A new colorimetric platform for ultrasensitive detection of protein and cancer cells based on the assembly of nucleic acids and proteins

2015 ◽  
Vol 880 ◽  
pp. 1-7 ◽  
Author(s):  
Chaohui Chen ◽  
Yufei Liu ◽  
Zhenhua Zheng ◽  
Guohua Zhou ◽  
Xinghu Ji ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Cancer Cells ◽  
Ultrasensitive Detection

627: Multitarget Gene Inhibition by Synthetic Nucleic Acids in Bladder Cancer Cells

2006 ◽  
Vol 175 (4S) ◽  
pp. 202-202 ◽  
Author(s):  
Yvonne Burmeister ◽  
Kai Kraemer ◽  
Susanne Fuessel ◽  
Matthias Kotzsch ◽  
Axel Meye ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Nucleic Acids ◽  
Cancer Cells ◽  
Bladder Cancer Cells ◽  
Gene Inhibition

Gene therapy promises accurate, targeted administration and overcoming drug resistance in diverse cancer cells

2021 ◽  
Author(s):  
Moataz Dowaidar
Keyword(s):  
Gene Therapy ◽  
Drug Resistance ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Clinical Research ◽  
Cancer Cells ◽  
Cationic Lipids ◽  
Target Cells ◽  
Genetic Components ◽  
Wide Range

Nucleic acid-based therapeutics such as siRNA and miRNA employ the silencing capabilities of the RNAi mechanism to affect the expression of one gene or several genes in target cells. Nucleic acid-based therapies enable accurate, targeted administration and overcoming drug resistance in diverse cancer cells. Several studies have shown that they can be utilized alongside pharmacological therapy to increase the efficacy of existing therapies. In addition, nucleic acid-based therapies have the potential to widen the spectrum of druggable targets for a range of diseases and emerge as a novel therapeutic technique for treating a number of diseases that are today untreatable. Nucleic acids are dependent on their effective distribution to target cells, which need correct complexation and encapsulation in a delivery mechanism. Although nucleic acids exist in a variety of forms and sizes, their physical and chemical commonality allow them to be loaded into a wide range of delivery vehicles. The primary biomaterials used to encapsulate genetic components were cationic lipids and polymers. Furthermore, the experiments focused particularly on effective transfection in target cells.Recent breakthroughs in NP-based RNA therapeutics have spurred a flood of clinical research, facing many challenges. In vivo, pharmacokinetics of different RNA-based medications must be researched to establish the viability and therapeutic potential of nucleic acid-based therapeutics. The U.S. Food and Drug Administration recently authorized many NP-based gene therapy. In 2019, Novartis authorized Zolgensma (onasemnogene abeparvovec-xioi) to treat spinal muscle atrophy. The first clinical research employing siRNA began in 2004 and is considered a milestone in nucleic acid-based drug development. Thirty clinical investigations have subsequently been completed. In 2018, the US FDA cleared Onpattro (Patisiran, Alnylam Pharmaceuticals) for the treatment of polyneuropathy caused by transthyretin amyloidosis.Several new generations of nucleic acid compositions employing polymer nanoparticles or liposomes are presently undergoing clinical testing. If allowed, the debut of nucleic acid-based treatments would represent a watershed event in immunotherapy. Advances in the design and development of biocompatible nanomaterials would allow us to overcome the above-mentioned problems and so show the potential to deliver nucleic acids in the treatment of a number of illnesses.

New Fluorescent Nanoparticles for Ultrasensitive Detection of Nucleic Acids by Optical Methods

ChemBioChem ◽  
2017 ◽  
Vol 18 (16) ◽  
pp. 1599-1603 ◽  
Author(s):  
Mads Westergaard Mulberg ◽  
Maria Taskova ◽  
Rasmus P. Thomsen ◽  
Anders H. Okholm ◽  
Jørgen Kjems ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Optical Methods ◽  
Fluorescent Nanoparticles ◽  
Ultrasensitive Detection

Triple-Helix Molecular Switch Electrochemical Ratiometric Biosensor for Ultrasensitive Detection of Nucleic Acids

2017 ◽  
Vol 89 (17) ◽  
pp. 8830-8835 ◽  
Author(s):  
Erhu Xiong ◽  
Zhenzhen Li ◽  
Xiaohua Zhang ◽  
Jiawan Zhou ◽  
Xiaoxia Yan ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Triple Helix ◽  
Molecular Switch ◽  
Ultrasensitive Detection

MULTITARGET GENE INHIBITION BY SYNTHETIC NUCLEIC ACIDS IN BLADDER CANCER CELLS

2006 ◽  
Vol 5 (2) ◽  
pp. 38
Author(s):  
Y. Burmeister ◽  
K. Kraemer ◽  
S. Fuessel ◽  
M. Kotzsch ◽  
A. Meye ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Nucleic Acids ◽  
Cancer Cells ◽  
Bladder Cancer Cells ◽  
Gene Inhibition
Sign in / Sign up

Export Citation Format

Share Document