Euglena gracilis chloroplast DNA: analysis of a 1.6 kb intron of the psb C gene containing an open reading frame of 458 codons

1986 ◽  
Vol 11 (1) ◽  
pp. 35-39 ◽  
Author(s):  
Paul-Etienne Montandon ◽  
Alain Vasserot ◽  
Erhard Stutz
Keyword(s):  
Chloroplast Dna ◽  
Euglena Gracilis ◽  
Dna Analysis ◽  
Open Reading Frame ◽  
Reading Frame

A photosystem II polypeptide is encoded by an open reading frame co-transcribed with genes for cytochrome b-559 in wheat chloroplast DNA

1989 ◽  
Vol 12 (2) ◽  
pp. 141-151 ◽  
Author(s):  
Andrew N. Webber ◽  
Sean M. Hird ◽  
Leonard C. Packman ◽  
Tristan A. Dyer ◽  
John C. Gray
Keyword(s):  
Photosystem Ii ◽  
Chloroplast Dna ◽  
Cytochrome B ◽  
Open Reading Frame ◽  
Reading Frame

scholarly journals A large open reading frame (orf1995 ) in the chloroplast DNA of Chlamydomonas reinhardtii encodes an essential protein

1997 ◽  
Vol 253 (5) ◽  
pp. 649-653 ◽  
Author(s):  
E. Boudreau ◽  
M. Turmel ◽  
M. Goldschmidt-Clermont ◽  
J.-D. Rochaix ◽  
S. Sivan ◽  
...  
Keyword(s):  
Chloroplast Dna ◽  
Chlamydomonas Reinhardtii ◽  
Open Reading Frame ◽  
Large Open Reading Frame ◽  
Reading Frame ◽  
Essential Protein

scholarly journals The cDNA for rat selenoprotein P contains 10 TGA codons in the open reading frame

1991 ◽  
Vol 266 (16) ◽  
pp. 10050-10053
Author(s):  
K.E. Hill ◽  
R.S. Lloyd ◽  
J.G. Yang ◽  
R. Read ◽  
R.F. Burk
Keyword(s):  
Open Reading Frame ◽  
Selenoprotein P ◽  
Reading Frame

scholarly journals The Cloning and Molecular Analysis of pawn-B in Paramecium tetraurelia

Genetics ◽  
2000 ◽  
Vol 155 (3) ◽  
pp. 1105-1117 ◽  
Author(s):  
W John Haynes ◽  
Kit-Yin Ling ◽  
Robin R Preston ◽  
Yoshiro Saimi ◽  
Ching Kung
Keyword(s):  
Genomic Library ◽  
Open Reading Frame ◽  
Paramecium Tetraurelia ◽  
Wild Type ◽  
Rt Pcr ◽  
Reading Frame ◽  
Close Proximity ◽  
In The Wild ◽  
Dna Fragment ◽  
Alternative Sites

Abstract Pawn mutants of Paramecium tetraurelia lack a depolarization-activated Ca2+ current and do not swim backward. Using the method of microinjection and sorting a genomic library, we have cloned a DNA fragment that complements pawn-B (pwB/pwB). The minimal complementing fragment is a 798-bp open reading frame (ORF) that restores the Ca2+ current and the backward swimming when expressed. This ORF contains a 29-bp intron and is transcribed and translated. The translated product has two putative transmembrane domains but no clear matches in current databases. Mutations in the available pwB alleles were found within this ORF. The d4-95 and d4-96 alleles are single base substitutions, while d4-662 (previously pawn-D) harbors a 44-bp insertion that matches an internal eliminated sequence (IES) found in the wild-type germline DNA except for a single C-to-T transition. Northern hybridizations and RT-PCR indicate that d4-662 transcripts are rapidly degraded or not produced. A second 155-bp IES in the wild-type germline ORF excises at two alternative sites spanning three asparagine codons. The pwB ORF appears to be separated from a 5′ neighboring ORF by only 36 bp. The close proximity of the two ORFs and the location of the pwB protein as indicated by GFP-fusion constructs are discussed.

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