Enzyme histochemistry on human placental trophoblasts: the effect of fixation delay on enzyme activity

2000 ◽  
Vol 113 (4) ◽  
pp. 287-292 ◽  
Author(s):  
Shigeki Matsubara ◽  
Toshihiro Takizawa ◽  
Ikuo Sato
Keyword(s):  
Enzyme Activity ◽  
Enzyme Histochemistry ◽  
Fixation Delay

Parallel Intralysosomal Amyloid Fibrils, a Possible Result of Phagocytosis

1977 ◽  
Vol 14 (4) ◽  
pp. 407-419 ◽  
Author(s):  
E. Gruys ◽  
M. Castaño
Keyword(s):  
Electron Microscopy ◽  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Enzyme Histochemistry ◽  
Amyloid Fibrils ◽  
Light And Electron Microscopy ◽  
Interstitial Cells ◽  
Mesenchymal Cells ◽  
Parallel Arrangement

Vacuoles of mesenchymal cells in the papillae of bovine kidneys with amyloidosis were studied by histochemical electron microscopy for acid phosphatase as a marker for lysosomes. The vacuoles contained parallel amyloid fibrils. The vacuoles of reticular interstitial cells were found to be lysosomes. Vacuoles of macrophage-like cells of the same papillae were positive, partially positive, or negative for the enzyme activity. A suspension of papillary material was injected subcutaneously in rats in a 21-day light and electron microscopy and enzyme histochemistry study. Amyloid was demonstrated in vacuoles of macrophages throughout this period and initially also in neutrophils. In most vacuoles amyloid fibrils were randomly arranged but in others parallel arrangement was demonstrated. Amyloid was only at the inoculation sites. Intralysosomal bovine amyloid may occur in parallel fibrillar arrangement without a definite indication for amyloid production.

scholarly journals LOW TEMPERATURE EMBEDDING IN GLYCOL METHACRYLATE FOR ENZYME HISTOCHEMISTRY IN PLANT AND ANIMAL TISSUES

1972 ◽  
Vol 20 (12) ◽  
pp. 986-990 ◽  
Author(s):  
ANNE E. ASHFORD ◽  
WILLIAM G. ALLAWAY ◽  
MARGARET E. MCCULLY
Keyword(s):  
Enzyme Activity ◽  
Low Temperature ◽  
Enzyme Histochemistry ◽  
Animal Tissues ◽  
Glycol Methacrylate

A method is described by which enzyme activity can be retained in plant and animal tissues embedded in glycol methacrylate at low temperature.

Modulation of Glomerular ECTO-ADPase Expression by Oestradiol

1997 ◽  
Vol 77 (04) ◽  
pp. 767-771 ◽  
Author(s):  
M M Faas ◽  
W W Bakker ◽  
P A Klok ◽  
J F W Baller ◽  
G A Schuiling
Keyword(s):  
Enzyme Activity ◽  
Vessel Wall ◽  
Wistar Rats ◽  
Enzyme Histochemistry ◽  
Rat Kidney ◽  
Staining Intensity ◽  
Computerized Image Analysis ◽  
Ovx Rats ◽  
Dual Action ◽  
Plasma Factors

SummaryThe effect of 17-β-oestradiol (0E2) upon the activity of the glomerular anti-thrombotic ecto-enzyme ADPase was studied in cyclic and ovariectomized (OVX) Wistar rats. On day 0 (i.e. at the time of ovariectomy or 11 days after ovariectomy) ratsreceived OE2-releasing Silastic implants or empty implants and were sacrificed on day 3,10 or 21. Cryostat kidney sections were histochemically stained for ecto-ADPase activity using enzyme-histochemistry and glomerular reaction product was quantitatively evaluated by computerized image analysis. Both the histological distribution of reaction product in each glomerulus, as reflectedby the relative glomerular area covered with reaction product, as well as enzyme activity, as reflected by staining intensity of thereaction product, were scored. The results show significantly decreased histological distribution after OVX; OVX, however, did not change enzyme activity. It further appeared that OE2 (partly) prevented the decrease of histological distribution in OVX rats, while the enzyme activity was significantly increased by exogenous OE2. In cyclic rats, OE2 did not change histological distribution, although OE2 significantly increased enzyme activity in these rats.It is concluded that glomerular ecto-ADPase expression in the rat kidney is influenced by one or more ovarian factor(s), a very likely candidate being oestradiol. These results may thus point to a dual action of OE2 upon haemostasis: In addition to the known enhancement of procoagulatory plasma factors by OE2, also anti-aggregatory effects may be stimulated by OE2 as reflected by upregulation of vessel wall associated ecto-ADPase activity.

scholarly journals Enzyme histochemistry on freeze-dried, resin-embedded tissue.

1989 ◽  
Vol 37 (5) ◽  
pp. 643-652 ◽  
Author(s):  
G I Murray ◽  
M D Burke ◽  
S W Ewen
Keyword(s):  
Enzyme Activity ◽  
Low Temperature ◽  
Enzyme Histochemistry ◽  
Aqueous Media ◽  
Histochemical Demonstration ◽  
Glycol Methacrylate ◽  
Tissue Sections ◽  
Freeze Dried ◽  
Wide Range ◽  
Tissue Specimens

We have developed a method for histochemical demonstration of a wide range of enzymes in freeze-dried, resin-embedded tissue. Freeze-dried tissue specimens were embedded without fixation at low temperature (4 degrees C or -20 degrees C) in glycol methacrylate resin or LR Gold resin. Enzyme activity was optimally preserved by embedding the freeze-dried tissue in glycol methacrylate resin. All enzymes studied (oxidoreductases, esterases, peptidases, and phosphatases), except for glucose-6-phosphatase, were readily demonstrated. The enzymes displayed high activity and were accurately localized without diffusion when tissue sections were incubated in aqueous media, addition of colloid stabilizers to the incubating media not being required. Freeze-drying combined with low-temperature resin embedding permits the demonstration of a wide range of enzymes with accurate enzyme localization, high enzyme activity, and excellent tissue morphology.

scholarly journals Enzyme Histochemistry of the Sheep Uterus During the Oestrous Cycle

1979 ◽  
Vol 32 (5) ◽  
pp. 409 ◽  
Author(s):  
M Javad Zamiri ◽  
Alan W Blackshaw
Keyword(s):  
Enzyme Activity ◽  
Enzyme Histochemistry ◽  
Oestrous Cycle ◽  
Alkaline Phosphatases ◽  
Acid And Alkaline Phosphatases

Enzyme histochemical techniques were applied to frozen sheep uteri from different stages of the oestrous cycle. The localization and activities of succinate, lactate, glucose-6-phosphate, and isocitrate (NADP+) dehyrogenases and acid and alkaline phosphatases were studied in the luminal and glandular epithelia, caruncle and myometrium. Enzyme activity in the sections was scored on a scale of 0-5.

Morphological and biochemical analyses of changes in rat hepatocytes related to wine and ethanol consumption

1984 ◽  
Vol 42 ◽  
pp. 232-233
Author(s):  
S.M. Geyer ◽  
C.L. Mendenhall ◽  
J.T. Hung ◽  
E.L. Cardell ◽  
R.L. Drake ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Enzyme Activity ◽  
Malic Enzyme ◽  
Smooth Endoplasmic Reticulum ◽  
Rat Hepatocytes ◽  
Mature Male ◽  
Treatment Groups ◽  
Malic Enzyme Activity ◽  
Biochemical Analyses

Thirty-three mature male Holtzman rats were randomly placed in 3 treatment groups: Controls (C); Ethanolics (E); and Wine drinkers (W). The animals were fed synthetic diets (Lieber type) with ethanol or wine substituted isocalorically for carbohydrates in the diet of E and W groups, respectively. W received a volume of wine which provided the same gram quantity of alcohol consumed by E. The animals were sacrificed by decapitation after 6 weeks and the livers processed for quantitative triglycerides (T3), proteins, malic enzyme activity (MEA), light microscopy (LM) and electron microscopy (EM). Morphometric analysis of randomly selected LM and EM micrographs was performed to determine organellar changes in centrilobular (CV) and periportal (PV) regions of the liver. This analysis (Table 1) showed that hepatocytes from E were larger than those in C and W groups. Smooth endoplasmic reticulum decreased in E and increased in W compared to C values.

Reductive light activation of enzyme activity

2000 ◽  
Vol 110 (3) ◽  
pp. 295-295
Author(s):  
Louise Anderson ◽  
Per Gardestrom
Keyword(s):  
Enzyme Activity ◽  
Light Activation
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