scholarly journals Multi-response optimization of the extraction and derivatization protocol of selected polar metabolites from apple fruit tissue for GC–MS analysis

2014 ◽  
Vol 824 ◽  
pp. 42-56 ◽  
Author(s):  
Elias A. Bekele ◽  
Carlo E.P. Annaratone ◽  
Maarten L.A.T.M. Hertog ◽  
Bart M. Nicolai ◽  
Annemie H. Geeraerd
Keyword(s):  
Apple Fruit ◽  
Fruit Tissue ◽  
Ms Analysis ◽  
Polar Metabolites ◽  
Response Optimization

Ion Relations of Apple Fruit Tissue During Fruit Development and Ripening. I. Cation Leakage

1981 ◽  
Vol 8 (2) ◽  
pp. 155 ◽  
Author(s):  
IB Ferguson ◽  
CB Watkins
Keyword(s):  
Fruit Development ◽  
Tissue Concentration ◽  
Apple Fruit ◽  
Divalent Ions ◽  
Fruit Tissue ◽  
Fruit Development And Ripening ◽  
Ion Relations ◽  
Calcium Magnesium ◽  
Calcium And Magnesium ◽  
Potassium Leakage

Leakage of calcium, magnesium and potassium from discs of cortical apple fruit tissue was followed through fruit development and ripening. Leakage of potassium always exceeded that of calcium and magnesium and was little affected by the external presence of the divalent ions. Calcium and magnesium leakage was markedly increased by the external presence of either ion. In tissue from both freshly picked fruit and that taken from storage, potassium and magnesium leakage increased when the fruit was in an advanced state of senescence, but calcium leakage decreased. During fruit development, leakage of all cations was closely related to availability as expressed in tissue concentration. There was a marked increase in potassium leakage in association with the respiratory climacteric.

THE FLUORESCENCE OF FROZEN POTATO TUBER AND APPLE FRUIT TISSUE UNDER ULTRA-VIOLET LIGHT

1945 ◽  
Vol 23c (2) ◽  
pp. 76-78 ◽  
Author(s):  
W. Newton ◽  
W. Jones
Keyword(s):  
Potato Tuber ◽  
Ultra Violet ◽  
Apple Fruit ◽  
Potato Tubers ◽  
Tuber Tissue ◽  
Fruit Tissue ◽  
Potato Tuber Tissue ◽  
Violet Light ◽  
Apple Tissue ◽  
Acetone Extracts

Freshly cut sections of frozen potato tuber and apple fruit tissue exhibit a brilliant fluorescence when examined in a dark room under an ultra-violet (Stroblite) lamp. The fluorescence disappears from the apple tissue upon thawing but is retained in potato tuber tissue. Thus the ultra-violet lamp serves as a useful diagnostic means of detecting frost or low temperature injury in potato tubers. Many other plant tissues were frozen and examined, but proved to be non-fluorescent. Acetone extracts of both normal and frozen potato tuber tissue were fluorescent, but similar extracts of both frozen and normal apple fruit were not. Although normal potato tissue is non-fluorescent and frozen tissue is brightly fluorescent, no differences were found in the brightness of the acetone extracts.

Ion Relations of Apple Fruit Tissue During Fruit Development and Ripening. II. Phosphate Uptake

1981 ◽  
Vol 8 (3) ◽  
pp. 249 ◽  
Author(s):  
CB Watkins ◽  
IB Ferguson
Keyword(s):  
Fruit Development ◽  
Phosphate Uptake ◽  
Apple Fruit ◽  
Cortical Tissue ◽  
Fresh Tissue ◽  
Fruit Tissue ◽  
Metabolic Component ◽  
Aging Response ◽  
Fruit Development And Ripening ◽  
Ion Relations

Phosphate uptake by discs of cortical tissue from apple fruit was followed during fruit development and ripening. Uptake from 10-3 M phosphate could be separated into a rapid, free-space uptake phase, followed by a plateau in the uptake rate, and then an increase in rate which was under metabolic control. During fruit development, the metabolic component of phosphate uptake underwent the greatest change. Apple fruit tissue exhibited an aging response in phosphate uptake. Accelerated uptake was found after 6 h aging and, after 20 h aging, the rate of phosphate uptake by the tissue was greatly enhanced. Aged tissue incorporated 32P into the ester fraction within 1 h, in contrast to fresh tissue where incorporation in 1 h was negligible.

Proteomic fingerprinting of apple fruit, juice, and cider via combinatorial peptide ligand libraries and MS analysis

2018 ◽  
Vol 40 (2) ◽  
pp. 266-271 ◽  
Author(s):  
María Jesús Lerma-García ◽  
Maria Nicoletti ◽  
Ernesto Francisco Simó-Alfonso ◽  
Pier Giorgio Righetti ◽  
Elisa Fasoli
Keyword(s):  
Fruit Juice ◽  
Apple Fruit ◽  
Peptide Ligand ◽  
Ms Analysis ◽  
Proteomic Fingerprinting

Soluble and wall-bound glycoproteins of apple fruit tissue

1975 ◽  
Vol 14 (10) ◽  
pp. 2181-2188 ◽  
Author(s):  
Michael Knee
Keyword(s):  
Apple Fruit ◽  
Fruit Tissue

scholarly journals Comparative Transcriptomic Analysis of the Interaction between Penicillium expansum and Apple Fruit (Malus pumila Mill.) during Early Stages of Infection

2019 ◽  
Vol 7 (11) ◽  
pp. 495 ◽  
Author(s):  
Kaili Wang ◽  
Xiangfeng Zheng ◽  
Xiaoyun Zhang ◽  
Lina Zhao ◽  
Qiya Yang ◽  
...  
Keyword(s):  
Early Stage ◽  
Ph Regulation ◽  
Apple Fruit ◽  
Infection Process ◽  
Penicillium Expansum ◽  
Postharvest Disease ◽  
Economic Losses ◽  
Post Inoculation ◽  
Fruit Tissue ◽  
Early Stages

Blue mold, caused by Penicillium expansum, is an important postharvest disease of apple, and can result in significant economic losses. The present study investigated the interaction between P. expansum and wounded apple fruit tissues during the early stages of the infection. Spores of P. expansum became activated one hour post-inoculation (hpi), exhibited swelling at 3 hpi, and the germ tubes were found entering into apple tissues at 6 hpi. RNA-seq was performed on samples of P. expansum and apple fruit tissue collected at 1, 3, and 6 hpi. The main differentially expressed genes (DEGs) that were identified in P. expansum were related to interaction, cell wall degradation enzymes, anti-oxidative stress, pH regulation, and effectors. Apple tissues responded to the presence of P. expansum by activating pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) at 1 hpi, then activated effector-triggered immunity (ETI) at 3 hpi. This research provides new information on the interaction between P. expansum and apple fruit tissue at an early stage of the infection process.

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