scholarly journals Enzyme histochemistry on freeze-dried, resin-embedded tissue.

1989 ◽  
Vol 37 (5) ◽  
pp. 643-652 ◽  
Author(s):  
G I Murray ◽  
M D Burke ◽  
S W Ewen
Keyword(s):  
Enzyme Activity ◽  
Low Temperature ◽  
Enzyme Histochemistry ◽  
Aqueous Media ◽  
Histochemical Demonstration ◽  
Glycol Methacrylate ◽  
Tissue Sections ◽  
Freeze Dried ◽  
Wide Range ◽  
Tissue Specimens

We have developed a method for histochemical demonstration of a wide range of enzymes in freeze-dried, resin-embedded tissue. Freeze-dried tissue specimens were embedded without fixation at low temperature (4 degrees C or -20 degrees C) in glycol methacrylate resin or LR Gold resin. Enzyme activity was optimally preserved by embedding the freeze-dried tissue in glycol methacrylate resin. All enzymes studied (oxidoreductases, esterases, peptidases, and phosphatases), except for glucose-6-phosphatase, were readily demonstrated. The enzymes displayed high activity and were accurately localized without diffusion when tissue sections were incubated in aqueous media, addition of colloid stabilizers to the incubating media not being required. Freeze-drying combined with low-temperature resin embedding permits the demonstration of a wide range of enzymes with accurate enzyme localization, high enzyme activity, and excellent tissue morphology.

scholarly journals Enzyme histochemistry on freeze-substituted glycol methacrylate-embedded tissue.

1990 ◽  
Vol 38 (1) ◽  
pp. 95-101 ◽  
Author(s):  
G I Murray ◽  
S W Ewen
Keyword(s):  
Low Temperature ◽  
Enzyme Histochemistry ◽  
Aqueous Media ◽  
Freeze Substitution ◽  
Histochemical Demonstration ◽  
Enzyme Localization ◽  
Glycol Methacrylate ◽  
Tissue Sections ◽  
Wide Range ◽  
Tissue Specimens

We developed a method for histochemical demonstration of a wide range of enzymes in freeze-substituted glycol methacrylate-embedded tissue. Tissue specimens were freeze-substituted in acetone and then embedded at low temperature in glycol methacrylate resin. All enzymes studied (oxidoreductases, hydrolases) were readily demonstrated. The enzymes displayed high activity and were accurately localized without diffusion when tissue sections were incubated in aqueous media, addition of colloid stabilizers to the incubating media not being required. Freeze-substitution combined with low-temperature glycol methacrylate embedding permits the demonstration of a wide range of enzymes with accurate enzyme localization, maintenance of enzyme activity, and excellent tissue morphology.

scholarly journals LOW TEMPERATURE EMBEDDING IN GLYCOL METHACRYLATE FOR ENZYME HISTOCHEMISTRY IN PLANT AND ANIMAL TISSUES

1972 ◽  
Vol 20 (12) ◽  
pp. 986-990 ◽  
Author(s):  
ANNE E. ASHFORD ◽  
WILLIAM G. ALLAWAY ◽  
MARGARET E. MCCULLY
Keyword(s):  
Enzyme Activity ◽  
Low Temperature ◽  
Enzyme Histochemistry ◽  
Animal Tissues ◽  
Glycol Methacrylate

A method is described by which enzyme activity can be retained in plant and animal tissues embedded in glycol methacrylate at low temperature.

scholarly journals Charge Compensation in Europium-Doped Hafnia Nanoparticles: Solvothermal Synthesis and Colloidal Dispersion

Crystals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1042
Author(s):  
Xavier H. Guichard ◽  
Francesco Bernasconi ◽  
Alessandro Lauria
Keyword(s):  
Low Temperature ◽  
Hafnium Oxide ◽  
Aqueous Media ◽  
Charge Compensation ◽  
Colloidal Dispersion ◽  
X Ray Diffraction ◽  
X Ray ◽  
Wide Range ◽  
Direct Formation ◽  
Enhanced Luminescence

Effective charge compensation of europium in hafnium oxide nanoparticles was achieved at low temperature, allowing high doping incorporation (up to 6 at.%) and enhanced luminescence. The efficiency of the incorporation and charge compensation was confirmed by scanning electron microscope energy dispersive X-ray spectroscopy and powder X-ray diffraction measurements. Despite the known polymorphism of hafnium oxide, when doped to a concentration above 3 at.%, only the pure monoclinic phase was observed up to 6 at.% of europium. Furthermore, the low-temperature solvothermal route allowed the direct formation of stable dispersions of the synthesized material over a wide range of concentrations in aqueous media. The dispersions were studied by diffuse light scattering (DLS) to evaluate their quality and by photoluminescence to investigate the incorporation of the dopants into the lattice.

scholarly journals Use of tissue-free glycol methacrylate sections as semi-permeable membranes: a simple way to shorten incubation times and to improve localization in enzyme histochemistry.

1989 ◽  
Vol 37 (2) ◽  
pp. 173-176 ◽  
Author(s):  
P O Gerrits ◽  
R W Horobin ◽  
M J Hardonk
Keyword(s):  
Alkaline Phosphatase ◽  
Azo Dye ◽  
Enzyme Histochemistry ◽  
Diazonium Salt ◽  
Histochemical Staining ◽  
Semipermeable Membranes ◽  
Large Molecules ◽  
Glycol Methacrylate ◽  
Tissue Sections ◽  
Antibody Staining

Placing 2-microns sections of tissue-free glycol methacrylate on top of tissue sections is a simple way of forming semipermeable membranes to enhance enzyme histochemical staining. For demonstrating alkaline phosphatase in glycol methacrylate-embedded kidney by a standard azo dye method, such membranes enabled incubation times to be reduced to 1-2 hr, with azo dye reaction product being more crisply localized as compared to sections stained without membranes. Such effects are possible because the membranes are highly permeable to small molecules (e.g., substrate and diazonium salt), slightly permeable to molecules of moderate size (e.g., the final reaction product), and impermeable to large molecules (e.g., alkaline phosphatase and other tissue biopolymers). The implications of these findings for enzyme histochemistry and for enzyme-labeled antibody staining are discussed.

scholarly journals VISUALIZATION IN FREEZE-DRIED TISSUE SECTIONS OF STRUCTURES TO BE STUDIED BY QUANTITATIVE HISTOCHEMISTRY

1971 ◽  
Vol 19 (3) ◽  
pp. 186-191
Author(s):  
BO HELLMAN ◽  
ÅKE LERNMARK
Keyword(s):  
Limiting Factor ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Quantitative Histochemistry ◽  
Tissue Sections ◽  
Freeze Dried ◽  
Tissue Specimens

The limiting factor for quantitative histochemistry employing freeze-dried tissue sections is the ability to identify morphologic structures. Satisfactory staining of freeze-dried tissue specimens intended for further microanalysis was achieved by using isopentane solutions of free dye bases. The practical details of this rapid histologic control are outlined and its usefulness is illustrated by measurements of fructose 1,6-diphosphate and insulin in microdissected specimens of pancreatic β cells.

scholarly journals RELaTED Project: New Developments on Ultra-Low Temperature District Heating Networks

Proceedings ◽  
2020 ◽  
Vol 65 (1) ◽  
pp. 25
Author(s):  
Antonio Garrido Marijuan ◽  
Roberto Garay ◽  
Mikel Lumbreras ◽  
Víctor Sánchez ◽  
Olga Macias ◽  
...  
Keyword(s):  
Low Temperature ◽  
High Performance ◽  
Waste Heat ◽  
District Heating ◽  
Hot Water ◽  
Thermal Systems ◽  
Heating Source ◽  
Wide Range ◽  
Thermal Sources ◽  
Heating Networks

District heating networks deliver around 13% of the heating energy in the EU, being considered as a key element of the progressive decarbonization of Europe. The H2020 REnewable Low TEmperature District project (RELaTED) seeks to contribute to the energy decarbonization of these infrastructures through the development and demonstration of the following concepts: reduction in network temperature down to 50 °C, integration of renewable energies and waste heat sources with a novel substation concept, and improvement on building-integrated solar thermal systems. The coupling of renewable thermal sources with ultra-low temperature district heating (DH) allows for a bidirectional energy flow, using the DH as both thermal storage in periods of production surplus and a back-up heating source during consumption peaks. The ultra-low temperature enables the integration of a wide range of energy sources such as waste heat from industry. Furthermore, RELaTED also develops concepts concerning district heating-connected reversible heat pump systems that allow to reach adequate thermal levels for domestic hot water as well as the use of the network for district cooling with high performance. These developments will be demonstrated in four locations: Estonia, Serbia, Denmark, and Spain.

scholarly journals Discrimination of Aspergillosis, Mucormycosis, Fusariosis, and Scedosporiosis in Formalin-Fixed Paraffin-Embedded Tissue Specimens by Use of Multiple Real-Time Quantitative PCR Assays

2016 ◽  
Vol 54 (11) ◽  
pp. 2798-2803 ◽  
Author(s):  
Elham Salehi ◽  
Mohammad T. Hedayati ◽  
Jan Zoll ◽  
Haleh Rafati ◽  
Maryam Ghasemi ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Aspergillus Flavus ◽  
Ffpe Tissue ◽  
Content Type ◽  
Real Time Quantitative Pcr ◽  
Tissue Sections ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Tissue Specimens ◽  
Formalin Fixed

In a retrospective multicenter study, 102 formalin-fixed paraffin-embedded (FFPE) tissue specimens with histopathology results were tested. Two 4- to 5-μm FFPE tissue sections from each specimen were digested with proteinase K, followed by automated nucleic acid extraction. Multiple real-time quantitative PCR (qPCR) assays targeting the internal transcribed spacer 2 (ITS2) region of ribosomal DNA, using fluorescently labeled primers, was performed to identify clinically important genera and species of Aspergillus , Fusarium , Scedosporium , and the Mucormycetes . The molecular identification was correlated with results from histological examination. One of the main findings of our study was the high sensitivity of the automated DNA extraction method, which was estimated to be 94%. The qPCR procedure that was evaluated identified a range of fungal genera/species, including Aspergillus fumigatus , Aspergillus flavus , Aspergillus terreus , Aspergillus niger , Fusarium oxysporum , Fusarium solani , Scedosporium apiospermum , Rhizopus oryzae , Rhizopus microsporus , Mucor spp., and Syncephalastrum . Fusarium oxysporum and F. solani DNA was amplified from five specimens from patients initially diagnosed by histopathology as having aspergillosis. Aspergillus flavus , S. apiospermum , and Syncephalastrum were detected from histopathological mucormycosis samples. In addition, examination of four samples from patients suspected of having concomitant aspergillosis and mucormycosis infections resulted in the identification of two A. flavus isolates, one Mucor isolate, and only one sample having both R. oryzae and A. flavus . Our results indicate that histopathological features of molds may be easily confused in tissue sections. The qPCR assay used in this study is a reliable tool for the rapid and accurate identification of fungal pathogens to the genus and species levels directly from FFPE tissues.

GLYCOL METHACRYLATE EMBEDDING AND STAIN MODIFICATIONS FOR BRAIN TISSUE SPECIMENS

1989 ◽  
Vol 48 (3) ◽  
pp. 312
Author(s):  
R. A. Brumback ◽  
D. L. Feeback ◽  
R. W. Leech ◽  
J. L. Ketring ◽  
J. J. Davis
Keyword(s):  
Brain Tissue ◽  
Glycol Methacrylate ◽  
Tissue Specimens
Sign in / Sign up

Export Citation Format

Share Document