Imaging DNA single-strand breaks generated by reactive oxygen species using a liquid crystal-based sensor

2018 ◽  
Vol 556 ◽  
pp. 1-6 ◽  
Author(s):  
Hyeong Jin Kim ◽  
Chang-Hyun Jang
Keyword(s):  
Reactive Oxygen Species ◽  
Liquid Crystal ◽  
Reactive Oxygen ◽  
Single Strand ◽  
Oxygen Species ◽  
Strand Breaks ◽  
Single Strand Breaks

scholarly journals Interaction of Nitric Oxide with Glutathione or Cysteine Generates Reactive Oxygen Species Causing DNA Single Strand Breaks

2005 ◽  
Vol 28 (6) ◽  
pp. 998-1003 ◽  
Author(s):  
Kiyomi Kikugawa ◽  
Natsuki Oikawa ◽  
Azusa Miyazawa ◽  
Ken-ichi Shindo ◽  
Tetsuta Kato
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Single Strand ◽  
Oxygen Species ◽  
Strand Breaks ◽  
Single Strand Breaks

Stannous chloride mediates single strand breaks in plasmid DNA through reactive oxygen species formation

1999 ◽  
Vol 110 (3) ◽  
pp. 129-136 ◽  
Author(s):  
Flávio J.S. Dantas ◽  
Milton O. Moraes ◽  
José C.P. de Mattos ◽  
Roberto J.A.C. Bezerra ◽  
Elizeu F. Carvalho ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Plasmid Dna ◽  
Stannous Chloride ◽  
Reactive Oxygen ◽  
Single Strand ◽  
Oxygen Species ◽  
Reactive Oxygen Species Formation ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Species Formation

scholarly journals Requirement for human AP endonuclease 1 for repair of 3′-blocking damage at DNA single-strand breaks induced by reactive oxygen species

2000 ◽  
Vol 21 (7) ◽  
pp. 1329-1334
Author(s):  
Tadahide Izumi ◽  
Tapas K. Hazra ◽  
Istvan Boldogh ◽  
Alan E. Tomkinson ◽  
Min S. Park ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Single Strand ◽  
Oxygen Species ◽  
Ap Endonuclease ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Ap Endonuclease 1

scholarly journals Requirement for human AP endonuclease 1 for repair of 3′-blocking damage at DNA single-strand breaks induced by reactive oxygen species

2000 ◽  
Vol 21 (7) ◽  
pp. 1329-1334 ◽  
Author(s):  
Tadahide Izumi ◽  
Tapas K. Hazra ◽  
Istvan Boldogh ◽  
Alan E. Tomkinson ◽  
Min S. Park ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Single Strand ◽  
Oxygen Species ◽  
Ap Endonuclease ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Ap Endonuclease 1

Stimulation of poly(ADP-ribose) synthesis by free radicals in C3H10T1/2 cells: relationship with NAD metabolism and DNA breakage

1990 ◽  
Vol 68 (3) ◽  
pp. 602-608 ◽  
Author(s):  
Dominique Lautier ◽  
Danièle Poirier ◽  
Annie Boudreau ◽  
Moulay A. Alaoui Jamali ◽  
André Castonguay ◽  
...  
Keyword(s):  
Free Radicals ◽  
Active Oxygen Species ◽  
Accurate Determination ◽  
Active Oxygen ◽  
Single Strand ◽  
Oxygen Species ◽  
Strand Breaks ◽  
Dna Breakage ◽  
Single Strand Breaks ◽  
Stimulation Of

We have studied the effect of H2O2 and O2− produced by xanthine and xanthine oxidase on NAD catabolism, poly(ADP-ribose) synthesis, and production of DNA single-strand breaks in C3H10T1/2 cells. The results show a correlation between the induction of DNA single-strand breaks, the decrease of NAD pool, and the accumulation of polymer. New techniques, based on affinity chromatography and reversed-phase high pressure liquid chromatography, have allowed an accurate determination of polymer contents and showed a 20-fold stimulation of polymer biosynthesis induced by active oxygen species. Inhibition experiments performed with 3-aminobenzamide have shown that the decrease in NAD levels after exposure of cells to active oxygen species was caused by stimulation of poly(ADP-ribosyl)ation and of another cellular process.Key words: poly(ADP-ribose), free radicals, C3H10T1/2 cells, DNA breakage, NAD catabolism.

scholarly journals Through Its Nonstructural Protein NS1, Parvovirus H-1 Induces Apoptosis via Accumulation of Reactive Oxygen Species

2010 ◽  
Vol 84 (12) ◽  
pp. 5909-5922 ◽  
Author(s):  
Georgi Hristov ◽  
Melanie Krämer ◽  
Junwei Li ◽  
Nazim El-Andaloussi ◽  
Rodrigo Mora ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Nonstructural Protein ◽  
Reactive Oxygen ◽  
Double Strand Breaks ◽  
Oxygen Species ◽  
Ns1 Protein ◽  
Dna Double Strand Breaks ◽  
Caspase 9 ◽  
Strand Breaks ◽  
Cycle Arrest

ABSTRACT The rat parvovirus H-1 (H-1PV) attracts high attention as an anticancer agent, because it is not pathogenic for humans and has oncotropic and oncosuppressive properties. The viral nonstructural NS1 protein is thought to mediate H-1PV cytotoxicity, but its exact contribution to this process remains undefined. In this study, we analyzed the effects of the H-1PV NS1 protein on human cell proliferation and cell viability. We show that NS1 expression is sufficient to induce the accumulation of cells in G2 phase, apoptosis via caspase 9 and 3 activation, and cell lysis. Similarly, cells infected with wild-type H-1PV arrest in G2 phase and undergo apoptosis. Furthermore, we also show that both expression of NS1 and H-1PV infection lead to higher levels of intracellular reactive oxygen species (ROS), associated with DNA double-strand breaks. Antioxidant treatment reduces ROS levels and strongly decreases NS1- and virus-induced DNA damage, cell cycle arrest, and apoptosis, indicating that NS1-induced ROS are important mediators of H-1PV cytotoxicity.

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