Method development and validation for the simultaneous determination of rosiglitazone and metformin in pharmaceutical preparations by capillary zone electrophoresis

2005 ◽  
Vol 549 (1-2) ◽  
pp. 88-95 ◽  
Author(s):  
Ceren Yardımcı ◽  
Nuran Özaltın
Keyword(s):  
Simultaneous Determination ◽  
Capillary Zone Electrophoresis ◽  
Method Development ◽  
Pharmaceutical Preparations ◽  
Zone Electrophoresis ◽  
Method Development And Validation ◽  
Capillary Zone ◽  
Development And Validation

scholarly journals Simultaneous Determination of Sitagliptin and Metformin in Pharmaceutical Preparations by Capillary Zone Electrophoresis and its Application to Human Plasma Analysis

2012 ◽  
Vol 7 ◽  
pp. ACI.S9940 ◽  
Author(s):  
Mohamed Salim ◽  
Nahed El-Enany ◽  
Fathallah Belal ◽  
Mohamed Walash ◽  
Gabor Patonay
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
Capillary Zone Electrophoresis ◽  
Pharmaceutical Preparations ◽  
Effective Length ◽  
Relative Standard ◽  
Significant Difference ◽  
Zone Electrophoresis ◽  
Capillary Zone

A novel, quick, reliable and simple capillary zone electrophoresis CZE method was developed and validated for the simultaneous determination of sitagliptin (SG) and metformin (MF) in pharmaceutical preparations. Separation was carried out in fused silica capillary (50.0 cm total length and 43.0 cm effective length, 49 μm i.d.) by applying a potential of 15 KV (positive polarity) and a running buffer containing 60 mM phosphate buffer at pH 4.0 with UV detection at 203 nm. The samples were injected hydrodynamically for 3 s at 0.5 psi and the temperature of the capillary cartridge was kept at 25 °C. Phenformin was used as internal standard (IS). The method was suitably validated with respect to specificity, linearity, limit of detection and quantitation, accuracy, precision, and robustness. The method showed good linearity in the ranges of 10-100 μg/mL and 50-500 μg/mL with limits of detection of 0.49, 2.11 μm/mL and limits of quantification of 1.48, 6.39 μg/mL for SG and MF, respectively. The proposed method was successfully applied for the analysis of the studied drugs in their synthetic mixtures and co-formulated tablets without interfering peaks due to the excipients present in the pharmaceutical tablets. The method was further extended to the in-vitro determination of the two drugs in spiked human plasma. The estimated amounts of SG/MF were almost identical with the certified values, and their percentage relative standard deviation values (% R.S.D.) were found to be ≤1.50% (n = 3). The results were compared to a reference method reported in the literature and no significant difference was found statistically.

Simultaneous determination of ofloxacin and ornidazole in pharmaceutical preparations by capillary zone electrophoresis

2009 ◽  
Vol 23 (12) ◽  
pp. 1283-1290 ◽  
Author(s):  
Khoo Lay See ◽  
Abdalla A. Elbashir ◽  
Bahruddin Saad ◽  
Abdussalam Salhin Mohamed Ali ◽  
Hassan Y. Aboul-Enein
Keyword(s):  
Simultaneous Determination ◽  
Capillary Zone Electrophoresis ◽  
Pharmaceutical Preparations ◽  
Zone Electrophoresis ◽  
Capillary Zone

scholarly journals Method Development and Validation for the Simultaneous Determination of Four Coumarins in Saussurea superba by Capillary Zone Electrophoresis

2010 ◽  
Vol 93 (5) ◽  
pp. 1410-1415 ◽  
Author(s):  
Juan Chen ◽  
Li Chen ◽  
Hong-Li Zhang ◽  
Yan-Ping Shi
Keyword(s):  
Simultaneous Determination ◽  
Applied Voltage ◽  
Method Development ◽  
Migration Behavior ◽  
Electrophoretic Method ◽  
Wide Range ◽  
Method Development And Validation ◽  
Capillary Zone ◽  
Development And Validation

Abstract A capillary zone electrophoretic method has been developed for the determination of four coumarinsskimmin, scopolin, scopoletin, and umbelliferonein Saussurea superba with UV detection at 254 nm. The capillary temperature was kept constant at 25°C. Effects of buffer pH, electrolyte concentration, organic modifier, and applied voltage on migration behavior were studied systematically. The optimum conditions for separation were achieved by using 30 mM borate buffer at pH 9.02 containing 15% (v/v) methanol as the electrolyte and 25 kV as the applied voltage. For all analytes a good linear regression relationship (r >0.999) was obtained between peak area and concentration over a relatively wide range. The method was validated for repeatability, precision, and accuracy. The validated method was successfully applied to the simultaneous determination of the four analytes in S. superba.

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