scholarly journals A large open reading frame (orf1995 ) in the chloroplast DNA of Chlamydomonas reinhardtii encodes an essential protein

1997 ◽  
Vol 253 (5) ◽  
pp. 649-653 ◽  
Author(s):  
E. Boudreau ◽  
M. Turmel ◽  
M. Goldschmidt-Clermont ◽  
J.-D. Rochaix ◽  
S. Sivan ◽  
...  
Keyword(s):  
Chloroplast Dna ◽  
Chlamydomonas Reinhardtii ◽  
Open Reading Frame ◽  
Large Open Reading Frame ◽  
Reading Frame ◽  
Essential Protein

A photosystem II polypeptide is encoded by an open reading frame co-transcribed with genes for cytochrome b-559 in wheat chloroplast DNA

1989 ◽  
Vol 12 (2) ◽  
pp. 141-151 ◽  
Author(s):  
Andrew N. Webber ◽  
Sean M. Hird ◽  
Leonard C. Packman ◽  
Tristan A. Dyer ◽  
John C. Gray
Keyword(s):  
Photosystem Ii ◽  
Chloroplast Dna ◽  
Cytochrome B ◽  
Open Reading Frame ◽  
Reading Frame

Molecular Cloning and Characterization Analysis of Phosphofructokinase Gene from Dunaliella parva

2012 ◽  
Vol 518-523 ◽  
pp. 73-76
Author(s):  
Chang Hua Shang ◽  
Zhong Ming Wang ◽  
Shun Ni Zhu ◽  
Lei Qin ◽  
Zhen Hong Yuan
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Molecular Cloning ◽  
Sequence Similarity ◽  
Open Reading Frame ◽  
Volvox Carteri ◽  
Reading Frame ◽  
Untranslated Sequence ◽  
Oleaginous Microalgae ◽  
Dunaliella Parva ◽  
Fructose 1,6 Bisphosphate

Phosphofructokinase (PFK), which catalyses the phosphorylation of fructose-6-phosphate to fructose-1,6- bisphosphate, a key regulatory step in the glycolytic pathway. The former studies indicated the PFK could enhance glycolysis. The full-length cDNA encoding PFK was obtained from oleaginous microalgae Dunaliella parva, which include 1572 bp open reading frame (ORF), 254 bp 5′-untranslated sequence and 323 bp 3′-untranslated sequence. Dunaliella parva PFK showed the highest sequence similarity with the PFK from Chlamydomonas reinhardtii and Volvox carteri. The Dunaliella parva PFK also showed wide similarity with other species.

scholarly journals IRA1, an inhibitory regulator of the RAS-cyclic AMP pathway in Saccharomyces cerevisiae.

1989 ◽  
Vol 9 (2) ◽  
pp. 757-768 ◽  
Author(s):  
K Tanaka ◽  
K Matsumoto ◽  
A Toh-E
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cyclic Amp ◽  
Nitrogen Starvation ◽  
Open Reading Frame ◽  
Large Open Reading Frame ◽  
Ras Proteins ◽  
Base Pairs ◽  
Reading Frame ◽  
Phosphoprotein Phosphatase ◽  
Cdc25 Protein

A mutation in the gene IRA1 (formerly called PPD1) was originally characterized as a deficiency of a phosphoprotein phosphatase. The IRA1 gene has been cloned and sequenced. A large open reading frame (8,817 base pairs) which can encode a protein of 2,938 amino acids was found. Northern (RNA) blot analysis detected a message of about 10 kilobases, and nuclease S1 protection demonstrated mRNA start points at 97 and 98 base pairs upstream from the putative initiator ATG codon. Disruption of the IRA1 gene resulted in sensitivity to nitrogen starvation and heat shock. Diploids homozygous for the disrupted IRA1 gene were deficient in sporulation. Disruption of the IRA1 gene suppressed the lethality of the cdc25 mutation but did not suppress the lethality of either the ras1 ras2 or the cyr1 mutations. Deficiency of the phosphoprotein phosphatase was not reproducible in the disruption mutant of the IRA1 gene. Moreover, the ira1 mutant showed an increased level of cyclic AMP. Our results suggest that the IRA1 protein inhibits the function of the RAS proteins in a fashion antagonistic to the function of the CDC25 protein in the RAS-cyclic AMP pathway in Saccharomyces cerevisiae.

scholarly journals Sequence analysis and genomic organization of a new insect picorna-like virus, Ectropis obliqua picorna-like virus, isolated from Ectropis obliqua

2004 ◽  
Vol 85 (5) ◽  
pp. 1145-1151 ◽  
Author(s):  
Xiaochun Wang ◽  
Jiamin Zhang ◽  
Jie Lu ◽  
Fuming Yi ◽  
Chuanfeng Liu ◽  
...  
Keyword(s):  
Genome Organization ◽  
Genomic Organization ◽  
Sequence Similarity ◽  
Open Reading Frame ◽  
Large Open Reading Frame ◽  
Structural Genes ◽  
Rna Dependent Rna Polymerase ◽  
Sequence Comparisons ◽  
Reading Frame ◽  
Consensus Sequences

The complete nucleotide sequence of a new insect picorna-like virus, Ectropis obliqua picorna-like virus (EoPV), which causes a fatal infection of Ectropis obliqua larvae, has been determined. The genomic RNA of EoPV is 9394 nt in length and contains a single, large open reading frame (nt 391–9351) encoding a polyprotein of 2987 aa. Sequence comparisons with other viral polyproteins revealed that the consensus sequences for picornavirus RNA helicase, protease and RNA-dependent RNA polymerase proteins are found on the genome in order in the 5′→3′ direction. All structural genes were located at the 5′ terminus. In terms of sequence similarity, identity and genome organization, EoPV resembles mammalian picornaviruses and three other insect picorna-like viruses: Infectious flacherie virus of silkworm, Sacbrood virus of honeybee and Perina nuda picorna-like virus (PnPV). Phylogenetic analysis showed that EoPV is most closely related to PnPV and suggests that these four insect picorna-like viruses might constitute a new group of insect-infectious RNA viruses.

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