Dual-Mode Ultrasensitive Detection of Nucleic Acids via an Aqueous “Seesaw” Strategy by Combining Aggregation-Induced Emission and Plasmonic Colorimetry

2018 ◽  
Vol 2 (1) ◽  
pp. 163-169 ◽  
Author(s):  
Jianlei Shen ◽  
Yiru Zhang ◽  
Rong Hu ◽  
Ryan T. K. Kwok ◽  
Zhiming Wang ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Ultrasensitive Detection ◽  
Dual Mode ◽  
Aggregation Induced Emission

scholarly journals Highly sensitive dual mode electrochemical platform for microRNA detection

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Pawan Jolly ◽  
Marina R. Batistuti ◽  
Anna Miodek ◽  
Pavel Zhurauski ◽  
Marcelo Mulato ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Dynamic Range ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Dual Mode ◽  
Electrode Surfaces ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Detection Mode ◽  
Amplification Strategy

Abstract MicroRNAs (miRNAs) play crucial regulatory roles in various human diseases including cancer, making them promising biomarkers. However, given the low levels of miRNAs present in blood, their use as cancer biomarkers requires the development of simple and effective analytical methods. Herein, we report the development of a highly sensitive dual mode electrochemical platform for the detection of microRNAs. The platform was developed using peptide nucleic acids as probes on gold electrode surfaces to capture target miRNAs. A simple amplification strategy using gold nanoparticles has been employed exploiting the inherent charges of the nucleic acids. Electrochemical impedance spectroscopy was used to monitor the changes in capacitance upon any binding event, without the need for any redox markers. By using thiolated ferrocene, a complementary detection mode on the same sensor was developed where the increasing peaks of ferrocene were recorded using square wave voltammetry with increasing miRNA concentration. This dual-mode approach allows detection of miRNA with a limit of detection of 0.37 fM and a wide dynamic range from 1 fM to 100 nM along with clear distinction from mismatched target miRNA sequences. The electrochemical platform developed can be easily expanded to other miRNA/DNA detection along with the development of microarray platforms.

Dual-mode fluorescent and colorimetric immunoassay for the ultrasensitive detection of alpha-fetoprotein in serum samples

2018 ◽  
Vol 1038 ◽  
pp. 112-119 ◽  
Author(s):  
Yaofeng Zhou ◽  
Xiaolin Huang ◽  
Sicheng Xiong ◽  
Xiangmin Li ◽  
Shengnan Zhan ◽  
...  
Keyword(s):  
Alpha Fetoprotein ◽  
Ultrasensitive Detection ◽  
Dual Mode ◽  
Serum Samples ◽  
Colorimetric Immunoassay

New Fluorescent Nanoparticles for Ultrasensitive Detection of Nucleic Acids by Optical Methods

ChemBioChem ◽  
2017 ◽  
Vol 18 (16) ◽  
pp. 1599-1603 ◽  
Author(s):  
Mads Westergaard Mulberg ◽  
Maria Taskova ◽  
Rasmus P. Thomsen ◽  
Anders H. Okholm ◽  
Jørgen Kjems ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Optical Methods ◽  
Fluorescent Nanoparticles ◽  
Ultrasensitive Detection

scholarly journals Ultrasensitive detection of aqueous Cu2+ ions by a coumarin-salicylidene based AIEgen

2019 ◽  
Vol 3 (11) ◽  
pp. 2437-2447 ◽  
Author(s):  
Subrata Kumar Padhan ◽  
Narayan Murmu ◽  
Subrat Mahapatra ◽  
M. K. Dalai ◽  
Satya Narayan Sahu
Keyword(s):  
Schiff Base ◽  
Ultrasensitive Detection ◽  
Aggregation Induced Emission ◽  
Nanomolar Range

This work reports the aggregation induced emission (AIE) behaviour of a coumarin-salicylidene Schiff base probe 3 which demonstrates ultrasensitive detection of aqueous Cu2+ ions in nanomolar range via a fluorescence “on–off” strategy.

Triple-Helix Molecular Switch Electrochemical Ratiometric Biosensor for Ultrasensitive Detection of Nucleic Acids

2017 ◽  
Vol 89 (17) ◽  
pp. 8830-8835 ◽  
Author(s):  
Erhu Xiong ◽  
Zhenzhen Li ◽  
Xiaohua Zhang ◽  
Jiawan Zhou ◽  
Xiaoxia Yan ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Triple Helix ◽  
Molecular Switch ◽  
Ultrasensitive Detection

Aggregation-induced emission active D-π-A binaphthyl luminophore with dual-mode fluorescence

RSC Advances ◽  
2014 ◽  
Vol 4 (63) ◽  
pp. 33474 ◽  
Author(s):  
Shunsuke Sasaki ◽  
Yosuke Niko ◽  
Kazunobu Igawa ◽  
Gen-ichi Konishi
Keyword(s):  
Dual Mode ◽  
Aggregation Induced Emission

scholarly journals Luminescent Probes for Ultrasensitive Detection of Nucleic Acids

2010 ◽  
Vol 21 (2) ◽  
pp. 319-327 ◽  
Author(s):  
Lev N. Krasnoperov ◽  
Salvatore A. E. Marras ◽  
Maxim Kozlov ◽  
Laura Wirpsza ◽  
Arkady Mustaev
Keyword(s):  
Nucleic Acids ◽  
Ultrasensitive Detection ◽  
Luminescent Probes
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