Regenerative Medicine ◽  
Three Dimensional ◽  
Adipose Derived Stem Cells ◽  
Tissue Engineering Scaffolds ◽  
Stem Cell Source ◽  
Proliferation And Differentiation ◽  
Mesenchymal Stem Cell Source

Adipose-derived stem cells (ASCs) are a mesenchymal stem cell source with properties of self-renewal and multipotential differentiation. Compared to bone marrow-derived stem cells (BMSCs), ASCs can be derived from more sources and are harvested more easily. Three-dimensional (3D) tissue engineering scaffolds are better able to mimic thein vivocellular microenvironment, which benefits the localization, attachment, proliferation, and differentiation of ASCs. Therefore, tissue-engineered ASCs are recognized as an attractive substitute for tissue and organ transplantation. In this paper, we review the characteristics of ASCs, as well as the biomaterials and tissue engineering methods used to proliferate and differentiate ASCs in a 3D environment. Clinical applications of tissue-engineered ASCs are also discussed to reveal the potential and feasibility of using tissue-engineered ASCs in regenerative medicine.

In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts

2017 ◽  
Vol 68 (6) ◽  
pp. 1341-1344
Author(s):  
Grigore Berea ◽  
Gheorghe Gh. Balan ◽  
Vasile Sandru ◽  
Paul Dan Sirbu
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Endothelial Cells ◽  
Single Cell ◽  
Cell Line ◽  
Bone Repair ◽  
Umbilical Vein ◽  
Human Fibroblasts ◽  
Three Dimensional ◽  
Adipose Derived Stem Cells

Complex interactions between stem cells, vascular cells and fibroblasts represent the substrate of building microenvironment-embedded 3D structures that can be grafted or added to bone substitute scaffolds in tissue engineering or clinical bone repair. Human Adipose-derived Stem Cells (hASCs), human umbilical vein endothelial cells (HUVECs) and normal dermal human fibroblasts (NDHF) can be mixed together in three dimensional scaffold free constructs and their behaviour will emphasize their potential use as seeding points in bone tissue engineering. Various combinations of the aforementioned cell lines were compared to single cell line culture in terms of size, viability and cell proliferation. At 5 weeks, viability dropped for single cell line spheroids while addition of NDHF to hASC maintained the viability at the same level at 5 weeks Fibroblasts addition to the 3D construct of stem cells and endothelial cells improves viability and reduces proliferation as a marker of cell differentiation toward osteogenic line.

Tendon Tissue Engineering: Mechanism and Effects of Human Tenocyte Coculture With Adipose-Derived Stem Cells

2018 ◽  
Vol 43 (2) ◽  
pp. 183.e1-183.e9 ◽  
Author(s):  
Chao Long ◽  
Zhen Wang ◽  
Anais Legrand ◽  
Arhana Chattopadhyay ◽  
James Chang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Tendon Tissue ◽  
Tendon Tissue Engineering

scholarly journals Epithelial differentiation of adipose-derived stem cells for laryngeal tissue engineering

2009 ◽  
Vol 120 (1) ◽  
pp. 125-131 ◽  
Author(s):  
Jennifer L. Long ◽  
Patricia Zuk ◽  
Gerald S. Berke ◽  
Dinesh K. Chhetri
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Epithelial Differentiation ◽  
Adipose Derived Stem Cells
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