Investigating the Protective Effects of Astragalus Membranaceus on Nephrotoxicity in Cyclosporine A-treated Rats

Kidney ◽  
2010 ◽  
Vol 19 (3) ◽  
pp. 119-125 ◽  
Author(s):  
Ayman El-Meghawry El-Kenawy
Keyword(s):  
Cyclosporine A ◽  
Astragalus Membranaceus ◽  
Protective Effects

scholarly journals Protective Effects of HBSP on Ischemia Reperfusion and Cyclosporine A Induced Renal Injury

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Yuanyuan Wu ◽  
Junlin Zhang ◽  
Feng Liu ◽  
Cheng Yang ◽  
Yufang Zhang ◽  
...  
Keyword(s):  
Cyclosporine A ◽  
Caspase 3 ◽  
Interstitial Fibrosis ◽  
Ischemia Reperfusion ◽  
Inflammatory Cells ◽  
Protective Effects ◽  
Tubulointerstitial Damage ◽  
Apoptosis And Inflammation ◽  
The Right ◽  
Active Caspase

Ischemia reperfusion (IR) and cyclosporine A (CsA) injuries are unavoidable in kidney transplantation and are associated with allograft dysfunction. Herein, the effect and mechanism of a novel tissue protective peptide, helix B surface peptide (HBSP) derived from erythropoietin, were investigated in a rat model. The right kidney was subjected to 45 min ischemia, followed by left nephrectomy and 2-week reperfusion, with or without daily treatment of CsA 25 mg/kg and/or HBSP 8 nmol/kg. Blood urea nitrogen was increased by CsA but decreased by HBSP at 1 week and 2 weeks, while the same changes were revealed in urinary protein/creatinine only at 2 weeks. HBSP also significantly ameliorated tubulointerstitial damage and interstitial fibrosis, which were gradually increased by IR and CsA. In addition, apoptotic cells, infiltrated inflammatory cells, and active caspase-3+ cells were greatly reduced by HBSP in the both IR and IR + CsA groups. The 17 kD active caspase-3 protein was decreased by HBSP in the IR and IR + CsA kidneys, with decreased mRNA only in the IR + CsA kidneys. Taken together, it has been demonstrated, for the first time, that HBSP effectively improved renal function and tissue damage caused by IR and/or CsA, which might be through reducing caspase-3 activation and synthesis, apoptosis, and inflammation.

scholarly journals Switching From Tacrolimus to Cyclosporine A to Prevent Primary Biliary Cirrhosis Recurrence After Living-Donor Liver Transplantation

10.9738/cc188 ◽  
2013 ◽  
Vol 98 (2) ◽  
pp. 156-159 ◽  
Author(s):  
Hiroaki Shiba ◽  
Shigeki Wakiyama ◽  
Yasuro Futagawa ◽  
Takeshi Gocho ◽  
Ryusuke Ito ◽  
...  
Keyword(s):  
Liver Transplantation ◽  
Primary Biliary Cirrhosis ◽  
Cyclosporine A ◽  
Living Donor ◽  
Living Donor Liver Transplantation ◽  
Immunosuppressive Agents ◽  
Biliary Cirrhosis ◽  
Protective Effects ◽  
Donor Liver ◽  
Donor Liver Transplantation

Abstract Recurrence of primary biliary cirrhosis (PBC) after liver transplantation has been shown to negatively affect graft and patient survival. Recently, protective effects of cyclosporine A against PBC recurrence after liver transplantation have been reported. Participants were 4 patients who underwent living-donor liver transplantation (LDLT) for end-stage liver disease due to PBC. Tacrolimus was used for initial immunosuppression, and this was switched to cyclosporine A at least 3 months after liver transplantation. Targeted trough level of cyclosporine A was 20 times that of tacrolimus. We assessed liver and renal function, as well as antimitochondrial M2 antibody for recipients prior to LDLT, as well as before and after switching immunosuppressive agents. Patients were 1 man and 3 women, and they were ages 45 to 47 years at LDLT. Timing of switching from tacrolimus to cyclosporine A was 13, 3, 7, and 4 months respectively after liver transplantation, and all 4 patients have been on cyclosporine A without adverse effects at 20 to 46 months after transplantation. In 2 of 4 patients who had high titers of antimitochondrial M2 antibody before transplantation, antibody titer did not elevate after LDLT. In the other 2 patients without elevation of antimitochondrial M2 antibody, the titer did not turn positive. Switching from tacrolimus to cyclosporine A was possible without medical problems, and all patients exhibit no recurrence of PBC. Cyclosporine A may be useful for prevention of PBC recurrence after LDLT.

scholarly journals Vascular protective effects of Astragalus membranaceus and its main constituents in rats with chronic hyperhomocysteinemia

2017 ◽  
Vol 14 (3) ◽  
pp. 2401-2407 ◽  
Author(s):  
Li-Hong Qiu ◽  
Bi-Qi Zhang ◽  
Miao-Jun Lian ◽  
Xian-Ji Xie ◽  
Peng Chen
Keyword(s):  
Astragalus Membranaceus ◽  
Protective Effects

scholarly journals Astragalus membranaceus and Panax notoginseng, the Novel Renoprotective Compound, Synergistically Protect against Podocyte Injury in Streptozotocin-Induced Diabetic Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Ruonan Zhai ◽  
Guihua Jian ◽  
Teng Chen ◽  
Ling Xie ◽  
Rui Xue ◽  
...  
Keyword(s):  
Panax Notoginseng ◽  
Diabetic Rats ◽  
Astragalus Membranaceus ◽  
Protective Effects ◽  
The Novel ◽  
Podocyte Injury ◽  
Renal Histopathology ◽  
Foot Process ◽  
Immunofluorescence Labeling ◽  
Renal Expression

This study was aimed at investigating the synergistical protective effects of Astragalus membranaceus (AG) and Panax notoginseng (NG) on podocyte injury in diabetic rats. Diabetes was induced in rats by a single intraperitoneal injection of streptozotocin at 55 mg/kg. Diabetic rats were then orally administrated with losartan, AG, NG, and AG plus NG (2 : 1) for 12 weeks. Albuminuria, biochemical markers, renal histopathology, and podocyte number per glomerulus were measured. Podocyte apoptosis was determined by triple immunofluorescence labeling including TUNEL assay, WT1, and DAPI. Renal expression of nephrin, α-dystroglycan, Bax, Bcl-xl, and Nox4 was evaluated by immunohistochemistry, western blot, and RT-PCR. AG plus NG ameliorated albuminuria, renal histopathology, and podocyte foot process effacement to a greater degree than did AG or NG alone. The number of podocytes per glomerulus, as well as renal expression of nephrin, α-dystroglycan, and Bcl-xl, was decreased, while podocyte apoptosis, as well as renal expression of Bax and Nox4, was increased in diabetic rats. All of these abnormalities were partially restored by AG plus NG to a greater degree than did AG or NG alone. In conclusion, AG and NG synergistically ameliorated diabetic podocyte injury partly through upregulation of nephrin, α-dystroglycan, and Bcl-xl, as well as downregulation of Bax and Nox4. These findings might provide a novel treatment combination for DN.

scholarly journals Chemical Profiling of Astragalus membranaceus Roots (Fish.) Bunge Herbal Preparation and Evaluation of Its Bioactivity

2020 ◽  
Vol 15 (5) ◽  
pp. 1934578X2092415 ◽  
Author(s):  
Valentina Santoro ◽  
Valentina Parisi ◽  
Massimiliano D’Ambola ◽  
Chiara Sinisgalli ◽  
Magnus Monné ◽  
...  
Keyword(s):  
Respiratory Infections ◽  
Antioxidant Activities ◽  
Positive Control ◽  
Astragalus Membranaceus ◽  
Perennial Herb ◽  
Cholinesterase Inhibition ◽  
Protective Agent ◽  
Protective Effects ◽  
Simple Method ◽  
Free Hydroxy Group

Astragalus membranaceus (Fish.) Bunge is a perennial herb distributed in the northern part of China, and its roots, namely, Hang qi, are included as a natural ingredient in dietary supplement formulations commonly used to treat different disorders such as respiratory infections, diabetes, and heart failure. The availability of a simple method for the determination of the quality of Astragalus herbal preparations could be a challenging issue for commercial purposes. In this study, a liquid chromatography–mass spectrometry (LC–MS)/MS based approach was used to characterize specialized metabolite recovery of 3 commercial hydroalcoholic extracts of A. membranaceus (AMG1, AMG2, AMG3) in addition to a hydroalcoholic extract of A. membranaceus root (AST). The hypoglycemic effect, cholinesterase inhibition, and antioxidant activities were also evaluated. Thirty-one compounds, of which 19 polyphenols and 12 saponins, were identified. The extracts were also quantified by using a sensitive and selective Q-Trap system for their content in flavonoids and astragalosides, selecting astragaloside I and IV as chemical markers. From our results, AMG3 preparation (Axtragyl) was the most abundant in terms of both specialized classes of metabolites, showing a fingerprint similar to that of AST. Interestingly, tested enzyme inhibition ability of flavonoids, daidzein (11) and formononetin (19), reported a higher α-glucosidase inhibition in comparison with that of acarbose used as positive control. The in silico study clarified the interactions among the molecules and the importance of having a free hydroxy group. Moreover, Axtragyl was able to exert protective effects in Caco-2 cells treated with hydrogen peroxide, confirming its ability as a potential protective agent in intestinal injury.

Protective effects of a mixed plant extracts derived from Astragalus membranaceus and Laminaria japonica on PTU‐induced hypothyroidism and liver damages

2019 ◽  
Vol 43 (7) ◽  
Author(s):  
Md. Mohibbullah ◽  
Khawaja Muhammad Imran Bashir ◽  
Sung‐Kew Kim ◽  
Yong‐Ki Hong ◽  
Andre Kim ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Laminaria Japonica ◽  
Astragalus Membranaceus ◽  
Protective Effects

Protective Effects of Heme-Oxygenase Expression in Cyclosporine A - Induced Injury

2005 ◽  
Vol 2 (2) ◽  
pp. 157-161 ◽  
Author(s):  
Rita Rezzani ◽  
Luigi Rodella ◽  
Rossella Bianchi ◽  
Alvin Goodman ◽  
Elias Lianos
Keyword(s):  
Heme Oxygenase ◽  
Cyclosporine A ◽  
Protective Effects
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