Gold nanoparticle-based colorimetric detection of kanamycin using a DNA aptamer

2011 ◽  
Vol 415 (2) ◽  
pp. 175-181 ◽  
Author(s):  
Kyung-Mi Song ◽  
Minseon Cho ◽  
Hunho Jo ◽  
Kyoungin Min ◽  
Sung Ho Jeon ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Colorimetric Detection ◽  
Dna Aptamer

scholarly journals Optimisation of a gold nanoparticle-based aptasensor integrated with image processing for the colorimetric detection of acephate using response surface methodology

RSC Advances ◽  
2021 ◽  
Vol 11 (42) ◽  
pp. 25933-25942
Author(s):  
Mohd Junaedy Osman ◽  
Jahwarhar Izuan Abdul Rashid ◽  
Ong Keat Khim ◽  
Wan Md Zin Wan Yunus ◽  
Siti Aminah Mohd Noor ◽  
...  
Keyword(s):  
Image Processing ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Gold Nanoparticle ◽  
Magnesium Sulphate ◽  
Colorimetric Detection ◽  
Processing Technique ◽  
Dna Aptamer ◽  
Image Processing Technique ◽  
Modified Dna

We successfully optimized AuNPs, modified DNA aptamer and magnesium sulphate salt to enhance the selectivity and sensitivity for detection of Ac. The accuracy of the detection was also improved by image processing technique.

Colorimetric Detection with Aptamer-Gold Nanoparticle Conjugates: Effect of Aptamer Length on Response

2012 ◽  
Author(s):  
Jorge L. Chavez ◽  
Nancy Kelley-Loughnane ◽  
Morley O. Stone ◽  
Robert I. MacCuspie
Keyword(s):  
Gold Nanoparticle ◽  
Colorimetric Detection

scholarly journals CRISPR/Cas12a-mediated gold nanoparticle aggregation for colorimetric detection of SARS-CoV-2

2021 ◽  
Author(s):  
Yiren Cao ◽  
Jinjun Wu ◽  
Bo Pang ◽  
Hongquan Zhang ◽  
X. Chris Le
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Colorimetric Detection ◽  
Isothermal Amplification ◽  
Nanoparticle Aggregation ◽  
Cleavage Activity ◽  
Gold Nanoparticle Aggregation

The trans-cleavage activity of the target-activated CRISPR-Cas12a liberated an RNA crosslinker from a molecular transducer, which facilitated assembly of gold nanoparticles. Integration of the molecular transducer with isothermal amplification and...

scholarly journals Femtomolar and selective dopamine detection by a gold nanoparticle enhanced surface plasmon resonance aptasensor

2018 ◽  
Author(s):  
Yong Cao ◽  
Mark T. McDermott
Keyword(s):  
Surface Plasmon Resonance ◽  
Detection Limit ◽  
Gold Nanoparticle ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Cdna Probe ◽  
Dna Aptamer ◽  
Dopamine Detection ◽  
Calibration Curves ◽  
20 Nm

ABSTRACTUltrasensitive and selective detection and quantification of dopamine (DA) plays a key role in monitoring neurodegenerative diseases. However, the detection limit reported for DA detection is typically in the lower nM range. Pushing the detection limit to pM or lower for this particular target to cover the physiological levels (< 130 pM) is significant. Herein, DA DNA aptamer (DAAPT) gold nanoparticle (AuNP) conjugate is utilized to enhance the surface plasmon resonance (SPR) signal, which enables to detect and quantify DA in the femtomolar (200 fM) to picomolar range. To the best of our knowledge, this is the lowest detection limit achieved for SPR sensing of dopamine. The as-prepared 10 nm DAAPT-AuNP conjugate demonstrates strong binding affinity (Kd = 3.1 ± 1.4 nM) to the complementary DNA (cDNA) probe on gold chip. The cDNA probe is immobilized to the chip via polydopamine surface chemistry, which allows the Michael addition of any primary amine-terminated biomolecules. By adjusting the concentration of the DAAPT-AuNP conjugate, two calibration curves are generated with dynamic ranges from 100 µM to 2 mM, and from 200 fM to 20 nM, respectively. Both calibration curves have negative slopes, showing good agreement to a dose-response curve in an enzyme inhibition assay. In addition, the sensing strategy is evaluated to be specific for DA detection using a series of DA analogs and other metabolites as potential interferences.

scholarly journals A portable lab-on-a-chip system for gold-nanoparticle-based colorimetric detection of metal ions in water

2014 ◽  
Vol 8 (5) ◽  
pp. 052107 ◽  
Author(s):  
Chen Zhao ◽  
Guowei Zhong ◽  
Da-Eun Kim ◽  
Jinxia Liu ◽  
Xinyu Liu
Keyword(s):  
Metal Ions ◽  
Gold Nanoparticle ◽  
Colorimetric Detection ◽  
Lab On A Chip

Gold nanoparticle-DNA aptamer-assisted delivery of antimicrobial peptide effectively inhibits Acinetobacter baumannii infection in mice

2021 ◽  
Vol 60 (1) ◽  
pp. 128-136
Author(s):  
Jaeyeong Park ◽  
Eunkyoung Shin ◽  
Ji-Hyun Yeom ◽  
Younkyung Choi ◽  
Minju Joo ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Acinetobacter Baumannii ◽  
Gold Nanoparticle ◽  
Dna Aptamer ◽  
Assisted Delivery

Ultrasensitive Colorimetric Detection of 17β-Estradiol: The Effect of Shortening DNA Aptamer Sequences

2015 ◽  
Vol 87 (8) ◽  
pp. 4201-4209 ◽  
Author(s):  
Omar A. Alsager ◽  
Shalen Kumar ◽  
Bicheng Zhu ◽  
Jadranka Travas-Sejdic ◽  
Kenneth P. McNatty ◽  
...  
Keyword(s):  
Colorimetric Detection ◽  
Dna Aptamer ◽  
17Β Estradiol

A sensitive gold nanoparticle-based aptasensor for colorimetric detection of Aβ1–40 oligomers

2018 ◽  
Vol 10 (6) ◽  
pp. 641-645 ◽  
Author(s):  
Xu Zhu ◽  
Ningning Zhang ◽  
Yintang Zhang ◽  
Baoxia Liu ◽  
Zhu Chang ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Colorimetric Detection ◽  
Label Free ◽  
Homogeneous Phase

A gold nanoparticle-based label-free homogeneous phase colorimetric bioassay was developed for the detection of Aβ1–40 oligomers.

scholarly journals A gold nanoparticle-intercalated mesoporous silica-based nanozyme for the selective colorimetric detection of dopamine

2020 ◽  
Vol 2 (2) ◽  
pp. 734-745 ◽  
Author(s):  
Shounak Ray ◽  
Rima Biswas ◽  
Rumeli Banerjee ◽  
Papu Biswas
Keyword(s):  
Mesoporous Silica ◽  
Gold Nanoparticle ◽  
Colorimetric Detection

A AuNP nanoparticle-incorporated mesoporous silica-based nanozyme exhibited the peroxidase-like activity and was utilized for the selective colorimetric detection of dopamine.

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