scholarly journals Enzyme Histochemistry of the Sheep Uterus During the Oestrous Cycle

1979 ◽  
Vol 32 (5) ◽  
pp. 409 ◽  
Author(s):  
M Javad Zamiri ◽  
Alan W Blackshaw
Keyword(s):  
Enzyme Activity ◽  
Enzyme Histochemistry ◽  
Oestrous Cycle ◽  
Alkaline Phosphatases ◽  
Acid And Alkaline Phosphatases

Enzyme histochemical techniques were applied to frozen sheep uteri from different stages of the oestrous cycle. The localization and activities of succinate, lactate, glucose-6-phosphate, and isocitrate (NADP+) dehyrogenases and acid and alkaline phosphatases were studied in the luminal and glandular epithelia, caruncle and myometrium. Enzyme activity in the sections was scored on a scale of 0-5.

scholarly journals Uterine Endometrial Phosphomonoesterases in Relation to Implantation in the Ewe and Rabbit Doe

1970 ◽  
Vol 23 (4) ◽  
pp. 1089 ◽  
Author(s):  
SC Chambers
Keyword(s):  
Oestrous Cycle ◽  
Alkaline Phosphatases ◽  
Uterine Endometrium ◽  
Acid And Alkaline Phosphatases

The activities of acid and alkaline phosphatases in the uterine endometrium of the ewe and rabbit doe were studied during the oestrous cycle and pseudopregnancy and in relation to the implanting conceptus.

Progestation

1967 ◽  
Vol 56 (3_Suppla) ◽  
pp. S7-S45 ◽  
Keyword(s):  
Tritiated Thymidine ◽  
Succinic Dehydrogenase ◽  
Glandular Epithelium ◽  
Synthetic Activity ◽  
Cell Renewal ◽  
Pulse Labelling ◽  
Uterine Receptivity ◽  
Alkaline Phosphatases ◽  
Monocytic Cells ◽  
Acid And Alkaline Phosphatases

ABSTRACT Autoradiographic, enzymic and histologic studies on uteri of pregnant rats were carried out to follow the endometrial modifications which take place during progestation (days L0 – L4) and culminate in the state of uterine receptivity essential for ovum-implantation. Pulse labelling with tritiated thymidine (radioactive DNA precursor) on L0, L1 and L2 revealed a sequence of cell renewal in luminal and glandular epithelium and endometrial stroma. On L3 and L4 stromal cells showed extensive incorporation of tritiated thymidine. This synthetic activity was associated with endometrial preparation for decidualization and was evoked at least in part, by the surge of oestrogen on L3. All layers of the uterine wall were heavily infiltrated on L0 and resembled the site of an acute inflammatory reaction. Subsequently, polymorphonuclear infiltration diminished and monocytic cells predominated. On L3 a spatial arrangement was observed: eosinophiles were concentrated in the basal endometrium and monocytic cells in the subepithelial stroma. A comparison was made between such a shift in migratory cells in the uterus and similar phenomena which occur in inflammatory and immune reactions. Activities of acid and alkaline phosphatases, of ATP-ase and succinic dehydrogenase were low on L0 and L1 during the periods of infiltration, degeneration and regeneration of luminal and glandular epithelium. Enzymic activities increased on the following days, (L3 and L4). Vascular dilation and engorgement and endometrial oedema were observed near the blastocysts on L4. Most blastocysts incorporated tritiated thymidine after 14.00 h on L4, but some showed uptake before loss of the zona which occurs usually between 14.00 and 16.00 h; therefore, it was assumed that the permeability of the zona increases prior to being shed. Activities of succinic dehydrogenase and acid and alkaline phosphatases were demonstrable in blastocysts on L4 while they were still »free« in the uterine lumen.

Effects of Kanwa on Rat Gastrointestinal Phosphatases

2010 ◽  
Vol 3 (3) ◽  
pp. 1147-1152
Author(s):  
Jacob Bamaiyi ◽  
Omajali ◽  
Sanni Momoh
Keyword(s):  
Alkaline Phosphatase ◽  
Small Intestine ◽  
Acid Phosphatase ◽  
Sodium Carbonate ◽  
Elevated Level ◽  
Food Additive ◽  
Alkaline Phosphatases ◽  
Acid And Alkaline Phosphatases ◽  
Diagnostic Indices ◽  
High Level

This study investigates the effects of kanwa on rat gastrointestinal phosphatases. The rats were administered 7% w/v concentration of  trona (Kanwa) orally for a period of two weeks in order to investigate how this compound is being used as food additive in some homes in Nigeria. The Kanwa used in this study was the handpicked variety obtained from sellers from Anyigba market in eastern part of Kogi State, Nigeria. Kanwa, a hydrated sodium carbonate (Na2CO3NaHCO3.2H2O) was obtained as a dried lake salt. Acid phosphatase has the ability to dephosphorylate molecules containing phosphate group. The decreased and elevated level in serum or plasma acid and alkaline phosphatases serves as diagnostic indices for various diseases. Results showed that there was increase and decrease of acid phosphatase (ACP) activities in both the stomach and small intestine. The activities of alkaline phosphatase (ALP) fluctuated in the small intestine. However, in the stomach, an increase activity of ALP was noticed throughout the period of ‘Kanwa’ administration. We concluded that although the level of ‘Kanwa’ consumed in most homes may not be toxic if not taken continuously or repeatedly. Thus, continuous consumption should be discouraged as accumulation of high level of ‘Kanwa’ may cause damages or injuries to the various organs/tissues and may disrupt normal body function.

scholarly journals Detection of phosphatase activity in aquatic and terrestrial cyanobacterial strains

2013 ◽  
pp. 31-42
Author(s):  
Olivera Babic ◽  
Jelica Simeunovic ◽  
Natasa Skrbic ◽  
Dajana Kovac ◽  
Zorica Svircev
Keyword(s):  
Enzyme Activity ◽  
Specific Property ◽  
Enzyme Synthesis ◽  
Ecological Group ◽  
Extreme Conditions ◽  
Survival Strategy ◽  
Acid Phosphatases ◽  
Nitrogen Fixing ◽  
Cyanobacterial Strain ◽  
Alkaline Phosphatases

Cyanobacteria, as highly adaptable microorganisms, are characterized by an ability to survive in different environmental conditions, in which a significant role belongs to their enzymes. Phosphatases are enzymes produced by algae in relatively large quantities in response to a low orthophosphate concentration and their activity is significantly correlated with their primary production. The activity of these enzymes was investigated in 11 cyanobacterial strains in order to determine enzyme synthesis depending on taxonomic and ecological group of cyanobacteria. The study was conducted with 4 terrestrial cyanobacterial strains, which belong to Nostoc and Anabaena genera, and 7 filamentous water cyanobacteria of Nostoc, Oscillatoria, Phormidium and Microcystis genera. The obtained results showed that the activity of acid and alkaline phosphatases strongly depended on cyanobacterial strain and the environment from which the strain originated. Higher activity of alkaline phosphatases, ranging from 3.64 to 85.14 ?molpNP/s/dm3, was recorded in terrestrial strains compared to the studied water strains (1.11-5.96 ?molpNP/s/dm3). The activity of acid phosphatases was higher in most tested water strains (1.67-6.28 ?molpNP/s/dm3) compared to the activity of alkaline phosphatases (1.11-5.96 ?molpNP/s/dm3). Comparing enzyme activity of nitrogen fixing and non-nitrogen fixing cyanobacteria, it was found that most nitrogen fixing strains had a higher activity of alkaline phosphatases. The data obtained in this work indicate that activity of phosphatases is a strain specific property. The results further suggest that synthesis and activity of phosphatases depended on eco-physiological characteristics of the examined cyanobacterial strains. This can be of great importance for the further study of enzymes and mechanisms of their activity as a part of cyanobacterial survival strategy in environments with extreme conditions.

ALTERATION OF 3β-HYDROXYSTEROID DEHYDROGENASE ACTIVITY IN RAT OVARIES DURING THE OESTROUS CYCLE

1974 ◽  
Vol 60 (3) ◽  
pp. 441-443 ◽  
Author(s):  
A. M. GAWIENOWSKI ◽  
J. N. CLARK ◽  
C. N. SRINIVASAN
Keyword(s):  
Enzyme Activity ◽  
Dehydrogenase Activity ◽  
Hydroxysteroid Dehydrogenase ◽  
Oestrous Cycle

SUMMARY The activity of 3β-hydroxysteroid dehydrogenase has been determined during various stages of the oestrous cycle in rats. The enzyme activity was high during pro-oestrus and oestrus but low during dioestrus I and II.

scholarly journals Effect of heating oil on the activity of soil enzymes and the yield of yellow lupine

2011 ◽  
Vol 52 (No. 5) ◽  
pp. 220-226 ◽  
Author(s):  
J. Kucharski ◽  
E. Jastrzębska
Keyword(s):  
Alkaline Phosphatase ◽  
Soil Enzymes ◽  
Urease Activity ◽  
Alkaline Phosphatases ◽  
Acid And Alkaline Phosphatases ◽  
Heating Oil ◽  
Average Activity ◽  
Yellow Lupine ◽  
Experimental Soil ◽  
Limed Soil

The aim of the study was to determine the response of soil enzymes such as dehydrogenases, urease and acid and alkaline phosphatases to heating oil contaminating (0.0, 0.25, 0.5, 0.75, 1.0, 1.5% of soil) the experimental soil supplemented with lime and used for cultivation of yellow lupine of the Markiz variety. An increasing contamination of soil with heating oil stimulated the activity of dehydrogenases and acid and alkaline phosphatases but had a toxic effect on yellow lupine. Lime supplements did not have a significant effect on an average activity of soil dehydrogenases. However, such soil treatment had a significant effect on urease. Increasing heating oil doses in lime-supplemented soil stimulated urease activity, whereas in lime-free soil urease activity was inhibited. The activity of acid and alkaline phosphatase was lower in limed soil than in lime-free soil. The activity of dehydrogenases, urease and alkaline phosphatase in the soil with lupine cultivation was significantly higher than in the unsown soil.

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