Silibinin ameliorates oxidative stress induced aberrant crypt foci and lipid peroxidation in 1, 2 dimethylhydrazine induced rat colon cancer

2009 ◽  
Vol 28 (3) ◽  
pp. 225-233 ◽  
Author(s):  
Nagarajan Sangeetha ◽  
Selvaraj Aranganathan ◽  
Namasivayam Nalini
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Colon Cancer ◽  
Aberrant Crypt Foci ◽  
Rat Colon

Dietary folate protects against azoxymethane-induced aberrant crypt foci development and oxidative stress in rat colon

2011 ◽  
Vol 236 (9) ◽  
pp. 1005-1011 ◽  
Author(s):  
Khalid S Al-Numair ◽  
Mostafa I Waly ◽  
Amanat Ali ◽  
Mohamed M Essa ◽  
Mohamed F Farhat ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Aberrant Crypt Foci ◽  
Rat Colon ◽  
Dietary Folate ◽  
And Oxidative Stress

Halofuginone may suppresses azoxymethane-induced serum tumor necrosis factor-a synthesis and aberrant crypt foci progression in rat colon

2017 ◽  
Author(s):  
Orhan Corum ◽  
Ozgur Ozdemir ◽  
Enver Yazar
Keyword(s):  
Colon Cancer ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Thiobarbituric Acid ◽  
Aberrant Crypt Foci ◽  
Rat Colon ◽  
Albino Rats ◽  
Cancer Group ◽  
Blood Cytokines ◽  
Necrosis Factor

The aim of this research was to investigate the effect of halofuginone on the progression of azoxymethane-induced colon cancer in rats. A total of 38 male Wistar albino rats were divided into 4 groups: Control (n=8), Halofuginone (n=10, 0.4 mg/kg, PO, SID), Cancer (n=10, azoxymethane, 15 mg/kg, IP, once a week for two weeks) and Cancer + Halofuginone (n=10). After 18 weeks, blood samples were taken under anesthesia and all animals were sacrificed. Aberrant crypt foci in the colon were stained with methylene blue. Blood cytokines, thiobarbituric acid reactive substances, 13,14-dihydro-15-keto-prostaglandin F2a levels, hemogram and biochemical values were measured. The tumor necrosis factor-a level in the Cancer group was higher (P less than 0.05) than in other groups, while higher numbers of aberrant crypt foci were found in the Cancer group compared with the Cancer + Halofuginone group (P less than 0.05). In summary, it may be stated that halofuginone may warrant evaluation as a supportive drug in the treatment of colon cancer in the future.

scholarly journals Red Wheat Alters Colon Cancer Risk, Oxidative Stress, and the Gut Microbiome

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 1156-1156
Author(s):  
Chelsey Fiecke ◽  
Daniel Gallaher ◽  
Senay Simsek ◽  
Ashok Sharma
Keyword(s):  
Oxidative Stress ◽  
Colon Cancer ◽  
Gut Microbiome ◽  
Beta Diversity ◽  
Precancerous Lesions ◽  
Aberrant Crypt Foci ◽  
Aleurone Layer ◽  
Adaptation Period ◽  
Colon Tissue ◽  
Alpha And Beta Diversity

Abstract Objectives Red wheat, the class of wheat used to make yeast bread products, is associated with reductions in colon cancer biomarkers, regardless of refinement state. We hypothesized that red wheat as well as the phenol-rich aleurone and testa layers of red wheat would reduce colonic precancerous lesions and oxidative stress, and beneficially modulate the gut microbiome in rats with diet-induced obesity. Methods Rats were divided into seven groups (12/group) and fed a normal fat diet (NFD), high-fat diet (50% of total kcal as fat, HFD), whole red wheat + HFD (whR + HFD), refined red wheat + HFD (refR + HFD), refined white wheat + HFD (refW + HFD), aleurone layer + HFD (AL + HFD), or testa layer + HFD (TL + HFD). After a 14-day adaptation period, rats received two i.p. injections of the colon-specific carcinogen 1,2-dimethylhydrazine (DMH), administered one week apart. Sixty-three days after the second injection, colons were harvested and precancerous lesions (aberrant crypt foci, ACF) were enumerated. Staining intensity of 3-nitrotyrosine (3-NT) was determined immunohistochemically in distal colon tissue. Microbial DNA from cecal contents was sequenced using a 16S rRNA metagenomic approach. Differences in alpha and beta diversity, and microbial abundances were determined. Results Compared to the NFD, the HFD had a greater number of ACF, regardless of size (i.e., AC/ACF). The refR + HFD had significant reductions in medium ACF (3–5 AC/ACF; 2.62 vs. 4.28), large ACF (≥6 AC/ACF; 0.06 vs. 0.45), ACF multiplicity (1.58 vs. 2.01) and 3-NT (% positivity per ACF; 2.06% vs. 4.51%) compared to the HFD. All diets containing wheat reduced large ACF number. The TL + HFD and AL + HFD demonstrated trends for reducing ACF with 8 AC (0.06 vs. 0.18) and 3-NT (2.22% vs. 4.51%), respectively, compared to the HFD. Beta diversity significantly differed between diet groups (R2 = 0.27, P = 0.001), and there was greater abundance of Faecalitalea, Fusicatenibacter, and Lactobacillus in the cecal contents of rats fed wheat-containing diets. Conclusions Red wheat reduces precancerous lesions, oxidative stress, and beneficially modulates the gut microbiome relative to a non-wheat diet. The phenol-rich testa and aleurone layers alone had little influence on these outcomes. Funding Sources NDSU Collaborative Seed Grant Program.

Promising Chemoprevention of Colonic Aberrant Crypt Foci by Portunus segnis Muscle and Shell Extracts in Azoxymethane-Induced Colorectal Cancer in Rats

2020 ◽  
Vol 20 (17) ◽  
pp. 2041-2052
Author(s):  
Zahra Sahebi ◽  
Mozhgan Emtyazjoo ◽  
Pargol G. Mostafavi ◽  
Shahin Bonakdar
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Aberrant Crypt Foci ◽  
Male Rats ◽  
Rat Colon ◽  
Control Group ◽  
Therapeutic Effects ◽  
Portunus Segnis

Background and Purpose: This study subjected a rat model to the extracts of muscle and shell tissues from Portunus segnis to assess their therapeutic effects on the HT-29 colon cancer cells as well as on colonic Aberrant Crypt Foci (ACF) induced by Azoxymethane (AOM). Methods: The cell line was exposed to the extracts to compare the cytotoxicity of hexane, butanol, ethyl acetate, and water extract of muscle and ethanolic extract of the shell. Male rats (n=40) were assigned into control, positive, negative, and treatment groups. The animals were injected with AOM, except the control group, and then exposed to 250 and 500mg/kg of the crude extracts. Immunohistochemical localization of Bax and Bcl-2, as well as ACF and antioxidant enzymes, were evaluated in the rat colon. Results: The butanolic muscle extract and ethanolic shell one demonstrated an IC50 of 9.02±0.19μg/ml and 20.23±0.27μg/ml towards the cell line, respectively. Dietary exposure inhibited the ACF formation and crypt multiplicity in the colon compared to the cancer control group. The activity of SOD and CAT increased, while that of MDA decreased. The expression of Bax and Bcl-2 increased and decreased, respectively. Conclusion: Taken together, the results show that both extractions were suggested to be suppressive to AOMinduced colon cancer.

scholarly journals Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis

2013 ◽  
Vol 37 (4) ◽  
pp. 343-349 ◽  
Author(s):  
Vinícius Paula Venâncio ◽  
Eric Batista Ferreira ◽  
Maísa Ribeiro Pereira Lima Brigagão ◽  
Fernanda Borges de Araújo Paula ◽  
Luis Fernando Barbisan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cellular Localization ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Colon Carcinogenesis ◽  
Aberrant Crypt Foci ◽  
Nuclear Antigen ◽  
Rat Colon ◽  
Commercial Diet

A. crassiflora Mart. a Brazilian savannah fruit, is a source of phytochemical compounds that possess a wide array of biological activities, including free radical scavenging. This native fruit proved to potentialize the mutagenic process in previous in vivo investigations. The aim of the present study was to investigate the effects of A. crassiflora Mart. pulp intake on colonic cell proliferation and on the development of Aberrant Crypt Foci (ACF) in male Wistar rats. The animals were fed with either a commercial diet or a diet supplemented with A. crassiflora Mart. pulp mixed in 1%, 10% or 20% (w/w) for 4 weeks or 20 weeks. The carcinogen 1,2-dimethylhydrazine dihydrochloride (4 doses, 40 mg kg-1 each) was used to induce colonic ACF. After euthanasia, the blood, liver and colon samples were collected for biochemical determinations, oxidative stress or ACF development analysis, respectively. Immunohistochemical analyses of the colonic mucosa were performed using antibodies against proliferating cell nuclear antigen (PCNA) in normal-appearing colonic crypt and β-catenin in ACF. There was no ACF development in the colon from groups treated with A. crassiflora Mart. pulp. Also, the biochemical and oxidative stress analysis, PCNA labeling and ACF development (number, multiplicity or cellular localization of β-catenin) were unchanged as a result of marolo pulp intake. Thus, the present results suggest that A. crassiflora Mart. pulp intake did not exert any protective effect in the colon carcinogenesis induced by DMH in rats.

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